The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans

Hasyrul Hamzah(1), Triana Hertiani(2*), Sylvia Utami Tunjung Pratiwi(3), Titik Nuryastuti(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Yogyakarta, Indonesia
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Yogyakarta
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, Yogyakarta
(4) 2Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author


Biofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with anti-bacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level. Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13± 0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins can be a potential candidate for new antibiofilm for polymicrobial biofilm.


Mono-species biofilm; Polymicrobial biofilm; Tannin; SEM

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Andersson, S., Dalhammar, G., Land, C.J., Kuttuva Rajarao, G., 2009. Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans. Appl. Microbiol. Biotechnol. 82, 535–543.

Banin, E., Vasil, M.L., Greenberg, E.P., 2005. Iron and Pseudomonas aeruginosa Biofilm Formation. Proc. Natl. Acad. Sci. U. S. A. 102, 11076–11081.

Bjarnsholt, T., Kirketerp-Møller, K., Kristiansen, S., Phipps, R., Nielsen, A.K., Jensen, P.Ø., Høiby, N., Givskov, M., 2007. Silver against Pseudomonas aeruginosa biofilms. APMIS 115, 921–928.

Chen, M., Yu, Q., Sun, H., 2013. Novel Strategies for the preventation and Treatment of Biofilm Related Infections. Int. J. Mol. Sci. 14, 18488–18501.

Cowan, S.E., Gilbert, E., Liepmann, D., Keasling, J.D., 2000. Commensal Interactions in a Dual-Species Biofilm Exposed to Mixed Organic Compounds. Appl. Environ. Microbiol. 66, 4481–4485.

Donlan, R., Costeron, J., 2002. Biofilm: Survival Mechanism of Clinically relevant Microorganism.Clin Microbial Rev.

Donlan, R.M., 2002. Biofilms: Microbial Life on Surfaces. Emerg. Infect. Dis. 8, 881–890.

Hamzah, H., Pratiwi, S.U.T., Hertiani, T., 2018. Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm. E Coli 29, 8.

Harriott, M.M., Noverr, M.C., 2010. Ability of Candida albicans Mutants To Induce Staphylococcus aureus Vancomycin Resistance during Polymicrobial Biofilm Formation. Antimicrob. Agents Chemother. 54, 3746–3755.

Hess, D.J., Henry-Stanley, M.J., Barnes, A.M.T., Dunny, G.M., Wells, C.L., 2012. Ultrastructure of a Novel Bacterial Form Located in Staphylococcus aureus In Vitro and In Vivo Catheter-Associated Biofilms. J. Histochem. Cytochem. 60, 770–776.

Huang, W., Duan, Q., Li, F., Shao, J., Cheng, H., Wu, D., 2015. Sodium houttuyfonate and EDTA-Na2 in combination effectively inhibits Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans in vitro and in vivo. Bioorg. Med. Chem. Lett. 25, 142–147.

Leriche, V., Briandet, R., Carpentier, B., 2003. Ecology of mixed biofilms subjected daily to a chlorinated alkaline solution: spatial distribution of bacterial species suggests a protective effect of one species to another. Environ. Microbiol. 5, 64–71.

Liu H, Xu L, Zeng J. (2000). Role of Corrosion Products in Biofilms in Microbiologically Induced Corrosion of Carbon Steel. British Corr J 35, 131-135

Pierce, C.G., Uppuluri, P., Tummala, S., Lopez-Ribot, J.L., 2010. A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. J. Vis. Exp. JoVE.

Prakash, B., Veeregowda, B.M., Krishnappa, G., 2003. Biofilms: a survival strategy of bacteria. Curr. Sci. 85, 1299–1307.

Pratiwi, S.U.T., Hertiani, T., 2017. Efficacy of massoia oil in combination with some indonesian medicinal plants oils as anti-biofilm agent towards candida albicans. Int. J. Pharm. Sci. Res. 8, 13.

Yadav, A., Jha, S., n.d. To study the effect of guava leaf extract on biofilm formation in 7.

Sangetha, S., Zuraini, Z., Suryani, S., dan Sasidharan, S. (2009.) In situ TEM and SEM studies on the antimicrobial activity and prevention of Candida albicans biofilm by Cassia spectabilis extract. Micron (Oxford, England: 1993), 40, 439–443.

Qureshi, N., Annous, B.A., Ezeji, T.C., Karcher, P., dan Maddox, I.S. (2005). Biofilm Reactors for Industrial Bioconversion Processes: Employing Potential of Enhanced Reaction Rates. Microbial Cell Factories, 4, 24.


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