OPTIMIZATION OF STIRRING SPEED AND STIRRING TIME TOWARD NANOPARTICLE SIZE OF CHITOSAN-SIAM CITRUS PEEL (Citrus nobilis L.var Microcarpa) 70% ETHANOL EXTRACT

https://doi.org/10.22146/tradmedj.24302

Wintari Taurina(1*), Rafika Sari(2), Uray Cindy Hafinur(3), Sri Wahdaningsih(4), Isnindar Isnindar(5)

(1) Departement of Pharmacy, Medical Faculty, Tanjungpura University
(2) Departement of Pharmacy, Medical Faculty, Tanjungpura University
(3) Departement of Pharmacy, Medical Faculty, Tanjungpura University
(4) Departement of Pharmacy, Medical Faculty, Tanjungpura University
(5) Departement of Pharmacy, Medical Faculty, Tanjungpura University
(*) Corresponding Author

Abstract


Siam citrus peel (Citrus nobilis L. var. Microcarpa) is a plant derived from Sambas Regency, West Kalimantan Province. Bioavailability of herbal active compounds can be enhanced by formulating extract into nanoparticle. The polymer used was chitosan with crosslinker Na-TPP. Stirring speed and stirring time play an important role to produce small particle size in forming nanoparticle using ionic gelation method. Enhancement of stirring speed and stirring time could reduce particle size. Nanoparticles were prepared using ionic gelation method by mixing Na-TPP, extract and chitosan (1:1:6) with varying the stirring speed 500 rpm, 1000 rpm, 1500 rpm and stirring time 1 hrs, 2 hrs, 3 hrs. The particle size of nanoparticle was found to be 85.3 nm at 1000 rpm of stirring speed and 3 hrs of stirring times, with polidispersity index 0.287, zeta potential +32.37 mV and entrapment efficiency 87.12 %.

Keywords


siam citrus peel; nanoparticle; chitosan; ionic gelation



References

Avadi, M. 2010. Preparation and Characterization Of Insulin Nanoparticles Using Chitosan and Arabic Gum With Ionic Gelation Method. Nanomed: Nanotech, Biol Med. 6 : 58–63.

Bhardwaj, V., and Kumar, M. 2006. Chapter 9. Polymeric nanoparticles for oral durg delivery on Nanoparticle technology for drug delivery: Drug and the pharmaceutical science, Taylor dan Francis Group, New York, USA. pp. 231-262.

Calvo, P., Lopez, C., Villa-Jato., Alonso, M. 1997. Novel Hydrophilic Chitosan-Polyethylene Oxide Nanoparticles as Protein Carrier. J App Poly Sci. 63: 125-132.

Chang, R. 2005. Kimia Dasar : Konsep-konsep Inti Jilid 2. Erlangga, Jakarta.

Couvreur, P., Barratt, G., Fattal, E., Legrand, P., Vauthier, C. 2002. Nanocapsule technology: a review. Crit Rev Ther Drug Carrier Syst. 19:99–134.

Dangi, R., Shakya, S. 2013. Preparation, Optimization and Characterization of PLGA Nanoparticle. Int J of Pharm & Life Sci. 4(7): 2810-2818.

Desai, M., Labhasetwar, V., Walter, E., Levy, R., Amidon, G. 1997. The Mechanism of Uptake of Biodegradable Microparticle in Caco-2 Cells Is Size Dependent. Pharm Res. 14(11): 1568-1573.

Dewandari, K., Yuliani, S., Yasni, S. 2013. Ekstraksi dan Karakterisasi Nanopartikel Ekstrak Sirih Merah (Piper crocatum). J Pascapanen. 10(2): 58-65.

Dounighi, M., Eskandari, R., Avadi, M., Zolfagharian, H., Sedagi, M., Rezayat, M. 2012. Preparation and In Vitro Characterization of Chitosan Nanoparticles Containing Mesobuthus Eupeus Scorpion Venom as an Antigen Delivery System. J Venom Anim Toxins Incl Trop Dis. 18(1): 44-52.

Gupta, R.B and Kompella, U.B. 2006. Nanoparticle Technology for Drug Delivery. Vol 159. Taylor and Francis Group, New York, USA. pp.3.

Gupta V, Karar PK. Optimization of Process Variables for the Preparation of Chitosan-Alginate Nanoparticle. Int J Pharm Pharm Sci. 2011; 3(2): 78-80.

Martien, R., Loretz, B., Sandbichler, A.M., Bernkop-Schnűrch, A. 2008. Thiolated chitosan nanoparticles: transfection study in the Caco-2 differentiated cell culture. Nanotech. 19: 1-9.

Rahmawanty, D., Effionora, A., Anton, B. 2014. Formulasi Gel Menggunakan Ikan Haruan (Channa striatus) Sebagai Penyembuh Luka. Media Farmasi. 11(1): 29-40.

Ruby, J.J., Pandey, V.P. 2015. Chitosan Nanoparticles as a Nasal Drug Delivery for Memantine Hydrochloride. Int J Pharm Pharm Sci. 7(1): 34-37.

Sanjaya, Y.A and Surakusumah, W. 2008. Potensi Ekstrak Daun Pinus (Pinus merkusii Jungh) sebagai Bioherbisida Penghambat Perkecambahan Echinochloa colonum L. dan Amaranthus viridis. Jurnal Perennial. 4(1) : 1-5.

Septiani, I. 2012. Uji Aktivitas Antioksidan Kulit Jeruk Siam (Citrus nobilis L. var Microcarpa) dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil, Thesis, Pontianak: The University of Tanjungpura. Singh, R., Lillard, J.W. 2009. Review Nanoparticle Based Targeted Drug Delivery. Exp Mol Pathol. 86(3): 215-223.

Thatipamula, R.P., Palem, C.R., Gannu, R., Mudragada, S., Yamsani, M.R. 2011. Formulation and Invitro Characterization of Domperidone Loades Solid Lipid Nanoparticles and Nanostructured Lipid Carriers. Daru. 19(1): 23-32.

Tiyaboonchai, W. 2003. Chitosan Nanoparticles: A Promising System for Drug Delivery. Nareusan Univ J. 11(3): 51-66.

Wilcox, L.J., Borradaile, N.M., Huff, M.W. 1999. Antiatherogenic Properties of Naringenin, A citrus Flavonoid. Cardiovaskular Drug: Reviews. 17(2): 160-178.



DOI: https://doi.org/10.22146/tradmedj.24302

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