Assessment of Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Ethyl Acetate Fraction from Mangosteen (Garcinia mangostana L.,) Peels to Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis

Liza Pratiwi(1*)

(1) Department of Pharmaceutical Technology, Faculty of Medical, Tanjungpura University, Pontianak, Kalimantan Barat
(*) Corresponding Author


Mangosteen peels have antibacterial activity. SNEDDS has many advantages in developing a drug delivery system to increase the penetration of active compounds. The purpose of this study was to compare the effectiveness of the antibacterial SNEDDS of ethyl acetate fraction from mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS preparation as an antibacterial against Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis, that cause diabetic ulcers. This research began with maceration. The thick ethanol extracts were continued and fractionation was carried out with ethyl acetate solvents then was formulated into SNEDDS. The measurement of the antibacterial activity with the bacterial growth inhibition parameters of SNEDDS preparations extracted from ethyl acetate fraction of mangosteen peels was compared with ethyl acetate fraction of mangosteen peels without SNEDDS preparation. Data were analyzed using independent sample T-Test. The results showed the SNEDDS preparation of ethyl acetate fraction from mangosteen peel had activity against both types of bacteria causing diabetic ulcers, but it had no activity against Proteus mirabilis. The results of statistical analysis showed that there were significant differences in the activity of SNEDDS ethyl acetate fraction of mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS in Eschericia coli and Pseudomonas aeruginosa.


antibacterial; ethyl acetate fraction; mangosteen peels; SNEDDS

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Abidin, K.R., Suriadi & Adiningsih, B.S., 2013, ‘Inhibiting Factors for Diabetic Foot Ulcer Wound Proliferation in Type II Diabetes Mellitus Patients in Pontianak Kitamura Clinic’, Skripsi, Pontianak, Tanjungpura University.

Aiache, J.M., 1982, ‘Pharmacetics 2: Biopharmaceuticals’, Airlangga University Press, Surabaya.

Ajizah, A., 2004, ‘Sensitivity of Salmonella thypimurium to Psidium guajava L’, Journal of Bioscientiae. 1, 31-38.

Cunha, A.S., Grossior, J.L., Puisieux, F. & Seiller, M., 1997, ‘Insulin in w/o/w multiple emulsions: preparation, characterization and determination of stability towards proteases in vitro’, Journal of Microencapsulation. 14, 311–319.

Date, A.A., Desai, N., Dixit, R. & Nagarsenker, M., 2010, ‘Self-nanoemulsifying drug delivery systems: formulation insights, applications and advances’, Nanomedicine. 5, 1595–1616.

Difco, 1977. Manual of dehydrated culture media and reagents for microbiology and clinical laboratory procedures (9th ed.), Detroit Michigan, Difco Laboratories.

Griffin, B. & O'Driscoll, C., 2011, ‘Opportunities and challenges for oral delivery of hydrophobic versus hydrophilic peptide and protein-like drugs using lipid-based technologies’, Therapeutic Delivery. 2, 1633–1653.

Kapoor, V.K., Dureja, J. & Chadha, R., 2009, ‘Herbals in the control of ageing’, Drug Discovery Today. 14, 992–998.

Lina, E. P. & Sholihatul, M., 2016, ‘Risk factors for chronic complications (diabetic legs) in Type 2 Diabetes Mellitus’, The Indonesian Journal of Health Science. 7, 26-39.

Kneblock, K.A., Pauli, A., Iberl, B.,Weigland, H. & Weis, N., 1989, ‘Antibacterial and Antifungal Properties of Essential Oil Components’, Journal of Essensial Oil Research. 1, 119-128.

Manisha, J., Mitesh, P.H., Nidhi, S.K., Modi, D.J. & Vegad, M.M., 2012, ‘Spectrum of microbial flora in diabetic foot ulcer and antibiotic sensitivity pattern in tertiary care hospital in Ahmedabad’, National Journal of Medical Research. 3, 354-357.

Mohamad, N.A., Jusoh, N.A., Htike, Z.Z. & Win, S.L., 2014, ‘Bacteria identificaton from microscopic morphology: A review’, International Journal on Soft Computing, Artificial Intelligence and Applications. 3, 1-12.

Moongkarndi, P., Kosem, N., Kaslungka, S., Luanratana, O., Pongpan, N. & Neungton, N., 2004, ‘Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line’, Journal of Ethnopharmacology. 90, 161–166.

Monks, R., Clea Lerner, N., & Amelia, H., 2001, ‘Anticancer, antichemotatic and antimicrobial activities of marine sponges collected off the coast of Santa Catarina, southern Brazil’, Journal of Experimental Marine and Ecology. 281, 1-12.

Nengah, K.P., 2010, ‘Antibacterial Activity of Mangosteen (Garcinia Mangostana L.) Skin Extract and Its Active Compound Content’, Jurnal Teknologi dan Industri Pangan. 21, 1-5.

Parveen, N. & Khan, N.U., 1988, ‘Two xanthones from Garcinia mangostana’, Phytochemistry. 27, 3694-3696.

Pedraza-Chaverri, J., Cárdenas-Rodríguez, N., Orozco-Ibarra, M. & Pérez-Rojas, J.M., 2008, ‘Medicinal properties of mangosteen (Garcinia mangostana)’, Food and Chemical Toxicology, 46, 3227–3239.

Phitaktim, S., Chomnawang, M., Sirichaiwetchakoon, K., Dunkhunthod, B., Hobbs, G, & Eumkeb, G., 2016, ‘Synergism and the mechanism of action of the combination of α-mangostin isolated from Garcinia mangostana L. and oxacillin against an oxacillin-resistant Staphylococcus saprophyticus’, BMC Microbiology. 16,195.

Poeloengan, M. & Praptiwi., 2010, ‘Antibacterial Activity Test for Mangosteen (Garcinia mangostana Linn) Peels Extract’, Media Litbang Kesehatan. 10, 65-69.

Priya, V., Jainu, M., Mohan, S.K., Saraswati, P., & Gopan, C.S., 2010, ‘Antimicrobial activity of pericarp extract of garcinia mangostana linn’, International Journal of Pharmaceutical Sciences and Research. 1, 278-281.

Radji, M., 2010, ‘Microbiology Textbook: Pharmacy and Medical Student Guides’, Jakarta: Medical Book Publishers, EGC.

Sari, R. & Apridamayanti, P., 2015, ‘Identification of ESBL-producing bacteria in patients with III and IV Wagner diabetic ulcer. DIPA Research Report. Pontianak: Tanjungpura University.

Shafiq-un-Nabi, S., Shakeel, F., Talegaonkar, S., Ali, J., Baboota, S., Ahuja, A., et al., 2007, ‘Formulation development and optimization using nanoemulsion technique: a technical note’, American Association of Pharmaceutical Scientists. 8, 12–17.

Sinko, J.P., 2006, ‘Martin's Physical Pharmacy and Pharmaceutical Sciences’, 5th edition. Lippincott Williams and Wilkins, United State of America.

Subandrio, W.K., 1995, Antimicrobial Chemotherapy, Antibiotics. MIPA Faculty, Indonesia University.

Swarbrick, J., & Boylan, J.C., 1995, ‘Encyclopedia of Pharmaceutical Technology’, Marcel Dekker, New York.

Taher, M., Zakaria,T., Susanti, D. & Zakaria, Z., 2016, ‘Hypoglycaemic activity of ethanolic extract of Garcinia mangostana Linn in nomoglycaemic and streptozotocin-induced diabetic rats’, Complementary and Alternative Medicine. 16, 135.

Trease, G.E. & Evans, W.C., 1978, ‘A Textbook of Pharmacognosy’, 11th Edition, London: Bailleire Tindal.

Tjahjani, S., Widowati, W., Khiong, K., Suhendra, A. & Tjokropranoto, R., 2014, 'Antioxidant Properties of Garcinia Mangostana L (Mangosteen) Rind', Procedia Chemistry. 13, 198–203.

Weecharangsan, W., Opanasopit, P., Sukma, M., Ngawhirunpat, T., Sotanaphun, U. & Siripong, P., 2006, ‘Antioxidative and neuro protective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.)’, Journal Medical Principles and Practice. 15, 281-287.

Williams, A.C., & Barry, B,W., 2004, ‘Penetration enhancers’, Advanced Drug Delivery Reviews. 56, 603–618.


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