Antioxidant Activity of Moringa oleifera Extracts

Wiwit Denny Fitriana(1), Taslim Ersam(2), Kuniyoshi Shimizu(3), Sri Fatmawati(4*)

(1) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111 Faculty of Mathematics and Natural Sciences, Universitas Pesantren Tinggi Darul Ulum, Jombang 61481
(2) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(3) Department of Agro-environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581
(4) Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111
(*) Corresponding Author


Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.


Moringa oleifera leaves; antioxidant; DPPH; ABTS

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