LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

Muhamad Salman Fareza; Nur Amalia  Choironi; Sri Sutji  Susilowati; Melina Puspita  Rini; Vyola  Festihawa; Irfansyah Septian Nur  Fauzi; Esti Dyah  Utami; Sarmoko Sarmoko

doi:10.22146/ijp.1328

Muhamad Salman Fareza Jurusan Farmasi, Universitas Jenderal Soedirman. Purwokerto
Nur Amalia Choironi Pharmaceutical Biology Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Sri Sutji Susilowati Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Melina Puspita Rini Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Vyola Festihawa 2. Pharmaceutical Biology Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Irfansyah Septian Nur Fauzi 1. Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Esti Dyah Utami Pharmacology and Clinical Pharmacy Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Sarmoko Sarmoko Pharmacology and Clinical Pharmacy Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123

Keywords: Callophylum soulattri, stem bark, LC-MS/MS analysis, MCF-7

Abstract

Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC₅₀ values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC₅₀ values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.

References

Abbas J., Darmawan A., Syafruddin. 2011. Isolation and structure elucidation of soulatro coumarin from stem bark of Calophyllum soulattri Burm F and in vivo antiplasmodial activity by using mice infected Plasmodium berghei. Indonesian Journal of Cancer Chemoprevetion, 2(2), 242-254.
Baek DW., Park J., Lee SL., Chae YS. 2020. Ompressive effect of cisplatin monotherapy on a patient with heavily pretreated triple-negative breast cancer with poor performance. Yeungnam University Journal of Medicine, 37(3), 230-235.
Boncler M., Rozalski M., Krajewka U., Podsedek A., Watala C. 2014. Comparison of prestoblue and MTT assays of cellular viability in the assesment of anti-proliferative effects of plant extracts on human endothelial cells. Journal of Pharmacological and Toxicological Methods, 69, 9-16.
Bray F., Ferlay J., Soerjomataram I., Siegel RL., Torre LA., Jemal A. 2019. Global Cancer Statistics 2018 : GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 68(6): 394-424.
Gupta S., Gupta P. 2020. Chapter 5. The genus Calophyllum: Review of ethnomedicinal uses, phytochemistry and pharmacology, in Bioactive Natural Products in Drug Discovery, edited Singh J., Meshram V., Gupta M. Springer Nature, Singapore. pp 215-242.
Ee GCL., Mah SH., Teh SS., Rahmani M., Go R., Taufiq-Yap YH. 2011. Soulamarin, a new coumarin from stem bark of Calophyllum soulattri, Molecules, 16, 9721-9727.
Khan MR., Kihara M., Omoloso AD. 2002. Antimicrobial activity of Calophyllum soulattri. Fitoterapia, 73, 741-743.
Lim C. Hemaroopini S., Say Y., Jong VY. 2017. Cytotoxic componds from stem bark of Calophyllum soulattri. Natural Product Communications, 12(9), 1469-1471.
Mah SH., Ee GCL., Teh SS., Rahmani M., Lim YM., Go R. 2012. Phylattrin, a new cytotoxic xanthone from Calophyllum soulattri. Molecules, 17, 8303-8311.
Mah SH., Ee GCL., Teh SS. 2013. Chemistry and cytotoxic activity of essential oil from the stem bark of Calophyllum soulattri. Asian Journal of Chemistry, 25(15), 8831-8832.
Mah SH., Ee GCL., Teh SS., Sukari MA. 2015. Calophyllum inophyllum and Calophyllum soulattri source of anti-proliferative xanthines and their structure-activity relationship. Natural Product Research, 29(1), 98-101.
Nurani LH., Mahfudh N., Gandjar IG., Rahayu I. 2020. Cytotoxic potential of Arthrospira platensis extract on cervical cancer cells line Hela: Study on antiproliferative, cell cycle, apoptosis induction and anti metastatis, 31(1), 19-26.
Ohsawa T., Yukawa M., Takao C., Murayama M., Bando H. 1992. Studies on constituents of fruit body of Polyporus umbellatus and their cytotoxic activity. Chemical Pharmaceutical Bulletin, 40(1), 143-147.
Sekiya N., Hikiami H., Nakai Y., Sakakibara I. Nozaki K., Kouta K., Shimada Y., Terasawa K. 2005. Inhibitory effects of triterpenes isolated from Chuling (Polyporus umbellatus Fries) on free radical-induced lysis of red blood cells. Biological Pharmaceutical Bulletin, 28(5), 817-821.
Septiana E., Simanjuntak P. 2018. Antioxidant activity of stem bark ethanolic extracts of Calophyllum pulcherrimum, C. soulattri dan C. teysmannii. Bulletin Penelitian Tanaman Rempah dan Obat, 29(2), 59-68.
Tanjung M., Rachmadiarti F., Prameswari A., Agyani VUW., Saputri RD., Tjahjandarie TS., Syah YM. 2018. Airlanggins A-B, two new isoprenylated benzofuran-3-ones from the stem bark of Calophyllum soulattri. Natural Product Research, 32(13), 1493-1498.
Ukiya M., Akihisa T., Tokuda H., Hirano M., Oshikubo M., Nobukuni Y., Kimura Y, Tai T., Kondo S., Nishino H. 2002. Inhibition of tumor-promoting effects by poricoic acids G and H and other lanostane-type triterpenes and cytotoxic activity of poricoic acids A and G from Poria cocos. Journal of Natural Products, 65, 462-465.
WHO. 2018, Indonesia Globocan 2020 The Global Cancer Observatory, WHO, Geneva, (Online), (https://gco.iarc.fr/today/data/factsheets/populations/360-indonesia-fact-sheets.pdf, accessed 17 January 2021).