Combined Antidiabetic and Antidyslipidemic Activity of Ageratum conyzoides and Gynura procumbens in Alloxan-induced Diabetic Rats

https://doi.org/10.22146/mot.83785

Yani Mulyani(1*), Nazarul Mastura(2), Salsha Rosilopya(3), Aris Suhardiman(4), Kania Fajarwati(5), Entris Sutrisno(6)

(1) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(2) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(3) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(4) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(5) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(6) Faculty of Pharmacy, Bhakti Kencana University, Bandung
(*) Corresponding Author

Abstract


Unhealthy lifestyle habits greatly increase the risks of various degenerative illnesses, for example, diabetes mellitus as well as dyslipidemia. Recent studies have shown that the pharmaceutical drugs used for the treatment of these conditions have undesirable side effects. This indicates that it is necessary to find more effective and safe alternative treatment options, particularly in medicinal plants, such as Ageratum conyzoides and Gynura procumbens. Therefore, this research examined the combined antidiabetic and antidyslipidemic activity of Ageratum conyzoides and Gynura procumbens extracts in alloxan-induced diabetic rats. The insulin sensitivity of the test animals was assessed at the beginning of the experiment using the oral glucose tolerance test (OGTT) through the administration of 3 g/kgBW glucose. Pancreatic destruction was induced with the intraperitoneal injection of a single dose of 150 mg/kgBW alloxan, and the rats were treated with ethanol extract for 14 days. High-density lipoprotein cholesterol (HDL-C), Triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) were measured using the enzymatic method. The results showed that the single extracts and their combination exhibited high antidiabetic and antidyslipidemic activity. This was indicated by a substantial reduction in fasting blood sugar, TG, TC, and LDL-C (p<0.05), and an insignificant increase in HDL-C. The activity of the combined extract was similar to the single extracts, but it was not better in decreasing TG levels. Based on these findings, 95% ethanol extract of Ageratum conyzoides, Gynura procumbens, and their combination exhibited high antidiabetic and antidyslipidemic activity in alloxan-induced rats.


Keywords


alloxan; Ageratum conyzoides; Gynura procumbens; antidiabetic; antidyslipidemic

Full Text:

PDF


References

Adelakun, S. A., Ogunlade, B., Omotoso, O. D., & Oyewo, O. O. (2018). Response of crude leaf extract of ageratum conyzoides on hormonal and biochemical assay in streptozotocin induced diabetic male wistar rats. Indian Journal of Physiology and Pharmacology, 62(4), 413–423.

Adesokan, A., Akanji, M., & Adewara, G. (2011). Evaluation of Hypoglycaemic Efficacy of Aqueous Seed Extract of Aframomum melegueta in Alloxan-induced Diabetic Rats. Sierra Leone Journal of Biomedical Research, 2(2), 91–94. https://doi.org/10.4314/sljbr.v2i2.63998

Agbafor, K. N., Onuohah, S. C., Ominyi, M. C., Orinya, O. F., & Alum. (2015). Antidiabetic, Hypolipidemic and Antiathrogenic Properties of Leaf Extracts of Ageratum conyzoides in Streptozotocin-Induced diabetic rats. Int.J.Curr.Microbiol.App.Sci, 4(11), 816–824. http://www.ijcmas.com

Al-Ishaq, R. K., Abotaleb, M., Kubatka, P., Kajo, K., & Büsselberg, D. (2019). Flavonoids and their anti-diabetic effects: Cellular mechanisms and effects to improve blood sugar levels. Biomolecules, 9(9). https://doi.org/10.3390/biom9090430

Algariri, K., Atangwho, I. J., Meng, K. Y., Asmawi, M. Z., Sadikun, A., & Murugaiyah, V. (2014). Antihyperglycaemic and toxicological evaluations of extract and fractions of gynura procumbens leaves. Tropical Life Sciences Research, 25(1), 75–93.

Anderson, S. L., Beutel, T. R., & Trujillo, J. M. (2020). Oral semaglutide in type 2 diabetes. Journal of Diabetes and Its Complications, 34(4), 107520. https://doi.org/10.1016/j.jdiacomp.2019.107520

Atawodi, S. E., Adepoju, O. A., & Nzelibe, H. C. (2017). Antihyperglycaemic and hypolipidemic effect of methanol extracts of ageratum conyzoides L (Asteraceae) in normal and diabetic rats. Tropical Journal of Pharmaceutical Research, 16(5), 989–996. https://doi.org/10.4314/tjpr.v16i5.4

Athyros, V. G., Doumas, M., Imprialos, K. P., Stavropoulos, K., Georgianou, E., Katsimardou, A., & Karagiannis, A. (2018). Diabetes and lipid metabolism. Hormones, 17(1), 61–67. https://doi.org/10.1007/s42000-018-0014-8

Chahal, R., Nanda, A., Akkol, E. K., Sobarzo‐sánchez, E., Arya, A., Kaushik, D., Dutt, R., Bhardwaj, R., Rahman, M. H., & Mittal, V. (2021). Ageratum conyzoides L. And its secondary metabolites in the management of different fungal pathogens. Molecules, 26(10). https://doi.org/10.3390/molecules26102933

Derosa, G., Maffioli, P., D’Angelo, A., Murzilli, S., Vanelli, A., Di Cesare Mannelli, E., & Ghelardini, C. (2022). Evaluation of Single and Associated Botanicals on Glucose Tolerance in Glucose-Induced Hyperglycemic Mice. Pharmadvances, 4(3), 189. https://doi.org/10.36118/pharmadvances.2022.42

Gulo A, S. P. (2021). The effect of sambung nyawa leaf extract (Gynura procumbens (Lour.) Merr.) on hemoglobin of rats induced by Escherichia coli bacteria. Indones J Chem Sci Technol 04:88–95. Articel, 04(02), 61–65.

Guo, S., Ouyang, H., Du, W., Li, J., Liu, M., Yang, S., He, M., & Feng, Y. (2021). Exploring the protective effect of: Gynura procumbens against type 2 diabetes mellitus by network pharmacology and validation in C57BL/KsJ db/db mice. Food and Function, 12(4), 1732–1744. https://doi.org/10.1039/d0fo01188f

Hidayaturrahmah, Budi Santoso, H., Aulia Rahmi, R., & Kartikasari, D. (2020). Blood glucose level of white rats ( Rattus norvegicus ) after giving catfish biscuit ( Pangasius hypothalmus ) . BIO Web of Conferences, 20, 04005. https://doi.org/10.1051/bioconf/20202004005

Hu, W., Lee, S., Pyo, J. H., & Kim, S. (2018). Hypoglycemic and Hypolipidemic Effects of Jerusalem artichoke Composites in Streptozotocin induced Diabetic Rats. Journal of Life Science, 28(6), 671–680.

Huang, X. L., Li, X. J., Qin, Q. F., Li, Y. S., Zhang, W. K., & Tang, H. Bin. (2019). Anti-inflammatory and antinociceptive effects of active ingredients in the essential oils from Gynura procumbens, a traditional medicine and a new and popular food material. Journal of Ethnopharmacology, 239(April), 111916. https://doi.org/10.1016/j.jep.2019.111916

Ighodaro OM, Adeosun AM, A. O. (2018). Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina. Medicina, 53, 365–374.

Jialal, I., & Singh, G. (2019). Management of diabetic dyslipidemia: An update. World Journal of Diabetes, 10(5), 280–290. https://doi.org/10.4239/wjd.v10.i5.280

Kemenkes RI. (2017). Farmakope Herbal Indonesia Edisi 2 (p. 561).

Michel, J., Abd Rani, N. Z., & Husain, K. (2020). A Review on the Potential Use of Medicinal Plants From Asteraceae and Lamiaceae Plant Family in Cardiovascular Diseases. Frontiers in Pharmacology, 11(June), 1–26. https://doi.org/10.3389/fphar.2020.00852

Mulyani, Y., Nabhani, S. R., & Sukmawati, I. K. (2021). Antioxidant and Antibacterial Activity Test of Bandotan ( Ageratum Conyzoides L .) Extract on Streptococcus Pyogenes Bacteria. 10(14), 81–100. https://doi.org/10.20959/wjpr202114-22294

Ojewale, A., Mada, S., Oyebadejo, S., Afodun, A., Aladeyelu, O., & Kolawole, B. (2020). Cardioprotective Activities of Ethanolic Extract Root of Ageratum conyzoides on Alloxan-Induced Cardiotoxicity in Diabetic Rats. BioMed Research International, 2020. https://doi.org/10.1155/2020/3189672

Osadebe, P., Odoh, E., & Uzor, P. (2014). Natural Products as Potential Sources of Antidiabetic Drugs. British Journal of Pharmaceutical Research, 4(17), 2075–2095. https://doi.org/10.9734/bjpr/2014/8382

Pashapoor, A., Mashhadyrafie, S., & Mortazavi, P. (2020). Ameliorative effect of Myristica fragrans (nutmeg) extract on oxidative status and histology of pancreas in alloxan induced diabetic rats. Folia Morphologica (Poland), 79(1), 113–119. https://doi.org/10.5603/FM.a2019.0052

Ratwita, W., Sukandar, E. Y., Adnyana, I. K., & Kurniati, N. F. (2019). Alpha mangostin and xanthone activity on fasting blood glucose, insulin and Langerhans islet of Langerhans in alloxan induced diabetic mice. Pharmacognosy Journal, 11(1), 64–68. https://doi.org/10.5530/pj.2019.1.12

Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834

World Health Organization. (2021). Cardiovascular Disease. Geneva, World Health Organization (Vol. 6, Issue 4).

Yadav, R., Khare, R. K., & Singhal, A. (2017). Qualitative Phytochemical Screening of Some Selected Medicinal Plants of Shivpuri District (M.P.). International Journal of Life-Sciences Scientific Research, 3(1), 844–847. https://doi.org/10.21276/ijlssr.2017.3.1.16

Yelin, A., & Kuntadi. (2019). Phytochemical identification of honey from several regions in Java and Sumbawa. AIP Conference Proceedings, 2120(July), 3–8. https://doi.org/10.1063/1.5115762

Zhou, T., Xu, X., Du, M., Zhao, T., & Wang, J. (2018). A preclinical overview of metformin for the treatment of type 2 diabetes. Biomedicine and Pharmacotherapy, 106(July), 1227–1235. https://doi.org/10.1016/j.biopha.2018.07.085



DOI: https://doi.org/10.22146/mot.83785

Article Metrics

Abstract views : 413 | views : 191

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada