The Efficacy of Mycosorb in Broiler Diets Contaminated with Low Doses of Aflatoxin B1

https://doi.org/10.21059/buletinpeternak.v45i3.64417

Catootjie Lusje Nalle(1*), Max Arthur Julian Supit(2), Ignasius Juncen Riu Bere(3), Roni Hawu(4)

(1) Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture, Kupang, 85228, Indonesia
(2) Animal Production Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture, Kupang, 85228, Indonesia
(3) Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture, Kupang, 85228, Indonesia
(4) Feed Technology Study Program, Department of Animal Husbandry, State Polytechnic of Agriculture, Kupang, 85228, Indonesia
(*) Corresponding Author

Abstract


The objective of the present study was to evaluate the efficacy of Mycosorb in broiler diets containing a low level of aflatoxin B1 (AFB1). A total of 200 male broiler chicks (Lohmann) were randomly distributed into 20 pens (10 birds/pen). The experimental design used was a 2 x 2 factorial completely randomized design with two main factors which were the AFB1 levels (non-detectable level; 2.58 ppb) and mycotoxin binder (MB) (0 and 0.15% Mycosorb), respectively. The treatments were control diet (P1), control diet + MB (P2), 2.58 ppb AFB1 diet (P3), and 2.58 ppb AFB1 diet + MB (P3). The AFB1 diets were formulated by replacing the whole proportion of fresh corn with moldy corn containing 4.22 ppb AFB1. The results showed that except for the digestibility coefficient of crude fat (DCCF), AL x MB interaction was not significant (P>0.05) for the growth performance and DCCP. The AFB1 levels (AL) improved (P<0.001) feed intake (FI), feed conversion ratio (FCR), and reduced the DCCF of broilers. The AFB1 levels enhanced the body weight gain (BWG) of growing broilers, but it did not augmented (P>0.05) the BWG of starter broilers. The digestibility coefficient of crude protein was not influenced (P>0.05) by the AFB1 levels. DCCF of broilers who received AFB1 diets were lower (P<0.05) than that of the control diet. Mycosorb did not affect (P>0.05) all variables measured. In conclusion, 1) except for DCCF, AFB1 levels x MB interaction did not improve growth performance and DCCP;  2) the AFB1 level of 2.58 ppb in the diets increased FI and BWG of broilers, but reduced the feed efficiency and DCCF; and 3) Mycosorb did not improve all variables measured.


Keywords


Aflatoxin; Broilers; Growth performance; Mycotoxin binder; Nutrient digestibility

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References

Aly, S. A. and W. Anwer. 2009. Effect of naturally contaminated feed with aflatoxins on performance of laying hens and the carryover of aflatoxin B residues in table eggs. Pakistan J. Nut. 8: 181-186. http://dx.doi.org/10.3923/pjn.2009.181.186

AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. Off. Anal. Chem., Arlington.

Indonesian National Standardization Board. 2015a. Part 1 : pre starter. SNI 8173.1:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.1-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-sebelum-masa-awal-pre-starter.pdf. Accessed February 2021.

Indonesian National Standardization Board. 2015b. Part 2 : starter. SNI 8172.2:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.2-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-masa-awal-starter.pdf. Accessed February 2021.

Indonesian National Standardization Board. 2015c. Part 3 : finisher. SNI 8173.3:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.3-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-masa-akhir-finisher.pdf. Accessed February 2021.

Benkerroum, N. 2020.Chronic and acute toxicities of aflatoxins: mechanisms of action. Int. J.Env. Res Pub. Health, 17: 423. http://doi.org/10.3390/ijerph17020423

Bronner, F. and D. Pansu. 1999. Nutritional aspects of calcium absorption. J. Nutr. 129: 9-12.

Bryden, W. L., R.B. Cumming and A.B. Lloyd. 2007. Sex and strain responses to aflatoxin B1 in the chicken. Avian Path. 9(4): 539-550.https://doi.org/10.1080/03079458008418441

Campos-M, M. and R. Campos-C. 2017. Application of quartering method in soils and foods. Int. J. Eng. Res. App. 7: 35-39.http://doi.org/10.9790/9622-0701023539

Carvajal-moreno, M. 2015. Metabolic Changes of Aflatoxin B1 to become an active carcinogen and the control of this toxin. Immunome Res. 11: 1–14.http:///doi.org/10.4172/1745-7580. 10000104.

Coppock, R. W., R. G. Christian, and B. J. Jacobsen. 2018. Aflatoxins. In: Veterinary Toxicology. Chapter 69. https://doi.org/10.1016/B978-0-12-811410-0.00069-6.

Denli, M. and F. Okan. 2006. Efficacy of different adsorbent in reducing the toxic effects of aflatoxin B1 in broiler diets. South African J. Anim. Sci. 36: 222-228.

Dersjant-Li, Y., M. W. A. Verstegen, and W. J. J. Gerrits. 2003. The impact of low concentrations of aflatoxin, deoxynivalenol or fumonisin in diets on growing pigs and poultry. Nutr. Res. Rev. 16: 223–239.

Diaz, G. J., H. W. Murcia, S. M. Cepeda, and H. J. Boermans. 2010. The role of selected cytochrome P450 enzymes on the bioactivation of aflatoxin B1 by duck liver microsomes. Avian Path. 39: 279-285, http://doi.org/10.1080/03079457.2010.495109.

Diaz, G. J., E. Calabrese, and R. Blain. 2008. Aflatoxicosis in chickens (Gallus gallus): An example of hormesis?. Poult. Sci. 87: 727–732. doi:10.3382/ps.2007-00403.

Ferket, P. R., and A. G. Gernat. 2006. Factors that affect feed intake of meat birds: a review. Int. J. Poult. Sci. 5: 905–911. https://doi.org/10.3923/ijps.2006.905.911

Fouad, A. M., D. Ruan, H. K. El-Senonseuj, W. Chen, S. Jiang, and C. Zheng. 2019. Harmful effects and control strategies of aflatoxin B1 produced by Aspergillus flavus and Aspergillus parasiticus strains on poultry: review. Toxins. 11: 176. http://doi.org/10.3390/toxins11030176

Gacem, M. A. and A. O. E. l. Hadj-Khelil. 2016. Toxicology, biosynthesis, bio-control of aflatoxin and new methods of detection. Asian Pac. J. Trop. Biomed. 6: 808-814. http://dx.doi.org/10.1016/j.apjtb.2016.07.012

Galarza-Seeber, R., J. D. Latorre, L. R. Bielke, V. A. Kuttappan, A. D. Wolfenden, X. Hernandez-Velasco, R. Merino-Guzman, J. L. Vicente, A. Donoghue, D. Cross, B. M. Hargis, and G. Tellez. 2016. Leaky gut and mycotoxins: Aflatoxin B1 does not increase gut permeability in broiler chickens. Front. Vet. Sci. 3: 10. http://doi.org/10.3389/fvets.2016.00010.

Girish, C. K. and G. Devegowda. 2006. Efficacy of glucomannan-containing yeast product (Mycosorb®) and hydrated sodium calcium aluminosilicate in preventing the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers. Asian-Australasian J. Anim. Sci. 19: 877-883.https://www.animbiosci.org/upload/pdf/143.pdf

Kan, C. A., R. Rump, and J. Kosutzky. 1989. Low level exposure of broilers and laying hens to aflatoxin B1, from naturally contaminated corn. Arch. Geflügelk. 53: 204-206.

Khan, B. A., S. Shahid, H. Mansoor, and A. Ahmed. 1990. Response of three commercial broiler chicken strains to aflatoxin. J. Islamic Acad. Sci. 3: 27−29

Kumar, S., P. K. Singh, A. Prasad, and Chadramoni. 2009. Effect of graded level of dietary energy and protein on growth performance of cockerels. Indian J. Anim. Nutr. 26: 86-89.

Kumar, V. V. 2018. Aflatoxins: properties, toxicity and detoxification. Nutri. Food. Sci. Int. J. 6: 555-696. DOI: 10.19080/NFSIJ.2018.06.555696.

Kurniasih and Y. A. Prakoso. 2019. Recent update: effects of aflatoxin in broiler chickens. J. World’s Poult. Res. 9: 68-77. http://doi.org/10.36380/jwpr.2019.8.

Latimer, G. W. 2012. AOAC International. Official methods of analysis of AOAC International. 19th edn. AOAC International. Gaithersburg, MD.

Marchioro, A. A., A. O. Mallmann, A. Diel, P. Dilkin, R. H. Rauber, F. J. H. Blazquez, C. A. Mallmann, A. Marchioro, A. E. A. O. Mallmann, A. A. Diel, P. Dilkin, R. H. Rauber, F. J. H. Blasquez, and M. G. A. Mallmann. 2013. Effects of Aflatoxins on Performance and Exocrine Pancreas of Broiler Chickens. Avian Dis. 57: 280–284.http://dx.doi.org/10.1637/10426-101712-Reg.1

McDonald, P., R. A. Edwards. J. F. D. Greenhalgh, and C. A. Morgan. 2002. Animal Nutrition. 6th edn. Prentice Hall, United Kingdom.

Massomo, S. M. S. 2020. Aspergillus flavus and aflatoxin contamination in the maize value chain and what needs to be done in Tanzania. Sci. African. 10e00606: 1-17. https://doi.org/10.1016/j.sciaf.2020.e00606

Mogadam, N. and A. Azizpour. 2011. Ameliorative effect of glucomannan-containing yeast products (Mycosorb) and sodium bentonite on performance and antibody titers against Newcastle disease in broilers during chronic aflatoxicosis. African J. Biotech. 10: 17372-17378. http://doi.org/10.5897/AJB11.238.

Monson, M., R. Coulombe, and K. Reed. 2015. Aflatoxicosis: Lessons from Toxicity and Responses to Aflatoxin B1. Poult. Agr. 5: 742–777. http://doi.org/10.3390/agriculture5030742

Moran, C. A., J. Apalajahti, A. Yiannikouris, S. Ojanpera, and H. Kettunen. 2013. Effects of low dietary aflatoxin B1 on broiler liver concentration without and with Mycosorb® toxin binder. J. Appl. Anim. Nut. 2: 1-3.

Nalle, C. L., A. H. Angi, M. A. J. Supit, S. Ambarwati, and N. S. Yuliani. 2019. Response of broiler chickens fed on diets containing low level of aflatoxin (B1 and B2) and supplemented with mycotoxin binder. J. Kajian Vet. 98-102. http://doi.org/10.35508/jkv.v0i0.1592.

Nalle, C. L., A. H. Angi, M. A. J. Supit, S. Ambarwati, and N. S. Yuliani. 2021. The performance, nutrient digestibility, aflatoxin B1 residue, and histopathological changes of broilers exposed to dietary mycosorb. Trop. Anim. Sci. J. 44: 160-172. http://doi.org/10.5398/tasj.2021.44.2.160.

Nalle, C. L., Helda, D. Kusumaningrum, J. Pobas, A. A. Lay Riwu and M. A. J. Supit. 2020. The Supplementation effects of multienzymes and synbiotics on production performance, nutrient utilization, economic value and salmonella spp. content of broilers. Pakistan J. Nut. 19: 51-60. https://dx.doi.org/10.3923/pjn.2020.51.60

Nalle, C. L., V. Ravindran, and G. Ravindran. 2012. Nutritional value of White Lupin (Lupinus albus) for Broilers: apparent metabolisable energy, apparent ileal amino acid digestibility and production performance. Anim. 6: 579-585. https://doi.org/10.1017/S1751731111001686.

Nazarizadeh, H. and J. Pourreza. 2019. Evaluation of three mycotoxin binders to prevent the adverse effects of aflatoxin B 1 in growing broilers. J. Appl. Anim. Res. 47: 135–139.http://doi.org/10.1080/09712119.2019.1584106

Neves, D. P., T. M. Banhazi, and I. A. Nääs. 2014. Feeding behaviour of broiler chickens: A review on the biomechanical characteristics. Barzillian J. Poult. Sci. 16: 1–16. https://doi.org/10.1590/1516-635x16021-16

Njoki, T. S. 2019. The effects of aflatoxin contaminated feed with or without a commercial binder on broiler performance. Egerton University, Kenya. http://ir-library.egerton.ac.ke/jspui/bitstream/123456789/1963/1/The%20effects%20of%20aflatoxin%20contaminated%20feed%20with%20or%20without%20a%20commercial%20binder%20on%20broiler%20performance.PDF

Osborne, D. J. and P. B. Hamilton. 1981. Decreased pancreatic digestive enzymes during aflatoxicosis. Poult. Sci. 60: 1818-1824.

Peng, X., S. Bai, X. Ding, Q. Zeng, K. Zhang, and J. Fang. 2015. Pathological changes in the immune organs of broiler chickens fed on corn naturally contaminated with aflatoxins B1 and B2. Avian Path. 44: 192-199. https://doi.org/10.1080/03079457.2015.1023179

Proszkowiec-Weglarz, M. and R. Angel. 2013. Calcium and phosphorus metabolism in broilers: Effect of homeostatic mechanism on calcium and phosphorus digestibility. J. Appl. Poult. Res. 22: 609–627 http://dx.doi.org/ 10.3382/japr.2012-00743.

Saki, A., A. Rahmani, H. Mahmoudi, M. M. Tabatabaei, P. Zamani, and A. R. Khosravi. 2018. The ameliorative effect of mycosorb in aflatoxin contaminated diet of broiler chickens. J. Livest. Sci. Technol. 6: 39-47. http://doi.org/0.22103/jlst.2018.8827.1154

Shuaib, F. M. B., J. Ehiri, A. Abdullahi, J. H. Williams, and P. E. Jolly. 2010. Reproductive health effects of aflatoxins : A review of the literature. Reprod. Toxicol. 29: 262–270. http://doi.org/10.1016/j.reprotox.2009.12.005.

Sineque, A. R., C. L. Macuamule, and F. R. Dos Anjos. 2017. Aflatoxin B1 Contamination in Chicken Livers and Gizzards from Industrial and Small Abattoirs, Measured by ELISA Technique in Maputo, Mozambique. Int. J. Env. Res. Pub. Health. 14: 951

Suganthi, R. U., K. P. Suresh, and R. Parvatham. 2011. Effect of Aflatoxin on Feed Conversion Ratio in Broilers: A Meta-analysis. Asian-Aust. J. Anim. Sci. 24: 1757 – 1762. http://dx.doi.org/10.5713/ajas.2011.11124

Yang, J., F. Bai, K. Zhang, X. Lv, S. Bai, L. Zhao, X. Peng, X. Ding, Y. Li, and J. Zhang. 2012. Effects of feeding corn naturally contaminated with AFB1 and AFB2 on performance and aflatoxin residue in broilers. Czech J. Anim. Sci. 57: 506-515.

Yunus, A.W., E. Razzazi_Fazeli, and J. Bohm. 2011. Aflatoxin B1 affecting broiler’s performance, immunity and gastrointestinal tracts: a review of history and contemporary issues. Toxins. 3: 566-590. http://doi.org/10.3390/toxins3060566

Aly, S. A. and W. Anwer. 2009. Effect of naturally contaminated feed with aflatoxins on performance of laying hens and the carryover of aflatoxin B residues in table eggs. Pakistan J. Nut. 8: 181-186. http://dx.doi.org/10.3923/pjn.2009.181.186

AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. Off. Anal. Chem., Arlington.

Indonesian National Standardization Board. 2015a. Part 1 : pre starter. SNI 8173.1:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.1-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-sebelum-masa-awal-pre-starter.pdf. Accessed February 2021.

Indonesian National Standardization Board. 2015b. Part 2 : starter. SNI 8172.2:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.2-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-masa-awal-starter.pdf. Accessed February 2021.

Indonesian National Standardization Board. 2015c. Part 3 : finisher. SNI 8173.3:2015. http://pakan.ditjenpkh.pertanian.go.id/wp-content/uploads/2019/08/SNI-8173.3-2015-Pakan-ayam-ras-pedaging-broiler-Bagian-2-masa-akhir-finisher.pdf. Accessed February 2021.

Benkerroum, N. 2020.Chronic and acute toxicities of aflatoxins: mechanisms of action. Int. J.Env. Res Pub. Health, 17: 423. http://doi.org/10.3390/ijerph17020423

Bronner, F. and D. Pansu. 1999. Nutritional aspects of calcium absorption. J. Nutr. 129: 9-12.

Bryden, W. L., R.B. Cumming and A.B. Lloyd. 2007. Sex and strain responses to aflatoxin B1 in the chicken. Avian Path. 9(4): 539-550.https://doi.org/10.1080/03079458008418441

Campos-M, M. and R. Campos-C. 2017. Application of quartering method in soils and foods. Int. J. Eng. Res. App. 7: 35-39.http://doi.org/10.9790/9622-0701023539

Carvajal-moreno, M. 2015. Metabolic Changes of Aflatoxin B1 to become an active carcinogen and the control of this toxin. Immunome Res. 11: 1–14.http:///doi.org/10.4172/1745-7580. 10000104.

Coppock, R. W., R. G. Christian, and B. J. Jacobsen. 2018. Aflatoxins. In: Veterinary Toxicology. Chapter 69. https://doi.org/10.1016/B978-0-12-811410-0.00069-6.

Denli, M. and F. Okan. 2006. Efficacy of different adsorbent in reducing the toxic effects of aflatoxin B1 in broiler diets. South African J. Anim. Sci. 36: 222-228.

Dersjant-Li, Y., M. W. A. Verstegen, and W. J. J. Gerrits. 2003. The impact of low concentrations of aflatoxin, deoxynivalenol or fumonisin in diets on growing pigs and poultry. Nutr. Res. Rev. 16: 223–239.

Diaz, G. J., H. W. Murcia, S. M. Cepeda, and H. J. Boermans. 2010. The role of selected cytochrome P450 enzymes on the bioactivation of aflatoxin B1 by duck liver microsomes. Avian Path. 39: 279-285, http://doi.org/10.1080/03079457.2010.495109.

Diaz, G. J., E. Calabrese, and R. Blain. 2008. Aflatoxicosis in chickens (Gallus gallus): An example of hormesis?. Poult. Sci. 87: 727–732. doi:10.3382/ps.2007-00403.

Ferket, P. R., and A. G. Gernat. 2006. Factors that affect feed intake of meat birds: a review. Int. J. Poult. Sci. 5: 905–911. https://doi.org/10.3923/ijps.2006.905.911

Fouad, A. M., D. Ruan, H. K. El-Senonseuj, W. Chen, S. Jiang, and C. Zheng. 2019. Harmful effects and control strategies of aflatoxin B1 produced by Aspergillus flavus and Aspergillus parasiticus strains on poultry: review. Toxins. 11: 176. http://doi.org/10.3390/toxins11030176

Gacem, M. A. and A. O. E. l. Hadj-Khelil. 2016. Toxicology, biosynthesis, bio-control of aflatoxin and new methods of detection. Asian Pac. J. Trop. Biomed. 6: 808-814. http://dx.doi.org/10.1016/j.apjtb.2016.07.012

Galarza-Seeber, R., J. D. Latorre, L. R. Bielke, V. A. Kuttappan, A. D. Wolfenden, X. Hernandez-Velasco, R. Merino-Guzman, J. L. Vicente, A. Donoghue, D. Cross, B. M. Hargis, and G. Tellez. 2016. Leaky gut and mycotoxins: Aflatoxin B1 does not increase gut permeability in broiler chickens. Front. Vet. Sci. 3: 10. http://doi.org/10.3389/fvets.2016.00010.

Girish, C. K. and G. Devegowda. 2006. Efficacy of glucomannan-containing yeast product (Mycosorb®) and hydrated sodium calcium aluminosilicate in preventing the individual and combined toxicity of aflatoxin and T-2 toxin in commercial broilers. Asian-Australasian J. Anim. Sci. 19: 877-883.https://www.animbiosci.org/upload/pdf/143.pdf

Kan, C. A., R. Rump, and J. Kosutzky. 1989. Low level exposure of broilers and laying hens to aflatoxin B1, from naturally contaminated corn. Arch. Geflügelk. 53: 204-206.

Khan, B. A., S. Shahid, H. Mansoor, and A. Ahmed. 1990. Response of three commercial broiler chicken strains to aflatoxin. J. Islamic Acad. Sci. 3: 27−29

Kumar, S., P. K. Singh, A. Prasad, and Chadramoni. 2009. Effect of graded level of dietary energy and protein on growth performance of cockerels. Indian J. Anim. Nutr. 26: 86-89.

Kumar, V. V. 2018. Aflatoxins: properties, toxicity and detoxification. Nutri. Food. Sci. Int. J. 6: 555-696. DOI: 10.19080/NFSIJ.2018.06.555696.

Kurniasih and Y. A. Prakoso. 2019. Recent update: effects of aflatoxin in broiler chickens. J. World’s Poult. Res. 9: 68-77. http://doi.org/10.36380/jwpr.2019.8.

Latimer, G. W. 2012. AOAC International. Official methods of analysis of AOAC International. 19th edn. AOAC International. Gaithersburg, MD.



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