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* Corresponding Author
Effectiveness of Antibacterial Extract Bawang Suna (Allium schoenoprasum L.) against Methillicin-Resistant Staphylococcus aureus (MRSA) using Total Plate Count
https://doi.org/10.22146/mot.71730 Kania Claranisza Agustin(1), Fatmaria Fatmaria(2*), Indria Augustina(3)
(1) Medical Education Study Program, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(2) Department of Biomedical Pharmacy, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(3) Department of Parasitology, Faculty of Medicine, University of Palangka Raya, Palangka Raya, Central Kalimantan
(*) Corresponding Author
Abstract
Methicillin-Resistant Staphylococcus aureus (MRSA) infection can cause death which has caused the World Health Organization (WHO) in 2017 to issue a list of priority pathogens (one of which is MRSA) for the search for new antibiotic research. Bawang suna (Allium schoenoprasum L.) is believed to be able to inhibit the growth of the number of MRSA bacterial colonies because it contains saponins, alkaloids, flavonoids, tannins, triterpenoids, and steroids. The research was aimed to prove extract of bawang suna(Allium schoenoprasum L.) has effectiveness as an antibacterial to inhibit the growth of MRSA. True experimental design research design with cup count method using posttest only control design to compare the results of observing the number of MRSA bacterial colonies with and without treatment (control group) with serial dilution sampling technique serial dilution using a ratio of 1:5. The treatment used 1 ml an extract of bawang suna (Allium schoenoprasum L.) in the control group. 1 ml an extract of bawang suna (Allium schoenoprasum L.) gave a significant and closely related effect to inhibiting the growth of MRSA bacteria at a dilution level of 10-3 of 3288,50 + 1117,98, a dilution level of 10-4 of 379,25 + 33,75, and a 10-5 dilution rate of 42,00 + 8,68. The decrease in the number of MRSA bacterial colonies was due to the extract of bawang suna (Allium schoenoprasum L.) containing compounds: saponins, alkaloids, flavonoids, tannins, triterpenoids, and steroids that could prevent the growth of MRSA bacterial colonies. The extract of bawang suna (Allium schoenoprasum L.) as much as 1 ml using Total Plate Count (TPC) with serial dilution testing technique has a significant effect on dilutions of 10-3, 10-4, and 10-5 as an antibacterial to inhibit the growth of MRSA bacteria
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Amalia, A., Irma Sari., dan Risa Nursanty.,‘Aktivitas Antibakteri Ekstrak Etil Asetat Daun Sembung (Blumea Balsamifera (L.) Dc.) Terhadap Pertumbuhan Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA)’. Faculty of Math and Science. Syiah Kuala University. Proceedings of the 2017 Biotic National Seminar. ISBN: 978-602-60401-3-8. Banda Aceh. 2017.
Clebak, KT., Malone, MA,. ‘Skin Infections’.Prim Care.2018 Sep;45(3):433-454.[PubMed].
Darsana, I., Besung, I., Mahatmi, H.,‘Potensi Daun Binahong (Anredera Cordifolia (Tenore) Steenis) dalam Menghambat Pertumbuhan Bakteri Escherichia coli secara In Vitro’. Indonesia Medicus Veterinus. 2012.
Febianti, Z.,‘Uji In Vitro Efek Antimikroba Ekstrak Daun Kenikir (Cosmos caudatus H.B.K) terhadap Methicillin-Resistant Staphylococcus aureus (MRSA)’.Faculty of Medicine, University of Jember. Journal of Agromedicine and Medical Sciences. Volume 1. Number 2. Jember. 2015
Haddadin, A.S., Fappiano,S.A., Lipsett, P.A.,‘Review: Methicillin-Resistant Staphylococcus aureus (MRSA) in the intensive care unit’. Postgrad Med J 2002;78:385–392. Downloaded from http://pmj.bmj.com/ on April 15, 2021.
Ibrahim.,‘Uji Aktivitas Antibakteri dari Penjang Pangi pada Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA)’. Makassar : Faculty of Science and Technology. Alauddin State Islamic University Makassar.2016.
Lakhundi, S., Zhang, K.,‘Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology’. Clin. Microbiol. Rev.2018: NCBI. Accessed March 11, 2021. https://www.ncbi.nlm.nih.gov/books/NBK482221/#article-25074.r3
Larry, M., Baddour., et al. ‘Infective Endocarditis in Adults: Diagnosis, Antimicrobial Therapy, and Management of Complications A Scientific Statement for Healthcare Professionals From the American Heart Association’. Circulation. October 13, 2015.Downloaded from http://ahajournals.org by on April 15, 2021.
Maureen, U.O., Mitsan, O., Augustine, O., Akpoka., dan Osazee, E., Izevbuwa.,. 2019. ‘Methicillin-Resistant Staphylococcus aureus (MRSA) and anti-MRSA activities of extracts of some medicinal plants: A brief review’. Journal AIMS Microbiology, 5(2): 117–137. Published: 15 April 2019. https://www.aimspress.com/article/id/3602.
Meitasari,A.D.,‘Skrining Ekstrak Etanol Daun Jambu Biji (Psidium Guajava), Daun Mint (Mentha Piperita), Daun Serai (Cymbopogon Nardus), Rimpang Jahe (ZingiberOfficinale), dan Pelepah Pisang Ambon (Musa Paradisiaca) Terhadap Methicillin Resistant Staphylococcus aureus (MRSA)’. Faculty of Pharmacy. Muhammadiyah Surakarta University. Surakarta.2017.
Practicum Guide. ‘Panduan Praktikum (Online) Mikrobiologi Umum’. Microbiology Laboratory: Maulana Malik Ibrahim State Islamic University Malang. Malang. 2020.
Pebrinata,B.,‘Uji Aktivitas Anti Inplamasi Ektrak Etanol 70% Bawang Suna (Allium schoenoprasum L.) dengan Metode Denaturasi Protein’. Medical Education Study Program, Faculty of Medicine, University of Palangka Raya. Palangka Raya. 2020.
Pelczar, M.J., dan Chan, E.C.S., ‘Dasar-Dasar Mikrobiologi’.Volume I. Jakarta: UI Press. 2008.
Phyliria, R.,‘Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kalanduyung (Gauzuma ulmifolia Lam.) Terhadap Terhadap Pertumbuhan Staphylococcus aureusdengan Metode Difusi Cakram Kirby-Bauer’. Medical Education Study Program, Faculty of Medicine, University of Palangka Raya. Palangka Raya.2017.
Prabhoo, R.,Chaddha, R., et al. ‘Overview of Methicillin-ResistantStaphylococcus aureus mediated bone and joint infections in India’. Orthopedic Reviews 2019; 11:8070.
Putra, dkk.,‘Faktor Risiko Methicillin-Resistant Staphylococcus aureus pada Pasien Infeksi Kulit dan Jaringan Lunak di Ruang Rawat Inap’. University of Indonesia: Indonesian Journal of Internal Medicine. Jakarta. 2014; 1: 3-14.
Rahmawati, F.,‘Perbandingan Air Persan Buah Jeruk Nipis (Citrus Aurantifolia) dan Belimbing Wuluh (Averrohoa Bilimbi) Terhadap Jumlah Koloni Bakteri pada Ikan Nila (Areochromis Niloticus)’. Lampung: Faculty of Tarbiyah and Teacher Training. Raden Intan State Islamic University Lampung.2018.
Sartika, P.,‘Uji Aktivitas Antibakteri Ekstrak Etanol dan Fraksi Daun Kucai (Allium Schoenoprasum L.) Terhadap Bakteri Escherichia Colidan Staphylococcus Aureus’. achelor of Pharmacy Extension Program. Faculty of Pharmacy. University of Northern Sumatra. Medan. 2017.
Sinta, H.P.,‘Uji Aktivitas Antibakteri Ekstrak Etanol 96% Umbi Bawang Suna (Allium schoenoprasum L.) Terhadap Pertumbuhan Staphylococcus aureusdan Staphylococcus aureus dengan Metode Difusi Cakram Kirby-Bauer’. Medical Education Study Program, Faculty of Medicine, University of Palangka Raya. Palangka Raya.2019.
Sugiyono.,‘Metode Penelitian Pendidikan Pendekatan Kuantitatif, Kualitatif, dan R&D’. Bandung: Alfabeta. Bandung. 2013.
Swandari, P., Yadi, Y., Yuniati, Y.,‘Analisis Bioautografi Kromatografi Lapis Tipis dan Aktivitas Antibakteri Ekstrak Etanol Bawang Tiwai (Eleutherine bulbosa (mill.) urb.) Terhadap Methicillin-Resistant Staphylococcus aureus (MRSA)’. Journal of Science and Health. 2018; 1(9).
Tiara,A.H.,‘Uji Aktivitas Antibakteri Ekstrak Etanol 96% Umbi Bawang Suna (Allium schoenoprasum L.) Terhadap Pertumbuhan Shigella dysenteriaedengan Metode Dilusi Cair yang Diukur Menggunakan Spektrofotometer’. Medical Education Study Program, Faculty of Medicine, University of Palangka Raya. Palangka Raya.2019.
Tiasarah,A.,‘Uji In Vitro Antimikroba Ekstrak Umbi Bawang Batak (Allium chinenseG. Don.)TerhadapMethicillin-Resistant Staphylococcus aureus’.University of North Sumatra.Medan. 2017.
Utamy,B.C.,‘Perbandingan Uji Aktivitas Antibakteri Filtrat Aquadest Umbi Bawang Suna (Allium schoenoprasum L.) dengan Teknik Filtrasi Sederhana Terhadap Pertumbuhan Streptococcus pneumoniae dan Escherichia coli dengn metode Difusi Cakram (Kirby-Bauer)’. Medical Education Study Program, Faculty of Medicine, University of Palangka Raya. Palangka Raya.2019.
Yuwono, H., Biomed, M.,‘Pandemi Resistensi Antimikroba : Belajar dari MRSA’. 2010; (1): 2837–50.
Yuwono, H.,‘Staphylococcus aureus dan Methicilin-Resistant Staphylococcus aureus (MRSA)’.Department of Microbiology, Faculty of Medicine.Sriwijaya University of Palembang.Palembang. 2012.
World Health Organization., ‘WHO publishes list of bacteria for which new antibiotics are urgently needed’. 2017.http://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/en/
DOI: https://doi.org/10.22146/mot.71730
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