The Effects of Giving Coconut Milk on Aortic Wall Thickness of Rats (Rattus norvegicus strain Wistar) Fed with High Fat Diet (HFD)

Jauhar Firdaus(1*), Aris Prasetyo(2), Septa Surya Wahyudi(3), Yumna Rifda Haniefah(4)

(1) Department of Physiology, Faculty of Medicine, Jember University, East Java
(2) Department of Physiology, Faculty of Medicine, Jember University, East Java
(3) Department of Anatomy, Faculty of Medicine, Jember University, East Java
(4) Faculty of Medicine, Jember University, Jember, East Java
(*) Corresponding Author


Atherosclerosis is a chronic disease due to fat accumulation in the blood vessel walls. Atherosclerosis is the leading cause of cardiovascular disease. Indonesian people’s habit of consuming coconut milk is suspected of causing dyslipidemia, a risk factor for atherosclerosis, by increasing low-density lipoprotein (LDL). However, other studies suggest that coconut milk can increase high-density lipoprotein (HDL), an antioxidant that prevents atherosclerosis. Because of the different findings, this study aims to determine the effects of coconut milk administration on the aortic wall thickness of rats (Rattus norvegicus strain Wistar) fed a high-fat diet (HFD). This true experimental study used samples of 28 male Wistar rats divided into seven groups. These groups consisted of the normal group (N), the negative control group (Kn), and five treatment groups that received coconut milk at various doses, including 2, 5, 3.75, 5, 7. 5, and 10 ml/kgBW/day. The results obtained were then analyzed using SPSS. The statistical tests used are the independent t-test, one-way ANOVA, LSD post hoc, Pearson correlation test, and regression test. Based on this study, the results showed that giving HFD for 45 days could cause the aortic wall to thicken. Meanwhile, giving an HFD accompanied by coconut milk for 45 days can prevent the thickening of the aortic wall, with the maximum effective dose that can be given being 9.75 ml/kg BW/day.


aortic wall thickening; coconut milk; high-fat diet

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American Heart Association. 2020. Atherosclerosis. American Heart Association. Dallas.

Badan Penelitian dan Pengembangan Kesehatan. 2019. Laporan Nasional Riskesdas 2018. Jakarta.

Callista, O., D. Handayani, and K.P. Tritisari. 2015. Pengaruh Pemberian Tepung Daun Katuk Terhadap Penebalan Dinding Aorta Tikus Wistar. Indonesian Journal of Human Nutrition 2, no. 2: 85–90.

Cheng, Y.C., J.M. Sheen, W.L. Hu, and Y.C. Hung. 2017. Polyphenols and Oxidative Stress in Atherosclerosis-Related Ischemic Heart Disease and Stroke. Oxidative Medicine and Cellular Longevity 2017.

Ejike, C.E., O.O. Nwankwo, and I.I. Ijeh. 2010. Consumption Of Coconut Milk Did Not Increase Cardiovascular Disease Risk In Mice 0: 5–6.

Eyres, L., M.F. Eyres, A. Chisholm, and R.C. Brown. 2016. Coconut Oil Consumption and Cardiovascular Risk Factors in Humans. Nutrition Reviews 74, no. 4: 267–280.

Famurewa, A.C., and F.E. Ejezie. 2018. Polyphenols Isolated from Virgin Coconut Oil Attenuate Cadmium-Induced Dyslipidemia and Oxidative Stress Due to Their Antioxidant Properties and Potential Benefits on Cardiovascular Risk Ratios in Rats. Avicenna Journal of Phytomedicine 8, no. 1: 73–84.

Gani, Z. 2015. Gerakan Nasional SANTAN EKSKLUSIF. I. Malang: Media Nusa Creative.

Hao, W., and A. Friedman. 2014. The LDL-HDL Profile Determines the Risk of Atherosclerosis: A Mathematical Model. PLOS ONE 9, no. 3.

Herrington, W., B. Lacey, P. Sherliker, J. Armitage, and S. Lewington. 2016. Epidemiology of Atherosclerosis and the Potential to Reduce the Global Burden of Atherothrombotic Disease. Circulation Research 118, no. 4: 535–546.

Jannah, B.M., S. Isdadiyanto, and A.J. Sitasiwi. 2020. Histopathological of White Rats Aorta Induced by High-Fat Feed After Administered by Neem Leaf Ethanolic Extract. Biosantifika 12, no. 3: 370–378.

Karunasiri, A.N., M. Gunawardane, C.M. Senanayake, N. Jayathilaka, and K.N. Seneviratne. 2020. Antioxidant and Nutritional Properties of Domestic and Commercial Coconut Milk Preparations. International Journal of Food Science 2020.

Kim, H., S. Kim, S. Han, P.P. Rane, K.M. Fox, Y. Qian, and H.S. Suh. 2019. Prevalence and Incidence of Atherosclerotic Cardiovascular Disease and Its Risk Factors in Korea: A Nationwide Population-Based Study. BMC Public Health 19, no. 1112: 1–11.

Kumar, V., A. k. Abbas, and J.C. Aster. 2013. Robbins Basic Pathology. 9th ed. Canada: Elsevier Saunders.

Liu, Y., Y. Zhang, X. Zhang, Q. Xu, X. Yang, and C. Xue. 2017. Medium-Chain Fatty Acids Reduce Serum Cholesterol by Regulating the Metabolism of Bile Acid in C57BL/6J Mice. Food and Function 8, no. 1: 291–298.

McCarty, M.F., and J.J. DiNicolantonio. 2016. Lauric Acid-Rich Medium-Chain Triglycerides Can Substitute for Other Oils in Cooking Applications and May Have Limited Pathogenicity. Open Heart 3, no. 2: 1–5.

Neelakantan, N., J.Y.H. Seah, and R.M. Van Dam. 2020. The Effect of Coconut Oil Consumption on Cardiovascular Risk Factors: A Systematic Review and Meta-Analysis of Clinical Trials. Circulation: 803–814.

Di Pietro, N., M.P.A. Baldassarre, A. Cichelli, A. Pandolfi, G. Formoso, and C. Pipino. 2020. Role of Polyphenols and Carotenoids in Endothelial Dysfunction: An Overview from Classic to Innovative Biomarkers. Oxidative Medicine and Cellular Longevity 2020.

Sacks, F.M., A.H. Lichtenstein, J.H.Y. Wu, L.J. Appel, M.A. Creager, P.M. Kris-Etherton, M. Miller, et al. 2017. Dietary Fats and Cardiovascular Disease: A Presidential Advisory from the American Heart Association. Circulation 136, no. 3: e1–e23.

Schönfeld, P., and L. Wojtczak. 2016. Short- and Medium-Chain Fatty Acids in Energy Metabolism: The Cellular Perspective. Journal of Lipid Research 57, no. 6: 943–954.

Sugiyono. 2021. Metode Penelitian Kuantitatif, Kualitatif, Dan R&D. Ed. Sutopo. 2nd ed. Bandung: Alfabeta.

Supiyani, A., D. Sukmawati, N. Kusumorini, K. Santoso, and A.S. Satyaningtijas. 2021. Nilai Indeks Aterogenik Plasma (IAP) Dan Indeks Castelli (IC) Mencit Model Yang Diinduksi Minyak Trans. Jurnal MIPA 10, no. 2: 65.

Wei, T., J. Liu, D. Zhang, X. Wang, G. Li, R. Ma, G. Chen, X. Lin, and X. Guo. 2021. The Relationship Between Nutrition and Atherosclerosis. Frontiers in Bioengineering and Biotechnology 9: 1–10.

Wong, H.K., Q.C. Choo, and C.H. Chew. 2020. Coconut Milk Gavage Enhanced Fecal Bile Excretion by Modulating Hepatic Fxr Expression but Failed to Improve Fasting Serum Cholesterol Profile in C57BL/6 Mice. OCL - Oilseeds and Fats, Crops and Lipids 27.

Wulandari, N., I. Lestari, and N. Alfiani. 2017. Peningkatan Umur Simpan Produk Santan Kelapa Dengan Aplikasi Bahan Tambahan Pangan Dan Teknik Pasteurisasi. Jurnal Mutu Pangan 4, no. 1: 30–37.

Xian, T.K., N.A. Omar, L.W. Ying, A. Hamzah, S. Raj, K. Jaarin, F. Othman, and F. Hussan. 2012. Reheated Palm Oil Consumption and Risk of Atherosclerosis: Evidence at Ultrastructural Level. Evidence-Based Complementary and Alternative Medicine 2012, no. Ldl.

Zhang, H., M. Jiang, H. Hou, and Q. Li. 2021. Efficacy of Simvastatin on Carotid Atherosclerotic Plaque and Its Effects on Serum Inflammatory Factors and Cardiocerebrovascular Events in Elderly Patients. Experimental and Therapeutic Medicine 22, no. 2: 1–8.


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