Polyaniline-Invertase-Gold Nanoparticles Modified Gold Electrode for Sucrose Detection

https://doi.org/10.22146/ijc.21189

Fitriyana Fitriyana(1), Fredy Kurniawan(2*)

(1) Chemical Engineering Department, Politeknik Negeri Samarinda, Jl. Dr. Cipto Mangunkusumo, Samarinda 75131
(2) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(*) Corresponding Author

Abstract


Sucrose sensor has been made by deposited the active materials on the surface of gold electrode. The active materials, i.e. polyaniline (PANI), invertase and gold nanoparticles, were deposited step by step. Aniline polymerization were conducted electrochemically at potential -500 to 1000 mV using voltammetry method with sweep rate 50 mV/s for 20 cycles in HCl solution pH 1.5. The modified electrode obtained was immersed in invertase 1 M phosphate buffer solution pH 6. The invertase trapping in polyaniline was performed using the same condition as aniline polymerization. Then, gold nanoparticles were deposited on the polyaniline-invertase modified gold electrode using Layer by Layer (LbL) technique. The polyaniline-invertase-gold nanoparticles modified gold electrode obtained was used to measure sucrose solution. Electrochemical signal of polyaniline (PANI)-invertase-gold nanoparticles modified gold electrode is increase with sucrose concentration. The sensitivity and detection limit of the electrode are 0.4657 µA mm-2 mM-1 and 9 µM, respectively. No electrochemical interference signals from fructose and glucose have been observed in the sucrose measurement.

Keywords


sucrose; sensor; invertase; polyaniline; gold nanoparticles

Full Text:

Full Text Pdf


References

[1] Shekarchizadeh, H., Ensafi, A.A., and Kadivar, M., 2013, Mater. Sci. Eng., C, 33 (6), 3553–3561.

[2] Aytaç, S., Kuralay, F., Boyacı, İ.H., and Unaleroglu, C., 2011, Sens. Actuators, B, 160 (1), 405–411.

[3] Bisenberger, M., Bräuchle, C., and Hampp, N., 1995, Sens. Actuators, B, 28 (3), 181–189.

[4] Gülce, H., Çelebi, S.S., Özyörük, H., and Yildiz, A., 1995, J. Electroanal. Chem., 397 (1-2), 217–223.

[5] Mohammadi, H., Amine, A., Cosnier, S., and Mousty, C., 2005, Anal. Chim. Acta, 543 (1-2), 143–149.

[6] Soldatkin, O.O., Peshkova, V.M., Dzyadevych, S.V., Soldatkin, A.P., Jaffrezic-Renault, N., and El’skaya, A.V., 2008, Mater. Sci. Eng., C, 28 (5-6), 959–964.

[7] Kurniawan, F., Tsakova, V., and Mirsky, V.M., 2009, J. Nanosci. Nanotechnol., 9 (4), 2407–2412.

[8] Budipramana, Y., Suprapto, S., Ersam, T., and Kurniawan, F., 2014, ARPN J. Eng. Appl. Sci., 9 (11), 2074–2077.

[9] Kurniawan, F., 2008, New Analytical Applications of Gold Nanoparticles, Dissertation, University of Regensburg, Germany.

[10] Krishnamoorthy, S., 2015, Curr. Opin. Biotechnol., 34, 118–124.

[11] Bu, L., Shen, B., and Cheng, Z., 2014, Adv. Drug Delivery Rev., 76, 21–38.

[12] Kurniawan, F., Tsakova, V., and Mirsky, V.M., 2006, Electroanalysis, 18 (19-20), 1937–1942.

[13] Dhand, C., Das, M., Datta, M., and Malhotra, B.D., 2011, Biosens. Bioelectron., 26 (6), 2811–2821.

[14] Modarresi-Alam, A.R., Amirazizi, H.A., Movahedifar, F., Farrokhzadeh, A., Asli, G.R., and Nahavandi, H., 2015, J. Mol. Struct., 1083, 17–26.

[15] Yu, E.H., and Sundmacher, K., 2007, Proc. Saf. Environ. Prot., 85, 489–493.

[16] Ivanov, S., Kurniawan, F., Tsakova, V., and Mirsky, V.M., 2009, Macromol. Mater. Eng., 294 (6-7), 441–444.



DOI: https://doi.org/10.22146/ijc.21189

Article Metrics

Abstract views : 2150 | views : 2469


Copyright (c) 2015 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.