In Vitro and In Vivo Antidiarrheal Activity of Dragon Fruit Peels Methanolic Extract

https://doi.org/10.22146/mot.81216

Stephanie Renata Yosefa(1), Erny Tandanu(2), William Leslie(3), Silvia Fransisca(4), Evelyn Angie(5), Djamin Kartarino(6), Refi Ikhtiari(7*)

(1) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(2) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(3) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(4) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(5) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(6) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(7) Faculty of Medicine, Dentistry, and Health Sciences, Universitas Prima Indonesia, Medan, North Sumatera
(*) Corresponding Author

Abstract


Diarrhea is a global major health problem reported by 2.5 billion cases annually. Diarrhea accounts for 9% of child death worldwide or the second leading cause of death among toddlers. Many local people have used traditional medicine for diarrhea therapy. Dragon fruit is a herbal plant with high vitamins and various nutritious compounds. Therefore, this study determined antidiarrheal activity from dragon fruit peel methanolic extract (Hylocereus polyrhizus). This study was experimental with in vitro and in vivo models. The in vitro model used disc diffusion and microdilution assay against the diarrhea-causing pathogen (Escherichia coli). Meanwhile, the in vivo model used male Wistar rats induced by Castro oil. This study showed that the minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) of dragon fruit peel extract against Escherichia coli were 50 and 100 mg/ml, respectively. The increased dose of dragon fruit peel methanol extract has increased the antidiarrheal index. The highest and lowest antidiarrheal index was found in the dragon fruit methanol extract-3 (76.56%) and dragon fruit methanol extract-1 (43.28%). The dragon fruit peel methanol extract can be proposed as antidiarrhea and antibacterial therapy against the diarrhea-causing pathogen.


Keywords


Antidiarrheal; Dragon Fruit; Peels; Methanol; Extract

Full Text:

PDF


References

Abdela, J. (2019) “Evaluation of In Vivo Antidiarrheal Activities of Hydroalcoholic Leaf Extract of Dodonaea viscosa L.(Sapindaceae) in Swiss Albino Mice,” Journal of Evidence-Based Integrative Medicine, 24, pp. 1–10. doi: 10.1177/2515690X19891952.

Astridwiyanti, A. A. B., Mahendra, A. N. and Dewi, N. W. S. (2019) “Uji efektivitas ekstrak etanol kulit buah naga merah (Hylocereus polyrhizus) terhadap Staphylococcus aureus ATCC 25923 secara in vitro,” Intisari Sains Medis, 10(3), pp. 482–486. doi: 10.15562/ism.v10i3.425.

Chiuman, L. (2019) "Protective Effect of Curcuma zedoria Against Copper in Rats' White Blood Cell," American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 59(1), pp. 142–148.

Chiuman, L. et al. (2021) "Improvement of Liver Function from Lemon Pepper Fruit Ethanol Extract in Streptozotocin-Induced Wistar Rats," in 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce). Medan, Indonesia: IEEE.

Depari, S. A. F. et al. (2021) “Uji Efektivitas Ekstrak Etanol Kulit Jeruk Sunkist (Citrus sinensis ( L .) Osbeck) Terhadap Kadar Gula Darah Tikus Wistar (Rattus norvegicus) Dengan Hiperkolestrolemia,” Biospecies, 14(1), pp. 1–9.

Gulo, K. N. et al. (2021) “Antioxidant Activity of Flavonoid Compounds in Ethanol and Ethyl Acetate Extract from Citrus Sinensis,” AIMS 2021 - International Conference on Artificial Intelligence and Mechatronics Systems. IEEE. doi: 10.1109/AIMS52415.2021.9466078.

Hendra, R. et al. (2019) “Antibacterial Activity of Red Dragon Peel (Hylocereus polyrhizus) Pigment,” Journal of Physics: Conference Series, 1351(1). doi: 10.1088/1742-6596/1351/1/012042.

Julianti, E., Rajah, K. K. and Fidrianny, I. (2017) "Antibacterial activity of ethanolic extract of Cinnamon bark, honey, and their combination effects against acne-causing bacteria," Scientia Pharmaceutica, 85(2). doi: 10.3390/scipharm85020019.

Kanon, M. Q., Fatimawati and Bodhi, W. (2012) “Uji Efektivitas Ekstrak Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) terhadap Penurunan Kadar Gula Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus L.) yang Diinduksi Sukrosa,” Pharmacon, 1(2), pp. 52–58.

Kardela, W., Fauziah, F. and Mayesri, S. (2018) “Biji Melinjo ( Gnetum gnemon L .) : Aktivitas Sebagai Antidiare,” Jurnal Farmasi Higea, 10(1), pp. 49–56.

Kementerian Kesehatan Republik Indonesia (2019) Hasil Utama Riset Kesehata Dasar (RISKESDAS) tahun 2018 [Chief Results of Basic Health Research (RISKESDAS) in 2018]. Jakarta: Kementerian Kesehatan RI. doi: 10.1088/1751-8113/44/8/085201.

Khotimah, T. K. et al. (2021) “Antibacterial Activity of Fractions from Extract Ethanolic of Hylocereus Polyrhizus Peel Against E. Coli and S. aureus,” Journal of Fundamental and Applied Pharmaceutical Science, 1(2). doi: 10.18196/jfaps.v1i2.11001.

Kifle, Z. D., Kidanu, B. B., et al. (2021) "Evaluation of in Vivo Antidiarrheal Activity of Solvent Fractions of Hagenia abyssinica (Rosaceae) in Swiss Albino Mice," Evidence-based Complementary and Alternative Medicine, 2021. doi: 10.1155/2021/8828331.

Kifle, Z. D., Atnafie, S. A., et al. (2021) "Methanolic Crude Extract of Hagenia abyssinica Possesses Significant Antidiarrheal Effect: Evidence for in Vivo Antidiarrheal Activity," Evidence-based Complementary and Alternative Medicine, 2021. doi: 10.1155/2021/9944629.

Manihuruk, F. M., Suryati, T. and Arief, I. I. (2017) "Effectiveness of the red dragon fruit (Hylocereus polyrhizus) peel extract as the colorant, antioxidant, and antimicrobial on beef sausage," Media Peternakan, 40(1), pp. 47–54. doi: 10.5398/medpet.2017.40.1.47.

Milanda, T. et al. (2021) "Antibacterial Activity of Red yeast rice Extract against Propionibacterium acnes ATCC 11827 and Methicilin-Resistant Staphylococcus aureus ATCC BAA-1683," Pharmacology and Clinical Pharmacy Research, 6(2), p. 83. doi: 10.15416/pcpr.v6i2.35062.

Mutia, M. S. (2019) "Histology Study of Liver Changes Paracetamol-Induced Wistar Rats Treated with Sunkist (Citrus sinensis L . Osbeck) Extract," American Scientific Research Journal for Engineering, Technology, and Science, 59(1), pp. 1–7.

Mutia, M. S. and Chiuman, L. (2019) “Efek Hepatoprotektif Ekstrak Etanol Citrus sinensis L . Osbeck pada Tikus yang Diinduksi Parasetamol [Hepatoprotective Effect of Citrus sinensis ( L .) Osbeck Ethanol Extract in Paracetamol-Induced Rats],” Majalah Kedokteran Bandung, 51(4), pp. 189–193.

Mutia, M. S., Ginting, C. N. and Yulizal, O. (2021) "Sunkist Peel Ethanol Extract Ameliorates Diabetic Nephropathy in Streptozotocin-Induced Diabetic Wistar Rats," Open Access Macedonian Journal of Medical Sciences, 9(A), pp. 1086–1091.

Niah, R. and Baharsyah, R. N. (2018) “Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Naga Merah Super (Hyclocereus costaricencis),” Jurnal Pharmascience, 5(1), pp. 14–21. doi: 10.20527/jps.v5i1.5781.

Pratiwi, D. I. et al. (2019) “Isolasi Senyawa Antioksidan Ekstrak Metanol Kulit Buah Naga Merah (Hylocereus polyrhizus),” Jurnal Fitofarmaka Indonesia, 6(1), pp. 340–346. doi: 10.33096/jffi.v6i1.466.

Safitri, A. T., Nur Adiratna, N. and Drajat S., I. (2020) “Uji Aktivitas Ekstrak Etanol Kulit Durian (Durio zibethinus Murr.) Terhadap Bakteri Propionibacterium acnes dan Staphylococcus aureus,” Jurnal Farmasi Udayana, 9(2), p. 66. doi: 10.24843/jfu.2020.v09.i02.p01.

Sari, L. S. (2020) “Diare Pada Anak,” Jurnal Abdimas Kesehatan Perintis, 2(1), pp. 13–16.

Siregar, B. C. et al. (2019) “Uji Efektivitas Ekstrak Akar Tanaman lauh Putiah ( Ficus racemosa L .) Terhadap Bakteri Escherichia coli dan Shigella dysenteriae Penyebab Diare Effectiveness assay of Lauh Putiah Plants Roots Extract ( Ficus racemosa L .) Against Escherichia coli and Shi,” 5(1), pp. 53–63.

Subagio, M. A. et al. (2020) "Utilization of banana ( Musa paradisiaca L.) peel as pectin source as antidiarrheal on castor oil-induced diarrheal Wistar rats model," Journal of thee Medical Sciences (Berkala Ilmu Kedokteran), 52(02), pp. 102–107. doi: 10.19106/jmedsci005202202001.

Suhartomi, . et al. (2020) "Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract," (Ichimat 2019), pp. 371–378. doi: 10.5220/0009515503710378.

Tang, W. et al. (2021) "Phenolic compounds profile and antioxidant capacity of pitahaya fruit peel from two red-skinned species (Hylocereus polyrhizus and Hylocereus undatus)," Foods, 10(1183), pp. 1–16. doi: 10.3390/foods10061183.

Widowati, W. et al. (2017) "Antioxidant and antiaging assays of Hibiscus sabdariffa extract and its compounds," Natural Product Sciences, 23(3), pp. 192–200. doi: 10.20307/nps.2017.23.3.192.

Winahyu, D. A., Candra Purnama, R. and Yevi Setiawati, M. (2019) “Uji Aktivitas Antioksidan pada Ekstrak Kulit Buah Naga Merah (Hylocereus polyrhizus) dengan Metode DPPH,” Jurnal Analis Farmasi, 4(2), pp. 117–121.

Zaidel, D. N. A. et al. (2017) "Extraction and characterisation of pectin from dragon fruit (hylocereus polyrhizus) peels," Chemical Engineering Transactions, 56, pp. 805–810. doi: 10.3303/CET1756135.



DOI: https://doi.org/10.22146/mot.81216

Article Metrics

Abstract views : 441 | views : 229

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada