Biodecolorization and Biodegradation of Methylene Blue Dye by  Gloeophyllum trabeum  and Fenton-Like Reaction

Adi Setyo Purnomo; Hamdan Dwi Rizqi; Wahyu Adhy Kuncoro; Alya Awinatul Rohmah; Yuji Tsutsumi

doi:10.22146/ijc.109527

Biodecolorization and Biodegradation of Methylene Blue Dye by Gloeophyllum trabeum and Fenton-Like Reaction

https://doi.org/10.22146/ijc.109527

Adi Setyo Purnomo^(1*), Hamdan Dwi Rizqi⁽²⁾, Wahyu Adhy Kuncoro⁽³⁾, Alya Awinatul Rohmah⁽⁴⁾, Yuji Tsutsumi⁽⁵⁾

(1) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(2) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(3) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(4) Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember (ITS), Kampus ITS Sukolilo, Surabaya 60111, Indonesia
(5) Department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Motooka 744, Nishi-ku, Fukuoka 819-0395, Japan
(*) Corresponding Author

Abstract

This study investigated the potential of the brown-rot fungus Gloeophyllum trabeum to decolorize and degrade methylene blue (MB) through a synergistic Fenton-like mechanism. The main objective was to elucidate the contribution of the Fe²⁺-dependent Fenton-like reaction of G. trabeum in enhancing the efficiency of MB biodegradation. Decolorization experiments were carried out in mineral salt medium (MSM) with and without FeSO₄ supplementation for 0, 7, 14, 21, and 28 d of incubation. The results showed that the addition of Fe²⁺ significantly accelerated MB decolorization, achieving 91.9% efficiency compared to 88.3% without Fe²⁺. LC–MS analysis identified two major degradation metabolites, C₁₄H₁₆N₂O₃S and C₁₂H₁₁N₃O₇S, indicating oxidative demethylation and thiazine-ring cleavage as key degradation pathways. The findings suggest that G. trabeum employs a dual mechanism involving both Fenton-like radical generation and enzymatic oxidation, where Fe²⁺ addition further enhances its decolorization and degradation capability. Hence, this study demonstrates that the Fenton-like reaction of G. trabeum represents an effective and environmentally sustainable approach for the remediation of synthetic dye pollutants.

Keywords

biodecolorization; Fenton reaction; methylene blue; Gloeophyllum trabeum; pollutants

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DOI: https://doi.org/10.22146/ijc.109527