Toxicity Effect by Binahong (Anredera cordifolia) Leaf Extract in Histopathology and Liver Weight of Guinea Pigs (Cavia cobaya)

Dwi Wijayanti(1*), Edy Kurnianto(2), Enny Tantini Setiatin(3)

(1) Department of Animal Science, Faculty of Agriculture, Perjuangan University of Tasikmalaya, Tasikmalaya, 46115, Indonesia
(2) Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, 50275, Indonesia
(3) Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, 50275, Indonesia
(*) Corresponding Author


The aim of this research was to determine the toxic effect of Anredera cordifolia leaf extract on the Cavia cobaya liver which was evaluated by the histopathological examination of liver tissue. The materials used were 8 female guinea pigs 2.5 months old that were divided into 4 groups by simple random sampling, each treatment was given to 2 female C. cobaya. Treatments given were 0, 10, 50 and 90 mg of A. cordifolia leaf extract/head, designated as T0, T1, T2 and T3, respectively. Materials were given treatment daily as long as 10 days prepartum. All of the guinea pigs were slaughtered at day 11, and the liver were taken to examined their histopathological changes. Each of the liver tissues were processed by paraffin block-embedded and hematoxylin eosin (HE) staining method. The results of this study indicate the presence of albuminosa degeneration or mild degeneration (DH +) from group control and hydropic degeneration or moderate degeneration (DH ++) in all treatment groups and the weight of C. cobaya liver which was given an extract of A. cordifolia 50 mg/head was not significantly different from the control but was significantly different from 10 and 90 mg/head. The conclusion was Binahong's (A. cordifolia) leaves extract up to the dosage 90 mg/head had no significantly toxicity effect on the liver of guinea pigs (C. cobaya).


A. cordifolia; Guinea pigs; Liver, Toxicity

Full Text:



Adedapo, A., O. Mogbojuri, and B. Emikpe. 2009. Safety evaluations of the aqueous extract of the leaves of Moringa oleifera in rats. J. Med. Plants Res. 3: 586-591.

Ajiboye, T. O., A. K. Salau, M. T. Yakubu, A. T. Oladiji, M. A. Akanji, and J. I. Okogun. 2010. Acetaminophen perturbed redox homeostasis in Wistar rat liver: protective role of aqueous Pterocarpus osun leaf extract. Drug. Chem. Toxicol. 33: 77-87.

Al-Ashban, R. M., A. Shaaban, and R. R. Shah. 2010. Toxicity studies on Trigonella foenum-graecum L. seeds used in spices and as a traditional remedy for diabetes. Oriental Pharm. Exp. Med. 10: 66-78.

Beyene, T. 2016. Veterinary drug residues in food-animal products: its risk factors and potential effects on public health. J. Vet. Sci. Technol. 7: 1-7.

Cakir, M., A. Polat, S. Tekin, N. Vardi, E. Taslidere, and D. Z. Rumeysa. 2015. The effect of dexmedetomidine against oxidative and tubular damage induced by renal ischemia reperfusion in rats. Renal Failure. 37: 704-708.

Colle, D., L. P. Arantes, P. Gubert, S. C. A. da Luz, M. L. Athayde, and R. J. B. Teixeira. 2012. Antioxidant properties of Taraxacum officinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice. J. Med. Food. 15: 549-556.

Dollah, M. A., S. Parhizkar, L. A. Latiff, and M. H. B. Hassan. 2013. Toxicity effect of Nigella sativa on the liver function of rats. Adv. Pharm. Bulletin 3: 97-102.

Garmana, A. N., E. Y. Sukandar, and I. Fidrianny. 2016. Preliminary study of blood pressure lowering effect of Anredera cordifolia (Ten) steenis on Wistar rats. Int. J. Pharm. Pharm. Res. 8: 300-304.

Hastuti, U. S. 2013. Pengaruh berbagai dosis citrinin terhadap kerusakan struktur hepatosit mencit (Mus musculus) pada tiga zona lobulus hepar. Jurnal Kedokteran Brawijaya 22: 121-126.

Huang, B., X. Ban, J. He, J. Tong, J. Tian, and Y. Wang. 2010. Hepatoprotective and antioxidant activity of ethanolic extracts of edible lotus (Nelumbo nucifera Gaertn.) leaves. Food Chemist..120: 873-878.

Korani, M., S. M. Rezayat, K. Gilani, S. A. Bidgoli, and S. Adeli. 2011. Acute and subchronic dermal toxicity of nanosilver in guinea pig. Int. J. Nanomedicine. 6: 855-862.

Laksmitawati, D. R., A. Widyastuti, N. Karami, E. Afifah, D. D. Rihibiha, and H. Nufus. 2017. Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line. Bangladesh J. Pharmacol. 12: 35-40.

Lestari, D., E. Y. Sukandar, and I. Fidrianny. 2015. Anredera cordifolia leaves extract as anti-hyperlipidemia and endothelial fat content reducer in male Wistar rat. Int. J. Pharm. Clin. Res. 7: 435-439.

Miladiyah, I. and B. R. Prabowo. 2015. Ethanolic extract of Anredera cordifolia (Ten.) Steenis leaves improved wound healing in guinea pigs. Universa Medicina 3: 4-11.

Mushollaeni, W., N. Supartini, and E. Rusdiana. 2014. Toxicity test of alginate from Sargassum and Padina on the liver of mice. Food and Public Health 4: 204-208.

Nithianantham, K., M. Shyamala, Y. Chen, L. Y. Latha, S. L. Jothy, and S. Sasidharan. 2011. Hepatoprotective potential of Clitoria ternatea leaf extract against paracetamol induced damage in mice. Molecules 16: 10134-10145.

Olaleye, M., O. Adegboye, and A. Akindahunsi. 2006. Alchornea cordifolia extract protects wistar albino rats against acetaminophen-induced liver damage. Afr. J. Biotechnol. 5: 2439-2445.

Orbayinah, S. and A. Kartyanto. 2008. Efikasi Binahong (Anredera cordifolia (Tenore) Steenis) terhadap Kadar Alkaline Phospatase. Fakultas Kedokteran, Universitas Muhammadiyah Yogyakarta, Yogyakarta.

Pan, H., Y. Gao, and Y. Tu. 2016. Mechanisms of body weight reduction by black tea polyphenols. Molecules. 21: 1-11.

Pradana, D. A., I. D. Anggriani, and T. R. Setyaningrum. 2016. Potential of red spinach leaves ethanolic extract (Amaranthus tricolor L.) as a complementary therapy for hiperlipidemia: study in vivo of histopathologic and activity of alanin aminotransferase (ALT). Jurnal Sains Farmasi Klinis 3: 6-13.

Saba, A., A. Oyagbemi, and O. Azeez. 2010. Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Niger. J. Physiol. Sci. 25: 139–147.

Samik, A. and E. Safitri. 2017. Mycotoxin binders potential on histological of ovary mice exposed by zearalenone, Vet. World. 10: 353-357.

Shinde, A., J. Ganu and P. Naik. 2012. Effect of free radicals and antioxidants on oxidative stress. J. Dental. Allied. Sci. 1: 63-66.

Tamayanti, W. D., E. D. Rosa, R. Carlosono, R. M. Widharna, and B. D. Novita. 2014. The influence of high fructose with low magnesium diet on the liver and renal tubular cells histopathology of male wistar rats. Proceeding The International Conference Pharmaceutical Care. University of Muhammadiyah Malang April 19th 2014. 177-184.

Thompson, L. P., L. Pence, G. Pinkas, H. Song, and B. P. Telugu. 2016. Placental hypoxia during early pregnancy causes maternal hypertension and placental insufficiency in the hypoxic guinea pig model. Biol. Reprod. 95: 1-20.

Wijayanti, D., E. T. Setiatin, and E. Kurnianto. 2016. Efek ekstrak daun binahong (Anredera cordifolia (Ten) Steenis) terhadap profil darah merah pada marmut (Cavia cobaya). J. Sain Vet. 34 :75-83.

Wijayanti, D., E. T. Setiatin, and E. Kurnianto E. 2017. Study on postpartum estrus of guinea pigs (Cavia cobaya) using Anredera cordifolia leaf extract. Vet. World. 10: 375-379.

Wijayanti, D., E. T. Setiatin, and E. Kurnianto. 2018. Leucocyte profile and offspring production of guinea pig (Cavia cobaya) given Anredera cordifolia leaf extract. J. Indonesian Trop. Anim. Agric. 43: 19-25.

Yousef, M. I., S. A. Omar, M. I. El-Guendi, and L. A. Abdelmegid. 2010. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food. Chem. Toxicol. 48: 3246-3261.

Zin, S. R. M., S. Z. Omar, N. L. A. Khan, N. I. Musameh, S. Das, and N. M. Kassim. 2013. Effects of the phytoestrogen genistein on the development of the reproductive system of Sprague Dawley rats. Clinics. 68: 253-262.


Article Metrics

Abstract views : 3122 | views : 2456


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.