CYTOTOXICITY STUDY OF MEZZETIAPARVIFLORABECC. WOODBARK

https://doi.org/10.22146/tradmedj.7758

Mufidah Mufidah(1*), Elly Wahyudin(2), Gatot S. Lawrence(3), M. Sri Hartati Wahyuningsih(4), Mirianti A. Manggau(5)

(1) Faculty of Pharmacy, Hasanuddin University
(2) Faculty of Pharmacy, Hasanuddin University
(3) Faculty of Medicine, Hasanuddin University
(4) Faculty of Medicine, Universitas Gadjah Mada
(5) Faculty of Pharmacy, Hasanuddin University
(*) Corresponding Author

Abstract


The  cytotoxic activity  of acetone insoluble extract of MezzetiaparvifloraBecc. Wood bark was evaluated on HeLa cell line and it was compared with normal vero cell line to confirming the use of the plant as a traditional medicine for tumor. The experiment with normal vero cell line using MTT assay showed a percentage of cell  viability of  96.8%  at1000 µg of concentration which was not increase with the increase of  concentration of the extract. Whereas, the experiment on HeLa cell line showed a low cytotoxic activity with the viability percentage was found to be 87.4% at1000μg/ml. Therefore the extract was categorized as non-toxic and the next studies is necessary to explore the mechanism liable of the using  of the plant extract as anticancer traditional.


Keywords


Mezzetiaparviflora Becc., MTT, Cytotoxicity, Vero cell line, HeLa cell line

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DOI: https://doi.org/10.22146/tradmedj.7758

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