Ana Mardiyaningsih(1*), Nur Ismiyati(2)

(1) D3 Diploma of Pharmacy, Poltekkes Bhakti Setya
(2) D3 Diploma of Pharmacy, Poltekkes Bhakti Setya
(*) Corresponding Author


Cervical cancer is one of the most common cancer in women. The high mortality rate indicates that chemotherapy has not overcome cancer disease. Strategies and development of cervical cancer treatment should be pursued. Avocado leaf (Persea americana Mill) is one of natural product that had antioxidant activity by scavenging radicals and cytotoxic activity. The aim of the study was to identify the cytotoxic activity on HeLa cervix cancer cells and to identify the chemical subtance groups of avocado leaves ethanol extract as chemopreventive agent. Active contents of avocado leaves was extracted by maceration with 96% ethanol. Chromatographic test were done to identify the chemical substances of avocado extract. Cytotoxic activity of avocado leaves ethanol extract was measured using MTT assay. The result of the phytochemical screening with thin layer chromatography test showed that the extract of avocado leaf contained flavonoids, alcaloids, and saponin. Avocado leaves ethanol extract inhibited HeLa cell growth with the IC50 of 360 µg/mL. Morphology of Hela cell showed that extract inhibited cell growth in dose dependent.


Persea americana Mill., Cervical cancer, HeLa cell line, cytotoxic activity, thin layer chromatography


Arisandi, Y, dan Andiriani, Y. 2009. Khasiat Berbagai Tanaman Untuk Pengobatan. Eska media, Jakarta

Asaolu, M.F., Asaolu, S.S., Fakunle, J.B., B.O. Emman, Okon , Ajayi E.O., and Togun, R.A., 2010, Evaluation of in-vitro Antioxidant Activities of Methanol Extracts of Persea americana and Cnidosculus aconitifolius, Pakistan Journal of Nutrition, 9 (11): 1074-1077

Davis, J.M., Navolonic, P.M., Weinstein, C.R., Steelman, L.S., Hu, W., Konovlepa, M., Blagosklonny, M.V., and McCubrey, J.A., 2003, Raf and Bcl-2 Induce Distinct and Common Pathway That Contribute to Breast Cancer Drug Resistance, Clin. Canc., Res., 9:1161-1170

Dewi, UT dan TR Saraswati. 2009. Efek Rebusan Daun Tapak Dara pada Dosis dan Frekuensi yang Berbeda terhadap Kerusakan danAkumulasi Glikogen pada Hepar Mencit (Mus musculus). BIOMA 11 (1) : 1-5

Ewesuedo R B and M J Ratain. 2003. Principle of Cancer Chemoterapy.Oncologic Therapies. Edited by : Everett E.V and Harvey M.G. Springer : Berlin. p: 44

Jemal, A., Siegel, R., Ward, E., Murray, T., Xu, J., Smigal, C. and Thun, M.J., 2008, Cancer statistics, Cancer J. Clin.; 56(2):106-130

Katja, D.G., Suryanto, E., dan Wehantouw, F., 2009, Potensi Daun Alpukat (Persea Americana Mill) Sebagai Sumber Antioksidan Alami, Chem. Prog. 2 (1) : 58-64

Oemiati, R., Rahajeng, E, dan , Yudi K., 2011, Prevalensi Tumor Dan Beberapa Faktor Yang Mempengaruhinya di Indonesia, Bul. Penelit. Kesehat, 39 (4) : 190 – 204

Ren, W., Qiao, Z., Wang, H., Zhu, L., and Zhang, L., 2003, Flavonoids: Promising Anticancer Agents, Medicinal Research Review, 23 (4): 519-53


Article Metrics

Abstract views : 2263 | views : 12329


  • There are currently no refbacks.

Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada