Suplemetasi Vitamin E dalam Cairan Rumen In Vitro: Analisis Parameter Fermentasi

https://doi.org/10.21059/buletinpeternak.v40i2.10752

Firman Nasiu(1*)

(1) Universitas Halu Oleo
(*) Corresponding Author

Abstract


The aim of this study was to examine the effect of vitamin E on fermentation parameters by using an in vitro fermentation system with ruminal fluid from male Bligon goats. Substrats used in fermentation process comprised of 65% of king grass, 20% of rice bran, and 15% of soybean meal, with additional 3% of capsulated crude palm oil (CCPO). Treatments of an in vitro fermentation were divided into 3 groups, those are group without vitamin E supplementation (R0), group with 200 mg per kg DM vitamin E supplementation (R1), and 400 mg vitamin E supplementation (R2). All groups were incubated for 48 hours according to Menke and Steingass method. Results showed that vitamin E supplementation at the level of 400 mg did not affect fermentation parameters (VFA, NH3, and pH) on ruminal fluid.

Keywords


Fermentations parameters; In vitro; Vitamin E



References

Abreu, A., J. E. Carulla, C. E. Lascano, T. E. Diaz, M. Kreuzer, and H. D. Hess. 2004. Effect of Sapindus saponariafruits on ruminal fermentation and duodenum nitrogen flow of sheep fed a tropical grass diet with and without legume. J. Anim. Sci. 82: 1392-1400.

Aharoni, Y., A. Orlov, A. Brosh, R. Granit, and J. Kanner. 2005. Effect of soybean oil supplementation of high forage fattening diet on fatty acid profiles in lipid depots of fattening bull calves, and their levels of blood vitamin E. Anim. Feed Sci. Tech. 119: 191-202.

Bergman, E. N., R. S. Reid, M. G. Murray, J. M. Brockway, and F. G. Whitelaw. 1965. Interconversions and production of volatile fatty acids in the sheep rumen. Biochem. J. 97: 53-58.

Channon, H. A. and G. R. Trout. 2002. Effect of tocopherol concentration on rancidity development during frozen storage on a cured and uncured processed pork product. Meat Sci. 62: 9-17.

Chuzaemi, S. dan J. V. Bruchem. 1990. Fisiologi Nutrisi Ruminansia. Universitas Brawijaya, Malang.

Dijkstra, J. 1994. Production and absorption of volatile fatty acid in the rumen. Livest. Prod. Sci. 39: 61-69.

Erfle, J. D., R. J. Boila, R. M. Teather, S. Mahadevan, and F. D. Sauer. 1982. Effect of pH on fermentation characteristics and protein degradation by rumen microorganisms in vitro. J. Dairy Sci. 8:1457-1464.

Gori, M. I. and M. A. Malana. 2010. Production of carboxymethyl cellulase from local isolate of Aspergillusspecies. Pak. J. Life Soc. Sci. 1: 1-6.

Han, H. and F. N. Owens. 1999. Degradation of tocopherol and tocopheryl acetate by ruminal contents in vitro. Anim. Sci. Res. Rep. 119-125.

Hungate, R. E. 1966. The Ruminant and It’s Microbes. Agricultural Experimental Station, University of California. Academic Press. New York, San Fransisco, London.

Karami, A., A. R. Alimon, A. Q. Sazili, Y. M. Goh, and M. Ivan. 2011. Effects of dietary antioxidants on the quality, fatty acid profile, and lipid oxidation oflongissimus muscle in Kacang goat with aging time. The American Meat Science Association.

Krause, K. M., D. K. Combs, and K. A. Beauchemin. 2002. Effects of forage particle size and grain fermentability in midlactation cows. II. Ruminal pH and chewing activity. J. Dairy Sci. 85: 1947- 1957.

Leedle, R. A., J. A. Z. Leedle, and M. D. Butine. 1993. Vitamin E is not degraded by ruminal microorganism : assessment with ruminal contents from a steer fed a high-concentrate diet. J. Anim. Sci. 71: 3442-3450.

Macit, M., V. Aksakal, E. Emsen, M. I. Aksu, M. Karaoglu, and N. Esenbuga. 2003. Effects of vitamin E supplementation on performance and meat quality traits of Morkaraman male lambs. Meat Sci. 63: 51-55.

McDiarmid, R. E., W. Majak, and K. J. Cheng. 1994. Procedure for analysis ofα-tocopherol acetate in bovine ruminal fluid. Can. J. Anim. Sci. 74: 391-392.

McDonald, P., R. A. Edwards, S. F. D. Greenhalgh, and C. A. Morgan. 2002.

Animal Nutrition. 6th ed. Pearson Education Limited.

Menke, K. H. and H. Steingass. 1988. Estimation of the energetic feed valuiuhuoe obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28:7-55.

Mouriño, F., R. Akkarawongsa, and P. J. Weimer. 2001. Initial pH as a determinant of cellulose digestion rate by mixed ruminal microorganisms in vitro. J. Dairy Sci. 84: 848-859.

Murray, R. K., D. K. Granner, P. A. Mayes, and V. W. Rodwell. 1996. Biokimia Harper (Harper’s Biochemistry) Edisi 24, diterjemahkan oleh dr. Andry Hartono, D.A.N. Penerbit Buku Kedokteran EGC, Jakarta.

Ranilla, M. J., M. D. Carro, S. Lopez, C. J. Newbold, and R. J.

Wallace. 2001. Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal microorganism in vitro. Br. J. Nutr. 86:717-724.

Russel, J. B. and D. B. Wilson. 1996. Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH? J. Dairy Sci. 8: 1503-1509.

Satter, L. D. and L. L. Slyter. 1972. Effect of ammonia concentration on ruminal microbes in vitro. J. Anim. Sci. 35: 237- 280.

Stern, M. D., A. Bach, and S. Calsamiglia. 2006. New Concepts in Protein Nutrition of Ruminants. 21st Annual Southwest Nutrition & Management Conference, February 23-24, 2006.

Sung, H. G., Y. Kobayashi, J. Chang, A. Ha, I. H. Hwang, and J. K. Ha. 2007. Low ruminal pH reduces dietary fiber digestion via reduced microbial attachment. Asian-Aust. J. Anim. Sci. 2: 200-207.

Tiven, N. C. 2011. Kajian minyak sawit kasar yang diproteksi dengan formaldehid sebagai aditif pakan untuk meningkatkan kualitas daging domba. Disertasi Universitas Gadjah Mada, Yogyakarta.

Wang, Y. and T. A. McAllister. 2002. Rumen microbes, enzymes, and feed digestion- a review. Asian-Aust. J. Anim. Sci. 11: 1659-1676.

Weatherburn, M. W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39: 971-974.

Li, Y. H., M. Ding, J. Wang, G. J. Xu, F. Zhao. A novel thermoacidophilic endoglucanase,Ba-EGA, from a new cellulose degradingbacterium, Bacillus sp. AC-1. Appl. Microbiol. Biotechnol. 70: 430-436.



DOI: https://doi.org/10.21059/buletinpeternak.v40i2.10752

Article Metrics

Abstract views : 2019 | views : 2846

Refbacks

  • There are currently no refbacks.




License URL: http://journal.ugm.ac.id/buletinpeternakan/pages/view/Publication%20Ethics%20and%20Malpractice%20Statement

Buletin Peternakan (Bulletin of Animal Science) Indexed by:

   
 
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.