Myrmecodia platytyrea Methanol Tuber Extract Ameliorates Hyperglycemia In STZ-Induced Diabetic Sprague-Dawley Male Rats
Keywords:
streptozotocin, antihyperglycaemic, Myrmecodia platytyrea, T2DM, STZ-induced
Abstract
Type 2 diabetes mellitus (T2DM) is one of the main non-communicable chronic diseases that has many complications that compromise the quality of life. Hence, the need to find alternatives to replace the current therapy or as an adjuvant. Tubers of Myrmecodia platytytrea (Rubiaceae) has been used traditionally as an alternative therapy for the management of cancer and other inflammatory-related disorders. The aim of this study was to investigate the potency of M. platytytrea methanolic tuber extract (MPMTE) as an antihyperglycemic agent, in vivo. :The streptozotocin (STZ)-induced diabetic rats were treated orally with MPMTE (100, 200 and 400 mg/kg) and metformin (positive control, 100 mg/kg) daily for 14 days. Blood glucose level and other biochemistry analysis were conducted including histological examination on liver, kidney and pancreas. The STZ-induced diabetic rats treated with MPMTE (200 and 400 mg/kg) had significant decreased (p<0.05) in fasting blood glucose, total cholesterol, triglycerides and low-density lipoprotein (LDL) with no significant changes in high-density lipoprotein (HDL) compared to STZ-induced untreated diabetic rats. Liver, kidney and pancreas were devoid of any damage caused by STZ. MPMTE had strong antihyperglycaemic activity and was protective against any STZ-induced organ damage. Thus, MPMTE can be further developed into an adjuvant therapy for diabetic patients.
References
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Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychno shenningsii Gilg in streptozotocin-nicotinamide type 2 diabetic rats. Iran Journal of Pharmaceutical Research, 11(1): 221-228.
Pinhas-Hamiel, O., & Zeitler, P. (2005). The global spread of type 2 diabetes mellitus in children and adolescents. Journal of Pediatrics ,146: 693-700.
Pittler, M., Abbot, N.C., Harkness, E.F., & Ernst, E. (2000). Location bias in controlled clinical trials of complementary/alternative therapies. International Journal of Epidemiology, 53 (5): 485–489. doi:10.1016/S0895-4356(99)00220-6. PMID 10812320.
Punitha, R., & Manohara, S. (2006). Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. Journal of Ethnopharmacology; 105: 39-46.
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Samatha, P., Venkateswarlu, M., & Siva, P.V. (2012). Lipid profile levels in type 2 diabetes mellitus from the tribal population of Adilabad in Andhra Pradesh, India. Journal of Clinical and Diagnostic Research, 6(4):590-592.
Selvamani. P., Latha, S., Elayaraja, K., Babu, P.S., & Gupta, J.K. (2008). Antidiabetic activity of the Ethanol Extract of Capparis sepiaria L Leaves. India Journal of Pharmaceutical Science, 70(3): 378-380.
Shaw, J.E., Sicree, R.A., & Zimmet, P.Z. (2010). Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice, 87: 4-14.
Shi, J., Hu, H., Zheng, X. Liang, Z, Wang, Y.T., Ung, C.O.L. (2019). An evaluation of randomized controlled trials on nutraceuticals containing traditional Chinese medicines for diabetes management: a systematic review. Chinese Medecine, 14: 54.doi: 10.1186/s13020-019-0276-3
Srinivasan, K. (2005). Spices as influencers of body metabolism: an overview of three decades of research. Food Research International, 38 (1): 77–86. doi:10.1016/j.foodres.2004.09.001.
Wellen, K.E., & Hotamisligil, G.S. (2005). Inflammation, stress, and diabetes. Journal of Clinical Investigation, 115(5): 1111–1119.
Witztum J.I., Fisher, M., Pietro, T., Steintracher, U.P., & Iiam, R.I. (1982). Nonenzymatic glucosylation of high-density lipoprotein accelerates its catabolism in guinea pigs. Diabetes. 31:1029–32
Zimmet, P.Z., Magliano, D.J., Herman, W.H. and Shaw, J.E. (2014). Diabetes: A 21st century challenge. Lancet Diabetes Endocrinology, 2, 56–64.
Aganotovic-Kustrin S., Morton, D.W., Mizaton, H.H., Zakaria, H. (2018). The relationship between major polyphenolic acids and stigmasterol to antioxidant activity in different extracts of Myrmecodia platytyrea. South African Journal of Botany, 115: 94-99.
Aganotovic-Kustrin, S., Morton, DW, Adam, A, Mizaton, HH, Zakaria, H (2017). High-performance thin-layer chromatographic methods in the evaluation of the antioxidant and anti-hyperglycemic activity of Myrmecodia platytyrea as a promising opportunity in diabetes treatment. Journal of Chromatography A, 1530: 192–196. https://doi.org/10.1016/j.chroma.2017.11.012.
Alomari, G., Al-Trad, B., Hamdan, S. et al. (2020). Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy. Drug Delivery and Translational Research, 10: 216–226.
Calle, M.C. & Fernandez. M.L. (2012). Inflammation and type 2 diabetes. Diabetes & Metabolism, 38(3): 183-191
Chan, J.C.N., Malik, V., & Jia, W. (2009). Diabetes in Asia: epidemiology, risk factors, and pathophysiology. Journal of the American Medical Association, 301: 2129-2140.
Chen, X., Wu, R., Kong, Y., Yang, Y., Gao, Y., Sun, D., Liu, Q., Dai, D., Lu, Z., Wang, N., Ge, S., & Wang, F. (2017). Tanshinone II attenuates renal damage in STZ-induced diabetic rats via inhibiting oxidative stress and inflammation. Oncotarget, 8(19): 31915–31922.
Cravotto, G., Boffa, L., Genzini, L. and Garella, D. (2010), Phytotherapeutics: an evaluation of the potential of 1000 plants. Journal of Clinical Pharmacy and Therapeutics, 35: 11-48.
Cree-Green, M., Triolo, Nadeau, K.J. (2013). Etiology of Insulin Resistance in Youth with Type 2 Diabetes. Current Diabetes Reports, 13(1): 81–88.
Das, A.V., Padayatti, P.S., & Paulose, C.S. (1996). Effect of leaf extract of Aegle marmelose (L.) Correa ex Roxb. on histological and ultrastructural changes in tissues of streptozotocin induced diabetic rats. Indian Journal of Experimental Biology, 34: 341- 345.
Ehses, J.A., Lacraz, G., & Giroix, M.H. (2009). IL-1 antagonism reduces hyperglycemia and tissue inflammation in the type 2 diabetic GK rat. Proceedings of the National Academy of Sciences of the United States of America, 106(33): 13998–14003, 2009.
Fox, A., Feng, W. & Asal, V. (2019). What is driving global obesity trends? Globalization or “modernization”? Global Health 15: 32 https://doi.org/10.1186/s12992-019-0457-y
Gandhi, G.R. & Sasikumar, P. (2012). Antidiabetic effect of Merremia emarginata Burm. F. in streptozotocin induced diabetic rats. Asian Pacific Journal of Tropical Biomedicine, 2(4): 281-286. doi:10.1016/S2221-1691(12)60023-9
Hollenbek, C.B., Chen, Y.D.I., Greenfield, M.S., Lardinois, C.K., & Reaven, G.M. (1986). Reduced plasma high density lipoprotein cholesterol concentrations need not increase when hyperglycemia is controlled with insulin in non insulin dependent diabetes mellitus. Journal of Clinical Endocrinology & Metabolism, 62:605–8.
Hugl, S.R., White, M.F., & Rhodes, C.J. (1998). Insulin-like growth factor I (IGF-I)- stimulated pancreatic beta-cell growth is glucose-dependent. Synergistic activation of insulin receptor substrate-mediated signal transduction pathways by glucose and IGF-I in INS-1 cells. Journal of Biological Chemistry, 273: 17771–17779.
International Diabetes Federation (2020). Retrieved from https://idf.org/aboutdiabetes/what-is-diabetes/facts-figures.html.
Institute for Public Health (2020). National Health and Morbidity Survey (NHMS) 2019: Non-communicable diseases, healthcare demand, and health literacy—Key Findings
Ishihara, K. & Hirano, T. (2002). IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine and Growth Factor Reviews, 13(4-5): 357–368.
Kulkarni, R.N. (2005). New insights into the roles of insulin/IGF-I in the development and maintenance of beta-cell mass. Reviews in Endocrine and Metabolic Disorders, 6: 199–210.
Liu, Q., Meng, X., Li, Y., Zhao, C. N., Tang, G. Y., & Li, H. B. (2017). Antibacterial and Antifungal Activities of Spices. International Journal of Molecular Sciences, 18(6), 1283. https://doi.org/10.3390/ijms18061283
Lok, A.F.S.L & Tan, H.T.W. (2009). Tuberous, epiphytic, rubiaceaous myrmecophytes of Singapore. Nature in Singapore, 2, 231-236
Misra, A., Gopalan, H., Hills, A.P., Soares, M., Reza-Albarran, Ramaiya, K.L. (2019). Diabetes in developing countries. Journal of Diabetes, 11(7): 522-539
Hasan, M.H., Zin, M.M., Wahab, I.A., Ponto, T., Adam, A. (2018). Myrmecodia platytyrea aqueous tuber extract inhibits pain. Malaysian Journal of Biochemistry and Molecular Biology, 21 (2): 23-27.
Mohammad, R.M., Afsaneh M.T., Mahmoud, B., & Mahmoud, R.K. (2016). The research and development on the antioxidants in prevention of diabetic complications. Asian Pacific Journal of Tropical Medicine, 9(9): 825-831
Oyedemi. S.O., Adewusi, E.A., Aiyegoro, O.A., & Akinpelu, D.A. (2011). Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin–induced diabetic Wistar rats. Asian Pacific Journal of Tropical Biomedicine, 1(5): 353-358.
Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychno shenningsii Gilg in streptozotocin-nicotinamide type 2 diabetic rats. Iran Journal of Pharmaceutical Research, 11(1): 221-228.
Pinhas-Hamiel, O., & Zeitler, P. (2005). The global spread of type 2 diabetes mellitus in children and adolescents. Journal of Pediatrics ,146: 693-700.
Pittler, M., Abbot, N.C., Harkness, E.F., & Ernst, E. (2000). Location bias in controlled clinical trials of complementary/alternative therapies. International Journal of Epidemiology, 53 (5): 485–489. doi:10.1016/S0895-4356(99)00220-6. PMID 10812320.
Punitha, R., & Manohara, S. (2006). Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. Journal of Ethnopharmacology; 105: 39-46.
Saisho, Y. (2016). Postprandial C-Peptide to Glucose Ratio as a Marker of β Cell Function: Implication for the Management of Type 2 Diabetes. International Journal of Molecular Sciences, 17(5), 744. doi.org/10.3390/ijms17050744
Samatha, P., Venkateswarlu, M., & Siva, P.V. (2012). Lipid profile levels in type 2 diabetes mellitus from the tribal population of Adilabad in Andhra Pradesh, India. Journal of Clinical and Diagnostic Research, 6(4):590-592.
Selvamani. P., Latha, S., Elayaraja, K., Babu, P.S., & Gupta, J.K. (2008). Antidiabetic activity of the Ethanol Extract of Capparis sepiaria L Leaves. India Journal of Pharmaceutical Science, 70(3): 378-380.
Shaw, J.E., Sicree, R.A., & Zimmet, P.Z. (2010). Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice, 87: 4-14.
Shi, J., Hu, H., Zheng, X. Liang, Z, Wang, Y.T., Ung, C.O.L. (2019). An evaluation of randomized controlled trials on nutraceuticals containing traditional Chinese medicines for diabetes management: a systematic review. Chinese Medecine, 14: 54.doi: 10.1186/s13020-019-0276-3
Srinivasan, K. (2005). Spices as influencers of body metabolism: an overview of three decades of research. Food Research International, 38 (1): 77–86. doi:10.1016/j.foodres.2004.09.001.
Wellen, K.E., & Hotamisligil, G.S. (2005). Inflammation, stress, and diabetes. Journal of Clinical Investigation, 115(5): 1111–1119.
Witztum J.I., Fisher, M., Pietro, T., Steintracher, U.P., & Iiam, R.I. (1982). Nonenzymatic glucosylation of high-density lipoprotein accelerates its catabolism in guinea pigs. Diabetes. 31:1029–32
Zimmet, P.Z., Magliano, D.J., Herman, W.H. and Shaw, J.E. (2014). Diabetes: A 21st century challenge. Lancet Diabetes Endocrinology, 2, 56–64.
Published
2021-09-29
How to Cite
Hazizul Hasan, M., Zakaria, H., Abdul Wahab, I., Ponto, T., & Adam, A. (2021). Myrmecodia platytyrea Methanol Tuber Extract Ameliorates Hyperglycemia In STZ-Induced Diabetic Sprague-Dawley Male Rats. Indonesian Journal of Pharmacy, 32(3), 338-348. https://doi.org/10.22146/ijp.880
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