Physicochemical, Microbiology, and Sensory Characterization of Goat Milk Kefir in Various Incubation Time

https://doi.org/10.21059/buletinpeternak.v43i3.37217

Sulmiyati Sulmiyati(1*), Nur Saidah Said(2), Deka Uli Fahrodi(3), Ratmawati Malaka(4), Fatma Fatma(5)

(1) Faculty of Animal Science and Fishery, Universitas Sulawesi Barat, Majene, 91413, Indonesia
(2) Faculty of Animal Science and Fishery, Universitas Sulawesi Barat, Majene, 91413, Indonesia
(3) Faculty of Animal Science and Fishery, Universitas Sulawesi Barat, Majene, 91413, Indonesia
(4) Faculty of Animal Science, Universitas Hasanuddin, Makassar, 91214,
(5) Faculty of Animal Science, Universitas Hasanuddin, Makassar, 91214,
(*) Corresponding Author

Abstract


This study aimed to analyze the characteristics of goat milk kefir based on the incubation time variations on physicochemical, microbial and organoleptic. The method used was an experimental method using a Completely Randomized Design with 3 various incubation time which were for 12 hours, 18 hours and 24 hours with five replications. Parameters measured were physicochemical characteristics (pH, lactic acid concentration, and ethanol content), microbial characteristics which as the total number of lactic acid bacteria (LAB) colonies and the organoleptic characteristics were color, aroma, taste and favorability level towards goat milk kefir. Physicochemical and microbial data were analyzed by Analysis of Variance (ANOVA) while the organoleptic data were analyzed descriptively. The results showed that the incubation time gave significantly difference effect (P<0.05) on the pH, lactic acid concentration, the total number of LAB goat milk kefir and did not significant effect on the ethanol content. The organoleptic results showed that the incubation time didn’t significant effect on color and aroma, however gave significant affects on the tastes and favorability towards goat milk kefir. It can be concluded that the best incubation time was 24 hours. The physicochemical characteristics showed the pH value at 4.16±0.089; lactic acid concentration at 0.24±0.039%; ethanol content at 0.75±0.044%. The microbiology characteristics, the total number of Lactic Acid Bacteria at 1,24x107±0,008 CFU/ml. The characteristic of organoleptic color at 3.95 (white); aroma at 4.10 (kefir scent); taste at 4.25 (poor acid) and favorability at 4.15 (like).


Keywords


Characteristics; Goat milk; Kefir; Organoleptic; Physicochemical

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References

Adesokan, I. A., B. B. Odetoyinbo, Y. A. Ekanola, R. E. Avanrenren, and S. Fakorede. 2011. Roduction of Nigerian nono using lactic starter cultures. Pakistan J. Nutrit. 10: 203-207.

Afriani. 2010. Pengaruh penggunaan starter bakteri asam laktat Lactobacillus plantarum dan Lactobacillus fermentum terhadap total bakteri asam laktat, kadar asam dan nilai pH dadih susu sapi. Jurnal Ilmiah Ilmu-Ilmu Peternakan 13: 279-285.

Ali, A. A. 2011. Isolation and identification of lactic acid bacteria isolated from traditional drinking yoghurt in Khartoum State, Sudan. Current Research in Bacteriology 4: 16-22. http://doi:10.3923/crb.2011.16.22.

Arbangi, Z., T. Setyawardani, and M. Sulistyowati. 2014. Jumlah bakteri asam laktat (bal), mikroba, dan kadar air kefir susu kambing dengan konsentrasi biji kefir dan waktu fermentasi berbeda. Jurnal Ilmiah Peternakan 2: 87-93.

Arslan, S. 2015. A review: chemical, microbiological and nutritional characteristics of kefir. CyTA-Journal of Food. 13: 340-345. http://doi: 10.1080/19476337.2014.981588.

Chen, M. J., J. R. Liu, C. W. Lin, and Y. Y. Tzu. 2005. Study of the microbial and chemical properties of goat milk kefir produced by inoculation with Taiwanese Kefir grain. Asian-Aust. J. Anim. Sci. 18: 711-715.

Haryadi, Nurliana, and Sugito. 2013. Nilai pH dan jumlah bakteri asam laktat kefir susu kambing setelah difermentasi dengan penambahan gula dengan lama inkubasi yang berbeda. Jurnal Medika Veterinaria 7: 4-7.

Kesenkas, H., N. Dinkci, K. Seckin, O. Kinik, and S. Gonc. 2011. Antioxidant properties of kefir produced from different cow and soy milk mixtures. Journal of Agricultural Sciences. 17: 253-259.

Lengkey, H. A. W. and R. L. Balia. 2014. The effect starter dosage and fermentation time on pH and Lactic acid production. Biotechnology in Animal Husbandry 30: 339-347. http://doi:10.2298/BAH1402339L.

Motaghi, M., M. Mazaheri, N. Moazami, A. Farkhondeh, M. H. Fooladi, and E. M. Goltapeh. 1997. Short communication:kefir production in Iran. World Journal of Microbiology and Biotechnology 13: 589-581.

Pogacic, T., S. Sinko, S. Zamberlin, and D. Samazija. 2013. Microbiota of Kefir Grains. Mljekarstvo 63: 3-14.

Purbasari, N. A., D. R. Hantoro, and S. Wasito. 2013. Pengaruh konsentrasi biji kefir dan waktu fermentasi terhadap viskositas dan penilaian organoleptik kefir susu kambing. Jurnal Ilmiah Peternakan 1: 1021-1029.

Purnomo, H. and L. D. Muslimin. 2012. Chemical characteristics of pasteurized goat milk and goat milk kefir prepared using a different amount of Indonesian kefir grains and incubation times. International Food Research Journal. 19: 791-794.

Safitri, M. F. and A. Swarastuti. 2011. Kualitas kefir berdasarkan konsentrasi kefir grain. Jurnal Aplikasi Teknologi Pangan 2: 87-92.

Simova, E., D. Beshkova, A. Angelov, Ts. Hristozova, G. Fregova, and Z. Spasov. 2002. Lactic acid bacteria and yeasts in kefir grains and kefir made from them. J. Indust. Microbiol. Biotechnol. 28: 1-6.

Steel, R. G. D. and J. H. Torrie. 1993. Prinsip dan Prosedur Statistika: Suatu Pendekatan Biometric. Alih Bahasa: B. Sumantri. Gramedia Pustaka Utama, Jakarta.

Suriasih, K., W. R. Aryanta, G. Mahardika, and N. M. Astawa. 2012. Microbiological and chemical properties of kefir made of Bali cattle milk. Food Science and Quality Management 6: 12-22.

Swanson, K. M. J., F.F. Busta, E. H. Peterson, and M. Jonhson. 1992. Colony Count Methods: In Compendium of Methods for The Microbiology Examination of Foods. 3rd. Edited by C. Vanderzant, D.F. Splittsoesser. Compiled by the APHA Technical Commite on Microbiology Methods for Foods.

Tratnik, I., R. Bozanic, Z.Herceg, and I. D. A. Drgalic. 2006. The quality of plain and supplemented kefir from goats and cows milk. Int. J. Dairy Technol. 59: 40-46.

Witthuhn, R. C., T. Schoeman, and T. J. Britz. 2004. Isolation and characterization of the

microbial population of different South African Kefir Grains. Int. J. Dairy Technol. 57: 33-37. http://doi:10.1111/j.471-0307.2004.00126.x.

Yulianti, C. H. 2014. Uji beda kadar alkohol pada tape beras, ketan hitam dan singkong. Jurnal Teknika 6: 531-536.

Yusriyah, N. H. dan R. Agustini. 2014. Pengaruh waktu fermentasi dan konsentrasi bibit kefir terhadap mutu kefir susu sapi. UNESA Journal of Chemistry. 3: 53-57.



DOI: https://doi.org/10.21059/buletinpeternak.v43i3.37217

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