A Synergistic Effect of Free Radical Scavenging Activity of the Combination of  Mangifera indica  and  Euphorbia hirta  Extracts

I Made Wisnu Adhi Putra; Nyoman Suarjana; Mahfur Mahfur; Hasnawati Hasnawati

doi:10.22146/ijc.104706

A Synergistic Effect of Free Radical Scavenging Activity of the Combination of Mangifera indica and Euphorbia hirta Extracts

https://doi.org/10.22146/ijc.104706

I Made Wisnu Adhi Putra^(1*), Nyoman Suarjana⁽²⁾, Mahfur Mahfur⁽³⁾, Hasnawati Hasnawati⁽⁴⁾

(1) Department of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Jl. Raya Padang Luwih, Badung 80361, Bali, Indonesia
(2) Department of Public Health, Faculty of Health and Science, Universitas Dhyana Pura, Jl. Raya Padang Luwih, Badung 80361, Bali, Indonesia
(3) Department of Pharmacy, Faculty of Pharmacy, Universitas Pekalongan, Jl. Sriwijaya No. 3, Bendan, Pekalongan 51119, Indonesia
(4) Department of Pharmacy, Faculty of Pharmacy, Universitas Halu Oleo, Jl. H.E.A. Mokodompit, Kendari 93232, Indonesia
(*) Corresponding Author

Abstract

Due to its synergistic effect, the extract-extract combination is essential in traditional medicine formulation. The combination of Mangifera indica and Euphorbia hirta extracts can produce a synergistic effect if combined in a proper ratio. This investigation reported the synergistic antioxidant effect of M. indica and E. hirta. The ethanolic extracts of M. indica leaf (MILE) and E. hirta aerial part (EHAE) were acquired by the maceration method, followed by evaporation to remove the solvent. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) tests were used to assess the extracts' capacity to scavenge radicals. MILE and EHAE were combined with a ratio of 1:3 (comb. 1), 1:1 (comb. 2), and 3:1 (comb. 3). This work disclosed that the extract combinations showed all types of interactions. However, of all combinations, only comb. 3 depicted the synergistic interaction caused by the regeneration effect of EHAE on MILE. This finding has important implications for health, particularly disease prevention. M. indica and E. hirta extracts with high phenolic and flavonoid contents can work together to reduce oxidative stress and protect the body from a variety of diseases.

Keywords

extract combination; Euphorbia hirta; Mangifera indica; regeneration; synergistic effect

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DOI: https://doi.org/10.22146/ijc.104706