ISOLATION AND DETERMINATION OF KEMBANG SEPATU FLOWERS (Hibiscus rosa-sinensis L.) ETHANOLIC EXTRACT USING SPECTRODENSITOMETRY

https://doi.org/10.22146/tradmedj.8081

Mimiek Murrukmihadi(1*), Kurniati Pamungkas(2)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This research aims to isolate and determine relative concentration of alkaloid in Hibiscus rosa-sinensis L flowers. using spectrodensitometry. Ethanolic extract of H. rosa-sinensis flowers was made by maceration methode using etanol 70% as solvent. Fractionation of alkaloid compound was using VLC (Vaccum Liquid Chromatography) and using organic solfent mixture of methanol-ethyl acetate in variation concentration. Isolate was used as standard curve, then the concentration of H. rosa-sinensis flowers extract was determined using spectrodensitometry with silica gel as stationer phase and ethyl acetate: methanol ( 1:5 v / v) as mobile phase. The relative concentration of alkaloid was calculated by using standard curve Y = 29023.75 X + 5809.35, r = 0.996. Determination of isolate showed the relative concentration of alkaloid was 0.210 ± 0.035 % and this can be used as marker compound.


Keywords


H. rosa-sinensis flowers, compound isolation, concentration determination, alkaloid, spectrodensitometry



References

Anonim, 1985, Tanaman Obat Indonesia, Jilid I, Departemen Kesehatan Republik Indonesia, Jakarta

Cannell, R.J.P.,1998, Natural Product Isolation, Humana press, Totowa, New Jersey, 111-133, 209-229, 365

Dalimartha, S., 1999, Atlas Tumbuhan Obat Indonesia. Ungaran : Trubus Agriwidya

Eye, 2007, Memodernkan Obat Tradisional dari Tanaman, Republika, 23 November 2007 cit Anonim, 2007, Memodernkan Obat Tradisional dari Tanaman, http://www.kimia-lipi.net/index.php? pilihan=berita&id=58, 1 Juli 2009

Hostettman, K., Hostettman, M., Marston, A., 1995, Cara Kromatografi Preparatif, diterjemahkan oleh Kosasih Padmawinata, 9-10, Penerbit ITB, Bandung

Lenny, S., 2006, Senyawa Flavonoida, Fenil Propanoida dan Alkaloida, http://library. usu.ac.id/download/fmipa/06003489.pdf, 3 Agustus 2009

Mishra, N., Tandon, V.L., Gupta, R., 2012, Immunomodulation by Hibiscus rosa-sinensis: effect on the humoral and cellular immune response of Mus musculus, Pakistan Journal of Biological Science, 15 (6): 277-83

Patterson, C.A., 2006, Marker and Natural Health Products, Wellness Ewst Technology Watch, Canada

Putra, S.E.,2007, http://www.chem-is-try.org/artikel_kimia/biokimia/alkaloid_senyawa_organik_terbanyak_di_alam/, 23 Juli 2009

Supardjan, A.M., 1987. Pemisahan Tetrasiklin dan hasil uraiannya dalam sediaan Tetrasiklin secara KLT-densitometri, Laporan Penelitian, Lembaga Penelitian, UGM , Yogyakarta. 1-2, 9-10



DOI: https://doi.org/10.22146/tradmedj.8081

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