Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Quantitative Analysis of L-Arginine in Freeze-Dried Red Dragon Fruits ( Hylocereus polyhizus )

Wan Mazlina Md Saad; Mohd Qamarul Aizat Isha; Muhamad Faizzudin Mohamad Zan; Siti Mastura Hanim Sallehoddin

doi:10.22146/ijc.100908

Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) Quantitative Analysis of L-Arginine in Freeze-Dried Red Dragon Fruits (Hylocereus polyhizus)

https://doi.org/10.22146/ijc.100908

Wan Mazlina Md Saad^(1*), Mohd Qamarul Aizat Isha⁽²⁾, Muhamad Faizzudin Mohamad Zan⁽³⁾, Siti Mastura Hanim Sallehoddin⁽⁴⁾

(1) Center for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia
(2) Center for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia
(3) Center for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia
(4) Center for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Puncak Alam, Selangor, Malaysia
(*) Corresponding Author

Abstract

Hylocereus polyrhizus is an exotic fruit with distinct bright colors and tastes that make it popular among many regions of the world. The fruit is cultivated worldwide and firmly established as a source of nutrition, thus, making good value in terms of its potential harvesting by various methods. However, prior reviews have documented weak scientific backing sparsely around extraction efficiency and favorable conditions for optimal yield. This study aimed to quantify the crude L-arginine content for both kinds of dragon fruiting by an RP-HPLC isocratic method, thus establishing a reliable and efficient approach for their detection. This study has revealed that short extraction times are beneficial in retaining L-arginine content since more extended extractions can result in the degradation of other components. Thus, this study opens further avenues toward exploring red dragon fruit as a natural source of L-arginine and advancing functional and nutraceutical products.

Keywords

L-arginine; red dragon fruit; RP-HPLC

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DOI: https://doi.org/10.22146/ijc.100908