DETERMINATION OF ALKALOID CONTENT OF ETHANOLIC EXTRACT OF Hibiscus rosa-sinensis L. FLOWER

https://doi.org/10.22146/tradmedj.8043

Mimiek Murrukmihadi(1*), Subagus Wahyuono(2), Marchaban Marchaban(3), Sudibyo Martono(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Pharmacy, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Kembang sepatu flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based on Bioassay Guided fractionation, an active fraction was separated, and the fraction was identified is Alkaloid was the major compound based on TLC analysis. Viscosity value measured by viscometer was used as a Bioassay model of expectorant activity in vitro  and asetyl cysteine was used as positive control. Alkaloid content determination of the ethanolic extract was measured by TLC-Densitometric compared with standard curve of isolated alkaloid as the selected marker (Y=12.1360X+2901.4474). The alkaloid content in the ethanolic extract was determined as 2.35 ± 0.67 %. 


Keywords


alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.



References

Anonim, 1985, Tanaman Obat Indonesia, Jilid I, Departemen Kesehatan Republik Indonesia, Jakarta

Eye, 2007, Memodernkan Obat Tradisional dari Tanaman, Republika, 23 November 2007 cit Anonim, 2007, Memodernkan Obat Tradisional dari Tanaman, http://www.kimia-lipi.net/index.php?pilihan=berita&id=58, 1 Juli 2009

Murrukmihadi, M., 2009, Aktivitas Mukolitik Ekstrak etanolik dan Fraksi Aktif Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.) pada Mukus Usus Sapi secara In Vitro, Laporan Penelitian Program Hibah Penelitian Berkualitas Prima Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta

Dalimartha, S., 1999, Atlas Tumbuhan Obat Indonesia. Ungaran : Trubus Agriwidya

Anonim, 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, Cetakan Pertama, 3, 9-11, Departemen Kesehatan Republik Indonesia Direktorat Jenderal Pengawasan Obat dan Makanan Direktorat Pengawasan Obat Tradisional, Jakarta

Murrukmihadi, M., 2011, Isolasi dan Penetapan Kadar Alkaloid Dalam Ekstrak Etanolik, Fraksi Tidak larut Etil Asetat dan Fraksi Hasil VLC Dalam Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.), 2011, Disampaikan pada Kongres Ikatan Apoteker Indonesia di Manado



DOI: https://doi.org/10.22146/tradmedj.8043

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