ANTIOXIDANT SCREENING ACTIVITY OF SEVERAL INDONESIAN MEDICINAL PLANTS USING 2,2-DIFENIL 1-1 PICRYLHIDRAZYL(DPPH)

https://doi.org/10.22146/tradmedj.8018

Dewi Wulansari(1*), Chairul Chairul(2)

(1) Center of Biological Research, Indonesian Institute of Science
(2) Center of Biological Research, Indonesian Institute of Science
(*) Corresponding Author

Abstract


Antioxidant is a compound useful to prevent oxidative damage generated by free radical in human body, thus protecting body from many diseases. The aim of this research is to investigate antioxidant activity of some Indonesian medicinal plants. A total of 37 methanolic extracts of Indonesian plants have been screened for their antioxidant activity by in vitro method using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). Vitamin C was used as positive control. Of the samples investigated, 29 exhibited radical scavenging properties more than 50% at 1000 ppm. Five extracts exhibited high activities i.e. bark extracts of Sapium baccatum and Leucosyke capitellata, leaves extracts of Ardisia crispa, Glochidion cauliflorum, and Glochidion superbum.


Keywords


Antioxidant, medicinal plant, free radical, DPPH



References

Brand-Williams, W., Cuvelier, M.E., & Berset C., 1995, Use of a Free Radical Method to Evaluate Antioxidant Activity, Lebensm. Wiss. u. Technol., 28, 25-30

Chairul, S.M., Sumarny, M., dan Chairul, 2003, Aktivitas Antioksidan Ekstrak Air Daun Tempuyung (Sonchus arvensis L.) secara in vitro, Majalah Farmasi Indonesia, 14(4), 208-215

Matteo V.Di and Esposito E. 2003, Biochemical and Therapeutic Effects of Antioxidants in the Treatment of Alzheimer's Disease, Parkinson's Disease, and Amyotrophic Lateral Sclerosis. Current Drug Targets-CNS & Neurological Disorders, 2(2), 95-107(13)

Molyneux, P., 2004, The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, Songlklanakarin J. Sci. Technol., 26(2), 211-219

Rice-Evans, C., Miller, NJ., & Paganga G., 1996, Structure-Antioxidant Activity Relationships of Flavonoids and Phenolic Acids, Free Radical Biology & Medicine, 20(7), 933-956

Rohman, A., Riyanto, S., dan Utari, D., 2006, Aktivitas Antioksidan, Kandungan Fenolik Total dan Kandungan Flavonoid Total Ekstrak Etil Asetat Buah Mengkudu serta Fraksi-Fraksinya, Majalah Farmasi Indonesia, 17(3), 136-142

Touyz RM and Schiffrin EL. 2004. Reactive Oxygen Species in Vascular Biology: Implications in Hypertension. Histochem Cell Biol, 122, 339-352

Vimala, S., Adenan, M.I., Ahmad, A.R., & Shahdan, R., 2003, Nature’s Choice to Wellness Antioxidant Vegetables/Ulam, Forest Research Institute Malaysia, Kuala Lumpur, 31-33

Waddington RJ, Moseley R, and Embery G. 2008. Periodontal Disease Mechanisms: Reactive Oxygen Species: A Potential Role in The Pathogenesis of Periodontal Diseases. Oral Diseases, 6(3), 138-151



DOI: https://doi.org/10.22146/tradmedj.8018

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 ISSN 2406-9086
Faculty of Pharmacy
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