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Submitted
Oct 4, 2023
Accepted
Jun 25, 2025
Published
Oct 13, 2025
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Effect of Long-Term Zeolite-Based Storage on Spore Density and Propagule Potential of Mycorrhizal in Sorghum Plants
Corresponding Author(s) : Fatimah Nur Istiqomah
fatimahnuristiqomah2@gmail.com
Jurnal Ilmu Kehutanan,
Vol 19 No 2 (2025): September
Abstract
The research aimed to investigate the impact of storage duration (20 years versus less than one year) on the quantity of mycorrhizal spores and propagules within zeolite carrier media, using sorghum as the host plant. Spores were quantified through a sieving method and subsequent microscopic observation, while the numbers of propagules were determined through serial dilution down to 10-7. Following this, sorghum was cultivated, and root infection was evaluated. The findings indicated that mycorrhizae stored for less than one year yielded 416 spores/10 g and 620,000 propagules/g, compared to 259 spores/10 g and 170,000 propagules/g for those stored for twenty years. The identified spore types in both age groups included Acaulospora spp. 1, Acaulospora spp. 2, Glomus grape, and Glomus manihotis. This result suggested that the storage of mycorrhizae should be limited to less than one year to preserve a high number of spores and propagules.
Keywords
Most Probable Number
Acaulospora
Glomus
root infection
root staining
Istiqomah, F. N., Novanto, P. R., & Janati, R. N. (2025). Effect of Long-Term Zeolite-Based Storage on Spore Density and Propagule Potential of Mycorrhizal in Sorghum Plants. Jurnal Ilmu Kehutanan, 19(2), 149-156. https://doi.org/10.22146/jik.v19i2.10085
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References
- Abarca C, Bidondo LF, Bompadre J, Velázquez MS. 2024. Arbuscular mycorrhizal fungi in tomato tolerance to pathogens and nematodes: A comprehensive review. Scientia Horticulturae 329:112969. DOI: 10.1016/j.scienta.2024.112969
- Abdelmalik AM, Alsharani TS, Al-Qarawi AA, Ahmed AI, Aref IM. 2020. Response of growth and drought tolerance of Acacia seyal Del. seedlings to arbuscular mycorrhizal fungi. Plant, Soil and Environment 66:264–271. DOI: 10.17221/206/2020-PSE
- Akhtar O, Pandey D, Zoomi I, Singh U, Chaudhary KL, Mishra R, Pandey N. 2024. Role of arbuscular mycorrhizal fungi in heavy metals homoeostasis in plants. Journal of Plant Growth Regulation 43(11):3971–3985. DOI: 10.1007/s00344-024-11393-w
- Al-Nasser M, Al-Mansour Y, Al-Sayid N. 2024. The role of mycorrhizal fungi in forest ecosystem health. Journal of Selvicoltura Asean 1(6):271–281.
- Basri AHH. 2018. Kajian peranan mikoriza dalam bidang pertanian. Agrica Ekstensia 12(2):74–78.
- Brundrett MC, Bougher N, Dell B, Grove T, Malajczuk N. 1996. Working with mycorrhizas in forestry and agriculture. Australian Center for International Agricultural Research, Canberra.
- Halvorson HO, Ziegler NR. 1933. Application of statistics to problems in bacteriology: I. A means of determining bacterial population by the Dilution Method. Journal of Bacteriology 25(2):101–121. DOI: 10.1128/jb.25.2.101-121.1933
- Hazra F, Istiqomah FN, Firdaus ID, Fadhilla AN. 2024. Uji infektivitas dan efektivitas fungi mikoriza arbuskula dalam meningkatkan ketersediaan unsur hara P dan total mikroba pada bibit akasia (Acacia mangium). BioEksakta: Jurnal Ilmiah Biologi Unsoed 6(3):206. DOI: 10.20884/1.bioe.2024.6.3.12189
- INVAM. 2023. The international collection of (vesicular) arbuscular mycorrhizal fungi, The University of Kansas, Lawrence, United States. Available from https://invam.ku.edu/.
- Ishaq L, Tae AA, Airthur MA, Bako PO. 2021. Effect of single and mixed inoculation of arbuscular mycorrhizal fungi and phosphorus fertilizer application on corn growth in calcareous soil. Biodiversitas Journal of Biological Diversity 22(4): 1920-1926. DOI: https://doi.org/10.13057/biodiv/d220439
- Istiqomah FN, Novanto PR. 2023. Pengaruh dosis dan daya simpan mikoriza terhadap efektivitas dan infektivitas pada bibit kelapa sawit pre dan main nursery. WARTA Pusat Penelitian Kelapa Sawit 28(3):154–163. DOI: 10.22302/iopri.war.warta.v28i3.123
- Istiqomah FN, Novanto PR. 2025. Uji jumlah propagul dan jumlah spora mikoriza arbuskula menggunakan metode MPN (Most Probable Number) pada tanaman inang kelapa sawit (Elaeis guineensis Jacq.). WARTA Pusat Penelitian Kelapa Sawit 30(1):15–22. DOI: 10.22302/iopri.war.warta.v30i1.150
- Jayani FM, Faridah E, Nurjanto HH, Riniarti M. 2025. Phytoremediation of mercury contaminated soils by swietenia macrophylla inoculated with arbuscular mycorrhizal fungi (Acaulospora sp.). BIO Web of Conferences 167:03009. DOI: 10.1051/bioconf/202516703009
- Juniari K, Rai IN, Sukewijaya IM. 2020. Uji daya simpan dan efektivitas prototipe pupuk hayati mikoriza dengan media pembawa pasir vulkanik dan pasir laut. Agrotrop: Journal on Agriculture Science 10(2):165. Available from https://ojs.unud.ac.id/index.php/agrotrop/article/view/67075.
- Kabirun S. 2012. Mikoriza. Bios Majalah Ilmiah Semipopuler 5(2):1–7.
- Kirana IM, Syib’li MA, Sektiono AW. 2023. Uji nilai propagul jamur arbuskula mikoriza dan hubungannya dengan ketersediaan C-organik, P total, dan P tersedia yang diperoleh dari Hutan Kota Malabar Malang. Jurnal Hama dan Penyakit Tumbuhan 11(2):76–83. DOI: 10.21776/ub.jurnalhpt.2023.011.2.3
- Lapanjang I. 2019. Keberadaan mikoriza arbuskular pada lokasi pertanaman jarak pagar (Jatropha curcas L.) di Lembah Palu. Jurnal Sains Teknologi & Lingkungan 5(1):32–41. DOI: 10.29303/jstl.v5i1.104
- Muslimin SHH, Syib’li MA, Sektiono AW. 2023. Uji nilai propagul jamur arbuskula mikoriza indigenous tanah Hutan Cangar dan hubungannya dengan C-organik, P total, dan P tersedia tanah. Jurnal Hama dan Penyakit Tumbuhan 11(1):42–54. DOI: 10.21776/ub.jurnalhpt.2023.011.1.5
- Nugroho WA, Prasetya B. 2023. Eksplorasi mikoriza arbuskular pada beberapa sistem penggunaan lahan pertanian di Desa Ngawonggo, Kecamatan Tajinan, Kabupaten Malang. Jurnal Tanah dan Sumberdaya Lahan 10(1):25–35. DOI: 10.21776/ub.jtsl.2023.010.1.3
- Nurlaila A, Karyaningsih I, Kosasih D, Adhya I, Giwantara M, Walinda W. 2024. Perbanyakan mikoriza indigenus Taman Nasional Gunung Ciremai dengan berbagai tanaman inang. Jurnal Ilmu Pertanian Indonesia 29(1): 90-98.
- Nusantara AD, Bertham YH, Mansur I. 2012. Bekerja dengan fungi mikoriza arbuskula. Percetakan IPB, Bogor.
- Ouhaddou R, Anli M, Ben-Laouane R, Boutasknit A, Baslam M, Meddich A. 2025. The importance of the Glomus genus as a potential candidate for sustainable agriculture under arid environments: A review. International Journal of Plant Biology 16(1):32. DOI: 10.3390/ijpb16010032
- Rini MV, Andriyyani L, Arif MAS. 2020. Daya infeksi dan efektivitas fungi mikoriza arbuskular Gigaspora margarita pada tanaman jagung dengan masa simpan yang berbeda. Jurnal Agrotek Tropika 8(3):453. DOI: 10.23960/jat.v8i3.4331
- Safran, Anhar A, Fikrinda F. 2017. Eksplorasi fungi mikoriza arbuskula pada perkebunan kelapa sawit PT Lembah Bhakti di Rawa Singkil dengan kultur trapping. Jurnal Ilmiah Mahasiswa Pertanian 2(3):38–48. Available from https://jim.unsyiah.ac.id/JFP/article/view/3700.
- Setyaningsih L, Anen N, Sasongko DA, Gunawan A, Supriyanto B. 2023. Pengembangan pupuk hayati mikoriza oleh petani Hutan Cisangku, Desa Malasari, Kabupaten Bogor. Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat 9:206–215. Available from https://journal.ipb.ac.id/index.php/j-agrokreatif/article/view/43312.
- Siregar ZK, Fikrinda F, Alvisyahrin T. 2020. Pengaruh media pembawa dalam perbanyakan spora fungi mikoriza arbuskula. Jurnal Mikologi Indonesia 4. Available from https://jmi.mikoina.or.id/jmi/article/view/84.
- Sukarno N, Rahmawati C, Listiyowati S, Fadillah WN, Novera Y. 2023. Isolasi cendawan mikoriza arbuskula dari rizosfer tanaman berkayu asal Pulau Bangka dan karakteristik struktur kultur mikorizanya. Jurnal Sumberdaya Hayati 9(2):39–48. DOI: 10.29244/jsdh.9.2.39-48
- Sukmawati S, Adnyana IM, Supraptha DN, Busaifi R. 2023. Role of carrier media and types of indigenous MVA isolates on soil quality in corn plants in the dry land of West Nusa Tenggara. Jurnal Penelitian Pendidikan IPA 9(3):1512–1517. Available from http://www.jppipa.unram.ac.id/index.php/jppipa/article/view/3537.
- Wulandari AS, Hidayatullah CA. 2022. Efektivitas palmarosa (Cymbopogon martinii Roxb.) sebagai inang dalam perbanyakan spora fungi mikoriza arbuskula. Pages 85–94 Prosiding Seminar Nasional Mikoriza. Available from https://journal.ami-ri.org/index.php/semnasmikoriza/article/view/7.
- Yusril M, Burhanuddin. 2018. Komunitas fungi mikoriza arbuskula asal tanah gambut pada jeluk berbeda hasil penangkaran inang Zea mays L. dan Pueraria javanica. Jurnal Hutan Lestari 6(1):90–97.