Bioformation of N-Acetylglucosamine from Shrimp Shell Chitin by Serratia marcescens PT-6 Cultured in Various pH and Temperature

https://doi.org/10.22146/jfs.25961

Bekti Wulan Sari(1*), Nurul Binti Isnaini(2), Indun Dewi Puspita(3), Amir Husni(4), Ustadi Ustadi(5)

(1) Department of Fisheries, Faculty of Agriculture, UGM.
(2) Department of Fisheries, Faculty of Agriculture, UGM.
(3) Department of Fisheries, Faculty of Agriculture, UGM.
(4) Department of Fisheries, Faculty of Agriculture, UGM.
(5) Department of Fisheries, Faculty of Agriculture, UGM.
(*) Corresponding Author

Abstract


The study aimed to determine the effect of pH and temperature on N-Acetylglucosamine (GlcNAc) formation from shrimp shell chitin by Serratia marcescens PT-6 fermented in chitin broth medium. Parameters examined on GlcNAc formation include bacteria growth, chitinase activity (U/ml), and GlcNAc concentration in medium (mg/ ml). Bacteria growth was measured by turbidity (OD600), while chitinase activity and GlcNAc concentration in the medium were analyzed quantitatively by colorimetric assay. The variation of initial pH examined in chitin broth medium was 5; 6; 7; 8, while temperature variation was 30°C; 37°C; dan 40°C. The results show that maximum concentration of GlcNAc formed by Serratia marcescens PT-6 was 39.30 mg/ml on day 5 of fermentation, at pH 7 and temperature of 30°C. Chitinase activity on the same day was 0.007 U/ml, while OD600 value of the culture was 0.51 indicating the bacteria were in the log phase. This research implies that Serratia marcescens PT-6 is potential to be optimized further for the bioconversion process of GlcNAc from shrimp shell waste through an enzymatic method by modifying its growth environment.

Keywords


Chitinase; chitin; N-Acetylglucosamine; Serratia marcescens PT-6

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References

Arnold, L.D. & Solomon. 1986. Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington.

Azhar, M., J. Effendi, E. Syofyeni, R.M. Lesi, & S. Novalina. 2010. Pengaruh Konsentrasi NaOH dan KOH Terhadap Derajat Deasetilasi Kitin dari Limbah Kulit Udang. Jurnal Eksakta. 1: 1-8.

Brurberg, M.B., I.F. Nes, & V.G.H. Eijsink. 1996. Comparative studies of chitinases A and B from Serratia marcescens. Microbiology. 142: 15811589.

Brzezinska, M.S. & W. Donderski. 2001. Occurrence and Activity of the Chitinolytic Bacteria of Aeromonas Genus. Polish Journal of Environmental Studies. 10: 2731.

Chakrabortty, S., S. Bhattacharya, & A. Das. 2012. Optimization of Process Parameters for Chitinase Production by a Marine Isolate of Serratia marcescens. International Journal of Pharmacy and Biological Sciences. 2: 22307605.

Chen, J.K., C.R. Shen, & C.L. Liu. 2010. N-Acetylglucosamin: Production and Applications. Marine Drugs. 8: 2493-2516.

Donderski, W. & M. Trzebiatowska. 1999. Chitinase Activity Production by Planktonic, Benthic and Epiphytic Bacteria Inhabiting the Moty Bay of The Jeziorak Lake (Poland). Polish Journal of Environmental Studies. 8: 215–220.

Han, K.I., B.B. Patnaik, A.R. Cho, H.K. Lim, J.M. Lee, Y.G. Jang, Y.S. Jeong, T.K. Yoo, G.S. Lee, & M.D. Han. 2014. Characterization of Chitinase Producing Serratia and Bacillus Strains Isolated from Insects. Entomological Research. 44: 109120.

Hardjito, L., A. Huq, & R.R. Colwell. 2002. The Influence of Environmental Conditions on the Production of Pigment by Serratia marcescens. Biotechnology and Bioprocess Engineering. 7: 100-104.

Hargono, A. & I. Suamntri. 2008. Pembuatan Kitosan dari Limbah Cangkang Udang Serta Aplikasinya dalam Mereduksi Kolesterol Lemak Kambing. Reaktor. 12: 53-57.

Hsu, S. & C. Lockwood. 1974. Powdered Chitin Agar as a Selective Medium for Enumeration of Actynomycetes in Water and Soil. Applied Microbiology. 29: 422-426.

Kholifah, A. 2015. Isolasi & Identifikasi Bakteri-Bakteri Kitinolitik dari Sedimen Tambak Udang. Fakultas Pertanian. Universitas Gadjah Mada. Skripsi.

Khusniati, T., N. Widhyastuti, I. Saskiawan, A. Choliq, & R. Handayani. 2012. Peningkatan Kualitas Produk Susu dengan N-Asetilglukosamina dan β-Galaktosidase di Jawa. Tim Pelaksana Insentif Peningkatan Kemampuan Peneliti dan Perekayasa. Lembaga Ilmu Pengetahuan Indonesia.

Kuddus, S.M. & R.I.Z. Ahmad. 2013. Isolation of Novel Chitinolytic Bacteria and Production Optimization of Extracellular Chitinase. Journal of Genetic Engineering and Biotechnology. 11: 39-46.

Lamine, B.M., B.M. Lamine, & A. Bouziane. 2012. Optimization of the Chitinase Production by Serratia marcescens DSM 30121T and Biological Control of Locusts. Journal Biotechnol Biomaterial. 2: 133-137.

Maggadani, B.P. 2012. Optimasi Produksi N-Asetilglukosamin dari Kitin Menggunakan Kitinase Hasil Isolasi Bakteri. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Indonesia. Thesis.

Minami, S. & Y. Okamoto. 2007. Drug For Remedy Or Treatment of Wound. European Patent Specification. Bulletin. 2014/17.

Muharni. 2009. Isolasi dan Identifikasi Bakteri Penghasil Kitinase dari Sumber Air Panas Danau Ranau Sumatera Selatan. Jurnal Penelitian Sains. 9: 12-15.

Reissig, J.L., J.L. Strominger, & L.F. Leloir. 1955. A Modified Colorimetric Method for the Estimation of N-Acetylamino Sugars. The Journal of Biological Chemistry. 217: 959-966.

Sayo, T., S. Sakai, & S. Inoue. 2004. Synergistic Effect of N-asetylglucosamine and Retinoids in Hyaluronan Production in Human Keratinocytes. Skin Pharmacology and Physiology. 17: 77-83.

Shonnard, D.R. 2015. Chapter 6: How Cells Grow. Department of Chemichal Engineering. Michigan Technological University.

Sudhakar & P. Nagarajan. 2011. Production of Chitinase by Solid State Fermentation from Serratia marcescens. International Journal of ChemTech Research. 3: 590-598.

Sumantha, A., C. Larroche, & A. Pandey. 2006. Microbiology and Industrial of Food Grade Protease: A Perspective. Food Technology & Biotechnology. 44: 211-220.

Suresh, P.V. & M. Chandrasekaran. 1999. Impact of Process Paramaters on Chitinase Production by an Alkalophilic Marine Beauveria bassiana in Solid State Fermentation. Process Biochemistry. 34: 257-267.

Swastawati, F., I. Wijayanti, & E. Susanto. 2008. Pemanfaatan Limbah Kulit Udang Menjadi Edible Coating untuk Mengurangi Pencemaran Lingkungan. Jurnal. 4: 101-106.

Triwijayani, A. U. 2016. Identifikasi Bakteri Kitinolitik dari Sedimen Tambak Udang dan Karakteristik Kitinasenya. Fakultas Pertanian. Universitas Gadjah Mada. Skripsi.

Wang, S. L., B.S. Lin, T.W. Liang, C.L. Wan, P.C. Wu, & J.R. Liu. 2010. Purification and Characterization of Chitinase from New Species Strain, Pseudomonas sp. TKU008. Journal of Microbiology and Biotechnology. 20: 10011005.

Widhyastuti, N. 2010. Purifikasi N-Asetil-Dglukosamina Hasil Sintesa Secara Enzimatis untuk Bahan Obat dan Pangan Fungsional. Pusat Penelitian Biologi Lembaga Ilmu Pengetahuan Indonesia. Laporan Akhir Program Insentif Peneliti dan Perekayasa LIPI.

Yurnaliza. 2002. Senyawa Khitin dan Kajian Aktivitas Enzim Mikrobial Pendegradasinya. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sumatra Utara. Kajian Pustaka.



DOI: https://doi.org/10.22146/jfs.25961

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