Identification of Anti-Inflammatory Components from  Launaea sarmentosa  Using  In Vitro  Cell Model

Thanh Quoc Chau Nguyen; Khang Thanh Vo; Cua Dinh Duong; Toan Phu Huynh; Lieu Thi Thuy Ca; Giao Huynh Dang

doi:10.22146/ijc.103167

Identification of Anti-Inflammatory Components from Launaea sarmentosa Using In Vitro Cell Model

https://doi.org/10.22146/ijc.103167

Thanh Quoc Chau Nguyen^(1*), Khang Thanh Vo⁽²⁾, Cua Dinh Duong⁽³⁾, Toan Phu Huynh⁽⁴⁾, Lieu Thi Thuy Ca⁽⁵⁾, Giao Huynh Dang⁽⁶⁾

(1) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(2) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(3) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(4) Department of Chemistry, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam; Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(5) Medicinal Chemistry Laboratory, CTU Hi-tech Building, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(6) College of Engineering, Can Tho University, Campus II, 3/2 Street, Can Tho 94000, Vietnam
(*) Corresponding Author

Abstract

Launaea sarmentosa (Willd.) Kuntze, a medicinal herb known for treating inflammatory diseases, was examined for its anti-inflammatory compounds to identify novel therapies. This study indicated that hexane and ethyl acetate fractional extracts significantly reduced NO secretion in LPS-stimulated RAW264.7 macrophages, indicating the presence of potential anti-inflammatory compounds. Additionally, four anti-inflammatory compounds, including taraxasteryl acetate (1), esculetin (2), pyrimidine-2,4-dione (3), and 5-hydroxypryrolidin-2-one (4), were isolated, and their structures were characterized using 1D and 2D-NMR. This study marked the first report of taraxasteryl acetate, pyrimidine-2,4-dione, and 5-hydroxypryrolidin-2-one being isolated from this species. Furthermore, these compounds exerted their anti-inflammatory role by inhibiting NO production and TNF-α expression. Thus, this study contributes to identifying anti-inflammatory constituents from L. sarmentosa and highlights a potential approach for developing phytotherapeutic agents.

Keywords

anti-inflammatory; Asteraceae; Launaea sarmentosa; isolation; RAW264.7 macrophages

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DOI: https://doi.org/10.22146/ijc.103167