Two Isophalerin Compounds from Ethyl Acetate of Leave and Fruit of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) and Its Antibacterial Activity

Susilawati Susilawati(1*), Sabirin Matsjeh(2), Harno Dwi Pranowo(3), Chairil Anwar(4)

(1) Department of Education of Mathematics and Natural Sciences, FKIP, Universitas Riau, Pekanbaru
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara PO BOX BLS 21 Yogyakarta 55281
(*) Corresponding Author


Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is belong to family of Thymelaeaceae is one of Indonesia's traditional medicines. The aim of this research is to isolate secondary metabolites from ethyl acetate extract of leave and fruit of mahkota dewa and to determine the molecular structure of isolated compounds using spectroscopic method and to know the antibacterial activity of the isolated compound. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The compounds were separated and purified with column chromatography. The compound 1 was isolated from ethyl acetate extract of leave as white needle amorphous solid as 45 mg. The compound was identified by spectroscopic as 4,6-dihydroxy-4’-methoxybenzophenon-2-O-β-D-glucopyranoside and named isophalerin B. From the test results of antibacterial activity showed that the compound 1 (10 mg/mL) in ethanol has a weak activity against the bacteria S. aureus and E. coli. The compound 2 was isolated from ethyl acetate extract of fruit as peach needle crystal as 10 mg. The compound was identified by spectroscopic as 4,6-dihydroxy-4’-methoxybenzophenon-2-O-α-D-glucopyranoside and named isophalerin A.


mahkota dewa; Phaleria macrocarpa; isophalerin; antibacterial; benzophenon

Full Text:

Full Text Pdf


[1] Harmanto, N., 2005, Mahkota dewa, Gods Heritage Drugs, 1st ed., Agro Media Pustaka, Jakarta.

[2] Sumastuti, R., and Sonlimar, M., 2006, Cytotoxic Effect of Fruit and Leaf Extracts Mahkota dewa [Phaleria macrocarpa (Scheff.) Boerl.] of Cells Hela, Pharmacology, Faculty of Medicine, Universitas Gadjah Mada, arsip/122002/art-3.htm, accessed on May 08th 2006.

[3] Lisdawati, V., 2007, Mahkota Dewa Fruit-toxicity, Antioxidant Effects, and Anticancer Effect by Pharmacological Screening Tests,, accessed on October 14th 2008.

[4] Suryani, L., and Stepriyani, S., 2007, Mutiara Medika, 7(1), 23–28.

[5] Oshimi, S., Zaima, K., Matsuno, Y., Hirasawa, Y., Iizuka, T., Studiawan, H., Indrayanto, G., Zaini, N.C., and Morita, H., 2008, J. Nat. Med., 62(2), 207–210.

[6] Hakim, R.W., Nawawi, A., Adnyana, I.K., Achmad, S.A., Makmur, L., Hakim, E.H., Sjah, Y.M., and Kitajima, M., 2004, Bull. Soc. Nat. Prod. Chem., 4, 67–70.

[7] Tambunan R.M., and Simanjuntak, P., 2006, Indones. J. Pharm., 17(4), 184–189.

[8] Winarno, H., and Katrin, W.E, 2009, Indones. J. Chem., 9(1), 142–145.

[9] Hartati, M.S., Mubarika, S., Gandjar, I.G., Hamann, M.T., Rao K.V., and Wahyuono, S., 2005, Indones. J. Pharm., 16(1), 51–57.

[10] Zhang, Y.B., Yu, X.J., and Liu H.M., 2006, J. Asian Nat. Prod. Res., 8(1-2), 119–123.

[11] Susilawati, Matsjeh, S., Pranowo, H. D., and Anwar, C., 2011, Indones. J. Chem., 11(2), 180–185.

[12] Ferrari, J., Terreaux, C., Sahpaz, S., Msonthi, J.D., Wolfender, L., and Hostettmann, K., 2000, Phytochemistry, 54(8), 883–889.

[13] Ramakrishnan, G., Kothai R., Jaykar B., and Rathnakumar, T.V., 2011, Int. J. PharmTech. Res., 3(2), 1000–1004.

[14] Dey, P.M., and Harborne, J.B., 1991, Methods in Plant Biochemistry: Assay for Bioactivity, Vol. 6, Academic Press Limited, London.

[15] Creswell, C.J., Runquist, O.A., and Campbelll, M.M., 2005, Spectral Analysis of Organic Compounds, 10th ed., ITB, Bandung.

[16] Silverstein, R.M., Bassler, G.C., and Morrill, T.C., 2005. Spectrometric Identification Organic Compounds, 7th ed., John Wiley and Sons Inc., New York.

[17] Ontengco, D.C., and Dayap, L.A., 1996, Invitro Antibacterial Activity of Rind Essential Oil of Citrus Grandis (L) Osbeck vs Common Clinical Pathogen, Proceeding ASOMPS VIII, 10-13 June 1996, Melaka, 231–235.

[18] Mattana, C.M., Satorres, S.E., Sosa, A., Fusco, M., and Alcará L.E., 2010, Braz. J. Microbiol., 41(3), 581–587.


Article Metrics

Abstract views : 718 | views : 1088

Copyright (c) 2015 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Indonesian Journal of Chemistry (ISSN 1411-9420 / 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Analytics View The Statistics of Indones. J. Chem.