Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

Rony Marsyal Kunda; Slamet Diah Volkandari; Maman Rumanta; Pieter Kakisina

doi:10.21059/buletinpeternak.v44i4.58934

Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

https://doi.org/10.21059/buletinpeternak.v44i4.58934

Rony Marsyal Kunda^(1*), Slamet Diah Volkandari⁽²⁾, Maman Rumanta⁽³⁾, Pieter Kakisina⁽⁴⁾

(1) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
(2) Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI) Bogor, 16911, Indonesia
(3) Department of Biology, Faculty of Teacher Training and Education, Universitas Terbuka, Tangerang Selatan, 15418, Indonesia
(4) 1Department of Biology, Faculty of Mathematics and Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
(*) Corresponding Author

Abstract

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.

Keywords

Growth hormone gene; Lakor goat; Polymorphism; Southwest Maluku Regency

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References

Akers, R. M. 2006. Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. J. Dairy Sci. 89: 1222–1234. http://dx.doi. org/10.3168/jds.S0022-0302(06)72191-9.

An, X. P., L. X. Wang, J. X. Hou, G. Li, Y. X. Song, J. G. Wang, M. M. Yang, Y. Cui, and B. Y. Cao. 2011. Novel polymorphisms of goat growth hormone and growth hormone receptor genes and their effects on growth traits. Mol. Biol. Rep. 38: 4037–4043.

Marini, A., A. B., K. Aslinda, R. M. Hifzan, A. B. M. Faisal, and K. Musaddin. 2012. HaeIII-RFLP Polymorphism of growth hormone gene in Savanna and Kalahari goats. Malaysian. J. Anim. Sci.15: 13-19.

Batubara, A., R. R. Noor, A. Farajallah, and B. Tiesnamurti. 2013. Genetic Diversity of Y-chromosomal DNA in Six Local Indonesian Goat Breeds. Proceedings IAARD Press, Indonesian. p316-325.

Bai, W. L., J. Wang, and R. Y. Yin. 2005. Study on genetic polymorphism of HaeIII site of GH gene in Chengdu-Ma goat and Boer goat. Heilongjiang. Anim. Sci. Vet. Med. 8: 13-14.

Bin, L. X., Y. X. Ling, S. H. Wang, T. M. Chao, W. T. Gu, and L. C. Zhong. 2009. Environmental influence on precision and accuracy estimating gene effect by molecular marker regression model. Inform. Comput. 2: 132-135.

Bostein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32: 314-331.

Budisatria, I. G. S., H. M. J. Udo, A. J. van der Zijpp, E. Baliarti, and T. W. Murti. 2008. Religious festivities and marketing of small ruminants in central Java-Indonesia. Asian. J. Agric. Dev. 5: 57-73.

Charlesworth, D. and J. H. Willis. 2009. The genetics of inbreeding depression. Nat. Rev. Genet. 10: 783-796.

Dekkers, J. C. M. 2004. Commercial application of marker and gene assisted selection in livestock: Strategies and lessons. J. Anim. Sci. 82: E313-E328.

Falconer, D.S. and T.F.C. Mackay. 1996. Introduction to quantitative genetics. Fourth edition. Longman Group, England.

Guo, X. and R. C. Elston. 1999. Linkage information content of polymorphic genetic markers. Hum. Hered. 49: 112-118.

Hardjosubroto, W. 2004. Aplikasi Pemuliabiakan Ternak di Lapangan. Grasindo, PT Gramedia Widiasarana Indonesia, Jakarta.

Hua, G. H, S. L. Chen, J. N. Yu, K. L. Cai, C. J. Wu, Q. L. Li, C. Y. Zhang, A. X. Liang, L. Han, L. Y. Geng, Z. Shen, D. Q. Xu, and L. G. Yang. 2009. Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat. Sci. 81: 391-395.

Ilham F., S. D. A. B. Rachman, M. I. A. Dagong, L. Rahim, and Yulianty. 2016. Genetic polymorphisms of growth hormone (GH) gene in Kacang goat population based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) identification. Livestock. Res. Rur. Dev. 28: 1-7.

Kalinowski, S. T., M. L. Taper, and T. C. Marshall. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol. Ecol. 16: 1099-1106.

Katoh, K., S. Kouno, A. Okazaki, K. Suzuki, and Y. Obara. 2008. Interaction of GH polymorphism with body weight and endocrine functions in Japanese black calves. Domest. Anim. Endocrinol. 34: 25–30.

Keller, L. F. and D. M. Waller. 2002. Inbreeding effects in wild populations. TRENDS. Ecol Evol. 17: 230-241.

Liu, Z. J. and J. F. Cordes. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture. 238: 1-37.

Mahrous, K. F., S. H. Abdel-Aziem, M. A. M. Abdel-Hafez, M. Abdel-Mordy, and H. E. Rushdi. 2018. Polymorphism of growth hormone gene in three goat breeds in Egypt. Bulletin of the National Research Centre. 42: 35.

Nei, M. and S. Kumar. 2000. Molecular evolution and phylogenetics. Oxford University Press. New York.

Rumanta, M., R. M. Kunda, S. D. Volkandari, Indriawati, and P. Kakisina. 2020. Genetic characterization and phylogenetic study of Lakor goat from Southwest Maluku Regency based on mitochondrial COI gene. Vet. World. 13: 1209-1220.

Salamena, J. F., D. Malle, C. C. E. Latupeirissa, and I. P. Siwa. 2014. Potential development of local animal genetic resources in Maluku. Occas. Pap. 54: 17-25.

Singh, P. P., S. S. Tomar, M. S. Thakur, and A. Kumar. 2015. Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat. Vet. World. 8(3):382-387.

Sodhi, M., M. Mukesh, B. Prakash, B. P. Mishra, R. C. Sobti, K. P. Singh, and S. P. S. Ahlawat. 2007. MspI allelic pattern of bovine growth hormone gene in Indian Zebu cattle (Bosindicus) breeds. Biochem. genet. 45: 145-153.

Sulastri, Siswanto, and K. Adhianto. 2017. Correlation of polymorphism of growth hormone to the pre-weaning Saburai goat growth. Proceedings of PERIPI. Pages 254-261.

Susilorini, T. E., Kuswati, and S. Maylinda. 2017. Polymorphism of Growth Hormone Gene in Selecting Etawah Crossbred (PE) Goats. Res. J. Life. Sci. 04: 153-158.

Supakorn, C. 2009. The important candidate genes in goats-A review. Walailak. J. Sci. Technol. 6: 17-36.

Seevagan, M., V. Jeichitra, R. Rajendran, and K. G. Tirumurugaan. 2015. Detection of lethal SNP (A781G) in growth hormone (GH) gene of Indian sheep. Small. Rumin. Res. 126: 13–15.

Volkandari, S. D., P. Kakisina, M. Rumanta, and R. M. Kunda. 2019. Genetic Identification of POU1F1/PstI Gene of Lakor Goat from Lakor Island, Southwest Maluku Regency. IOP Conference Series: Earth and Environmental Science, Moscow. p012123.

DOI: https://doi.org/10.21059/buletinpeternak.v44i4.58934