.png)
.png)
* Corresponding Author
The Hepatoprotective Effect of Moringa oleifera Leaves on Male Wistar Rat Induced Streptozotocin-Nicotinamide
https://doi.org/10.22146/mot.90806 Moch Saiful Bachri(1), Laela Hayu Nurani(2*), Citra Ariani Edityaningrum(3), Muhammad Ma'ruf(4), Dwi Agustin Sulistyorini(5), Rizki Amelia(6), Sholihatil Hidayati(7), Rini Sulistyawati(8), Any Guntarti(9)
(1) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(2) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(3) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(4) Departement of Pharmacy, School of Health ISFI Banjarmasin, Banjarmasin
(5) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(6) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(7) Faculty of Health Sciences, Universitas dr. Soebandi, Jember
(8) Department of Pharmacy, Health Polytechnic of Bhakti Setya Indonesia, Yogyakarta
(9) Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta
(*) Corresponding Author
Abstract
Diabetes mellitus is a disease characterized by uncontrolled increases in blood sugar levels, which can cause complications in the liver. Disorder liver disease can be seen from the increasing SGOT, SGPT activity, and the number of necrosis cells in the liver. Moringa leaves contain the flavonoid quercetin with antidiabetic and antioxidant activity. This research aimed to determine the effect of administering the ethyl acetate fraction of Moringa leaves on SGOT activity, SGPT, and liver histopathology in rat induced by streptozotocin and nicotinamide. Moringa leaves macerated with 80% ethanol and fractionated with ethyl acetate were used. The research subjects were 30 rats divided into six groups. The rats were induced with streptozotocin at a dose of 65 mg/kgBW and nicotinamide 100 mg/kgBW for five days, then treated for ten days. Serum SGOT and SGPT activity were measured using a Microlab 300 Semi-Automated at a wavelength of 340 nm. Histopathological observations were carried out when the rats were dislocated, their livers were taken, and then preparations were made using Hematoxylin Eosin (HE) staining. Data were analyzed using the SPSS One Way ANOVA method, followed by the Tukey test with a confidence level of 95%. The results of the study showed that administration of Ethyl Acetate Fraction of Moringa oleifera (EA-MO) could reduce the levels of SGOT, SGPT, and the number of liver necrosis cells in rats induced by streptozotocin and nicotinamide.
Keywords
References
Alwaraiq, N.S. & Abdullah, A., 2014, ‘A review of flavonoid quercetin: metabolism, bioactivity and antioxidant properties’, Int. J. Pharmtech Res. 6(3), 933–941.
Bhakuni, G.S., Bedi, O., Bariwal, J., Deshmukh, R. & Kumar, P., 2016, ‘Animal models of hepatotoxicity’, Inflamm. Res. 65(1):13–24.
Erika, B.R., Marita, D. & Rini, S. 2014. ‘Aktivitas penangkapan radikal DPPH oleh fraksi n-heksan dan fraksi etil asetat daun kelor (Moringa Oleifera, Lamk)’, Media Farmasi 11(1), 1–6.
Faida, A. & Santik, Y. 2020. ‘Kejadian diabetes melitus tipe I pada usia 10-30 tahun’, HIGEIA (Journal of Public Health Research and Development) 4(1), 33–42.
Firdaus, Rimbawan, Marliyati S.A. & Roosita, K. 2016. ‘Model tikus diabetes yang diinduksi streptozotocin-sukrosa untuk pendekatan penelitian diabetes melitus gestasional’, Jurnal MKMI 12(1), 29–34.
Firdous, S.M. & Singh, A. 2016. ‘Effect of Ipomoea staphylina leaves on streptozotocin-nicotinamide induced type-II diabetes in Wistar rats’, Asian Pac. J. Health Sci. 3(3), 30–44.
Goud, B.J., Dwarakanath, V. & Swamy, B.K.C. 2015. ‘Streptozotocin-A Diabetogenic Agent in Animal Models’, Int. J. Pharm. Res. 3(1): 253–65.
Hardiningtyas, S.D., Purwaningsih, S. & Handharyani E. 2014. ‘Aktivitas antioksidan dan efek hepatoprotektif daun bakau api-api putih’, J. Pengolah. Has. Perikan. Indones. 17(1): 80–91.
Hasana, A.N., Sitasiwi, A.J. & Isdadiyanto, S. 2019. ‘Hepatosomatik indeks dan diameter hepatosit mencit (Mus Musculus L.) betina setelah paparan ekstrak etanol daun mimba (Azadirachta Indica Juss.)’, Jurnal Pro-Life 6(1): 1–12.
Hong, M., Li, S., Tan, H.Y., Wang, N., Tsao, S.W., Feng, Y. 2015. ‘Current Status of Herbal Medicines in Chronic Liver Disease Therapy: The Biological Effects, Molecular Targets and Future Prospects’, Int. J. Mol. Sci. 16(12), 28705-45.
Iorga, A., Dara, L. & Kaplowitz, N. 2017. ‘Drug-Induced Liver Injury: Cascade of Events Leading to Cell Death, Apoptosis or Necrosis’, Int. J. Mol. Sci. 18(5), 1018.
Irham, L.M. & Widyaningsih, W. 2017. ‘Aktifitas hepatoprotektif ekstrak etanol daun sidaguri (Sida Rhombifolia L.) dilihat dari rasio berat hepar, nilai SGPT-SGOT, dan histopatologi hepar pada tikus Sprague Dawley yang diinduksi CCL4’, Media Farmasi 14(1), 61–76.
Kılıçarslan, G. & Dönmez, N. 2016. ‘The effects of quercetin on antioxidant system and some blood parameters in experimental diabetic rats’, Bulletin of Environment, Pharmacology and Life Sciences (BEPLS) 5(6): 28–32.
Lindawati, N.Y. & Ma’ruf, S.H. 2020. ‘Penetapan kadar total flavonoid ekstrak etanol kacang merah (Phaseolus vulgaris L.) secara spektrofotometri visibel’, Jurnal Ilmiah Manuntung 6(1): 83–91.
Mthiyane, F.T., Dludla, P.V., Ziqubu, K., Mthembu, S.X.H., Muvhulawa, N., Hlengwa, N., Nkambule, B.B. & Mazibuko-Mbeje, S.E. 2022. ‘A review on the antidiabetic properties of Moringa oleifera Extracts: focusing on oxidative stress and inflammation as main therapeutic targets’, Front. Pharmacol. 13, 940572.
Priyanto, Y., Christijanti, W. Lisdiana & Marianti A. 2023. ‘Aktivitas antioksidan daun kelor (Moringa oleifera) pada tikus diabetik induksi aloksan’, Life Science 12(1), 97–106.
Procházková, D., Boušová, I. & Wilhelmová, N. 2011. ‘Antioxidant and prooxidant properties of flavonoids’, Fitoterapia 82(4), 513−523.
Ramadhani, N., Samudr, A.G., Pertiwi, R., Utami, C.D., Muslimah, A., Syahidah, W. & Khodijah, P.S. 2022. ‘Analisis total fenol dan flavonoid ekstrak etanol kulit batang sungkai (Peronema Canescens Jack)’, Pharmaceutical Journal of Indonesia 19(1): 66–76.
Rani, R., Dahiya, S., Dhingra, D., Dilbaghi, N., Kaushik, A., Kim, K.H. & Kumar, S. 2019. ‘Antidiabetic activity enhancement in streptozotocin + nicotinamide-induced diabetic rats through combinational polymeric nanoformulation’, Int. J. Nanomedicine. 14, 4383-4395
Roosdiana, A., Hendrawan V.F. & Wulandari, M. 2019. ‘The rice bran as therapy agent to decrease the SGOT/SGPT activities and improve the histopathology of liver in white rat (Rattus norvegicus) induced by high cholesterol diet’, IOP Conference Series Materials Science and Engineering 546, 062026.
Santoni, A., Efdi, M. & Fadhillah, N. 2023. ‘Profil fitokimia dan penentuan fenolik total, flavonoid total, dan uji aktivitas antioksidan ekstrak daun sungkai (Peronema Canescens Jack) dari daerah Kota Padang’, Jurnal Kimia Unand 12(1), 1–6.
Sharma, A. 2015. ‘Monosodium glutamate-induced oxidative kidney damage and possible mechanisms: a mini-review’, J. Biomed. Sci. 22, 93.
Sulistyawati, R., Nurani, L.H., Hidayati, S., Mursyidi, A. & Mustofa. 2017. ‘Standarisasi kualitas fraksi etil asetat daun kelor (Moringa oleifera Lamk.)’, The 6th University Research Colloquium 2017 Universitas Muhammadiyah Magelang 67–72.
Syahrin, S., Kairupan, C. & Loho, L. 2016. ‘Gambaran histopatologik hati Tikus Wistar yang diberi ekstrak daun kelor (Moringa oleifera) setelah diinduksi karbon tetraklorida (CCl4)’, eBiomedik 4(2), 1–5.
Szkudelski, T. 2012. ‘Streptozotocin-nicotinamide-induced diabetes in the rat. Characteristics of the experimental model’, Exp. Biol. Med. (Maywood) 237(5): 481–90.
Wulan, A.A.H., Sholeh, S.N. & Wigati, D. 2019. ‘Pengaruh pemberian fraksi etil asetat daun kelor (Moringa oleifera Lam.) terhadap gambaran histopatologi dan kadar SGPT dan SGOT pada tikus jantan galur Wistar yang induksi monosodium glutamat’, Media Farmasi Indonesia 14(1): 1455–1460.
DOI: https://doi.org/10.22146/mot.90806
Article Metrics
Abstract views : 27
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada