Determination of Optimal Fermentation Condition for N-acetylglucosamine Production Using Mucor circinelloides Extracellular Chitinase

https://doi.org/10.22146/jfs.45859

Yuniwaty Halim(1*), Hardoko Hardoko(2), Reinald Febryanto Pengalila(3)

(1) Jurusan Teknologi Pangan, Universitas Pelita Harapan, Banten
(2) Jurusan Teknologi Pangan, Universitas Pelita Harapan, Banten - Jurusan Teknologi Hasil Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang
(3) Jurusan Teknologi Pangan, Universitas Pelita Harapan, Banten
(*) Corresponding Author

Abstract


This research aimed to determine the best fermentation condition, consists of pH, temperature, fermentation time and substrate concentration, in N-acetylglucosamine production from shrimp shells using crude extracellular chitinase obtained from Mucor circinelloides mould. The method used was experimental method with fermentation treatment of different pH (5, 6, 7, 8 and 9) and temperature (30, 40, 50, 60, 70 and 80°C). The optimal pH and temperature of fermentation obtained was used to determine the maximum substrate concentration (0.5, 1, 1.5 and 2%) and fermentation time (2, 4, 6 and 24 hours) to produce the highest concentration of N-acetylglucosamine. The optimal pH for fermentation was 8, with chitinase activity of 4.38±0.06 U/ml, while the optimal temperature was 50°C with enzyme activity of 5.42±0.06 U/ml. Substrate concentration and fermentation time affected the N-acetylglucosamine production. The optimal fermentation condition was obtained with substrate concentration of 1.5% and fermentation time of 2 hours resulted to N-acetyl Glucosamine concentration of 2195.83±15.14 ppm.


Keywords


Shrimp shells; chitinase; chitin; Mucor circinelloides; N-acetylglucosamine

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References

Al-Hassan, A.A. 2016. Utilization of waste: extraction and characterization of chitosan. Civil and Environmental Research. 8 (3): 117-123

Annamalai, N., V.R. Mayavan, V. Shanmugam & B. Thangavel. 2011. Purification and characterization of chitinase from Alcaligenes faecalis AUO2 by utilizing marine waste and its antioxidant activity. Annals of Microbiology. 61: 801-807

AOAC. 2005. Determination of Moisture, Ash, Protein and Fat. Official Method of Analysis of the Association of Analytical Chemists. 18th Edition, AOAC, Washington DC

Arif, A.R., H.N. Ischaida & S. Dali. 2013. Isolasi kitin dari limbah udang putih (Penaeus merguiensis) secara enzimatis. Prosiding Seminar Nasional Kimia “Peran Sains dan Teknologi dalam Mendukung Ketahanan Pangan dan Energi Nasional”. Universitas Hasanuddin, Makassar, Indonesia. 10-16

Brzezinska, M.S., U. Jankiewicz & K. Lisiecki. 2013. Optimization of cultural conditions for the production of antifungal chitinase by Streptomyces sprovirgulis. Applied Biochemistry and Microbiology. 49 (2): 154-159

Cahyani, L. 2013. Pemanfaatan Tepung Cangkang Udang sebagai Media Produksi Kitinase oleh Bakteri Kitinolitik Isolat 26. Skripsi, Universitas Jember, Jember

Cahyono, E., S. Pipih & W. Ietje. 2014 Development of a pressurized hydrolysis method for producing glucosamine. Asian Journal of Agriculture and Food Sciences. 2 (5): 390-396

Chen, J., C. Shen & C. Lui. 2010. N-acetylglucosamine: production and applications. Marine Drugs. 8 (9): 2493-2516

Czechowska-Biskup, R., J. Diana, R. Bozena, U. Piotr & M.R. Janusz. 2012. Determination of degree of deacetylation of chitosan-comparison methods. Progress on Chemistry and Application of Chitin and Its Derivative. 17: 5-20

Ghanem, K. M, S.M. Al-Garni & N.H. Al-Makishah. 2010. Statistical optimization of cultural conditions for chitinase production from fish scales waste by Aspergillus terreus. African Journal of Biotechnology. 9 (32):  5135-5146

Herdyastuti, N & S. E. Cahyaningrum. 2017. Analysis of N-acetyl-glucosamine from enzymatic degradation of amorphous chitin. Rasayan Journal of Chemistry. 10 (1): 226-233

Hossain, M.S & A. Iqbal. 2014. Production and characterization of chitosan from shrimp waste. Journal of the Bangladesh Agricultural University. 12 (1): 153-160

Islam, S. Z., M. Khan & A. Alam. Production of chitin and chitosan from shrimp shell wastes Journal of the Bangladesh Agricultural University. 14 (2): 253-259

Jamialahmadi, K., J. Behravan, M.F. Najafi, M.T. Yazdi, A.R. Shahverdi & M.A. Faramarzi. 2011. Enzymatic production of N-acetyl-D-glucosamine from chitin using crude enzyme preparation of Aeromonas sp. PTCC1691. Biotechnology. 10 (3): 292-297

Jenifer, S., J. Jeyasree, D.L. Kezia & K. Manikandan. 2014. Purification and characterization of chitinase from Trichoderma viride N9 and its antifungal activity against phytopathogenic fungi. World Journal of Pharmacy and Pharmaceutical Sciences. 3 (2): 1604-1611

Junianto, B.W & S. Siswa. 2013. Selection of methods for microbiological extraction of chitin from shrimp shells. Microbiology Indonesia. 7 (2): 75-83

McNeil, B., A. David, G. Ioannis & H. Linda. 2013. Microbial Production of Food Ingredients, Enzyme and Nutraceuticals. Woodhead Publishing. Cambridge. 494-530

Mishra, P., P. Kshirsagar, S.S. Nilegaonkar & S.K. Singh. 2012. Statistical optimization of medium components for production of extracellular chitinase by Basidiobolus ranarum: a novel biocontrol agent against plant pathogenic fungi. Journal of Basic Microbiology. 52 (5): 539-548

Nielsen, S. S. 2010. Food Analysis 4th ed. Springer Science+Business Media. New York. 578-582

Percot, A., V. Christope & D. Alain. 2003. Optimization of chitin extraction from shrimp shells. Biomacromolecules. 4 (1): 12-18

Puspawati, N.M & I.N. Simpen, 2010. Optimasi deasetilasi khitin dari kulit udang dan cangkang kepiting limbah restoran seafood menjadi khitosan melalui variasi konsentrasi NaOH. Jurnal Kimia. 4 (1): 79-90

Rahmansyah, M & I.M. Sudiana. 2003. Optimasi analisis amilase dan glukanase yang diekstrak dari miselium Pleurotus ostreatus dengan asam 3,5 dinitrosalisilat. Berkala Penelitian Hayati. 9: 7-12

Ramadhan, L.O.A.N., C.L. Radiman, D. Wahyuningrum, V. Suendo, L. O. Ahmad & S. Valiyaveetiil. 2010. Deasetilasi kitin secara bertahap dan pengaruhnya terhadap derajat deasetilasi serta massa molekul kitosan. Jurnal Kimia Indonesia 5 (1): 17-21

Salazar, J., L. Bello, M. Chávez, R. Añez, J. Rojas & V. Bermúd. 2014. Glucosamine for osteoarthritis: biological effects, clinical efficacy, and safety on glucose metabolism. Arthritis: 1-13

Sanusi, M. 2004. Transformasi kitin dari hasil isolasi limbah industri udang beku menjadi kitosan. Marina Chimca Acta. 5 (2): 28-32

Scanlon, M.G., A.W. Henrich & J.R. Whitaker. 2018. Factors Affecting Enzyme Activity in Food Processing. Proteins in Food Processing. Elsevier BV. Amsterdam. 337-365

Setia, I.N. 2015. Chinolytic assay and identification of bacteria isolated from shrimp waste based on 16S rDNA sequences. Advances in Microbiology. 5 (7): 541-548

Suryadi, Y., T.P. Priyatno,  I.M. Samudera, D.N. Susilowati, N. Lawati & E. Kustaman. 2013. Pemurnian parsial dan karakterisasi kitinase asal jamur entomopatogen Beauveria bassiana Isolat BB200109. Jurnal AgroBiogen. 9 (2):77-84

Suzuki, S., E. Nakanishi., T. Ohira, R. Kawachi, H. Nagasawa & S.Sakuda. 2006. Chitinase inhibitor allosamidin is a signal molecule for chitinase production in its producing Streptomyces. The Journal of Antibiotics. 59 (7): 402-409

Traving S.J., U.H. Thygesen, L. Riemann, C.A. Stedmon. 2015. A model of extracellular enzymes in free-living microbes: which strategy pays off?. Applied and Environmental Microbiology. 81: 7385-7393

Umemoto, N., K. Yuka, O. Takayuki, O. Takuo, N. Tomoyuki, S. Sakuda, T. Taira & T. Fukamizo.2015.Crystal structures and inhibitor binding properties of plantclass V chitinases: the cycad enzyme exhibits uniquestructural and functional features. The Plant Journal. 82: 54-66

Veronica. 2017. Uji Aktivitas Kitinolitik Kapang yang Diisolasi dari Kulit Udang Windu (Penaues monodon). Skripsi, Universitas Pelita Harapan, TangerangYounes, I & M. Rinaudo. 2015. Chitin and chitosan preparation from marine sources. structure, properties and applications. Marine Drugs. 13 (3): 1133-1174

Zhang, L.Y., X.Y. Zhang, W. Zhang, C. Lin & Y.G. Zhang. 2017. Screening and identification of chitinase producing strain and its inhibition action to dominant moulds in corn stalk. Chinese Journal of Animal Nutrition. 3: 1-8



DOI: https://doi.org/10.22146/jfs.45859

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