Development and validation of atomic absorption spectrometry for the determination of zink and mercury analyzer for determination of Mercury in cream cosmetics

Abdul Rohman(1*), Erni Wijayanti(2)

(2) 2 Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia
(*) Corresponding Author


An atomic absorption spectrometry for the quantification of zink (Zn) and mercury analyzer  for the quantification of mercury (Hg) levels in cream cosmetic were developed and validated. The method was validated for linearity and range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The standard curves were linear over the concentration of 0.1-1.2 µg/mL (Zn) and 0.05-2.0 µg/L (Hg) with coefficient of correlation (r) > 0.99. The detection limits obtained were 2.8614 µg/g sample (Zn) and 0.4749 ng/g sample. The quantification limit  obtained were 9.5281 µg/g sample (Zn) and 1.5827 ng/g sample. The relative standar deviation (RSD) values found to be 8.67 % for its intraday precision and 9.89 % for its interday precision (Zn) and 12.69 % for its intraday precision and 7.17 % for its interday precision (Hg). These RSD values are lower than those required by RSD Horwitz unction. The mean recovery percentage was 94.28 % (for Zn) and 78.65% (for Hg). These developed methods were succesfully used for determination of Zn and Hg in cream cosmetics products.


Key words: mercury, zink, atomic absorption spectroscopy, mercury analyzer, cream cosmetic

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Eurachem, 1998. The fitness for purpose of analytical method: A laboratory guide to method validation and related topics, accessed from:,18/04/2011.

Giunta, F., D. Dilandro, and M. Chiarmda, 1983. Severe acute poisoning from the Ingestion of a permanent Wave solution of mercuric chloride. Human Toxicol., 2: 243-246.

Gonzalez, A.G. and M.A. Herrador, 2007. A Practical guide to analytical method validation, including measurement uncertainty and accuracy profiles. Trends Anal. Chem., 26: 227-238.

International Conference on Harmonisation (ICH), 1994. Validation of Analytical Procedures: Text and Methodology, accessedfrom:, 19/04/2011.

ISO/IEC 17025. 2005. General requirements for the competence of testing and calibration laboratories.

Khopkar, S.M., 1990. Basic Concept of Analytical Chemistry, UI Press, Jakarta.

Manahan, E., 1994. Environmental Chemistry, Lewis publishers, Dallas.

Noviana, E., D.P. Heri, Astuti, and A. Rohman, 2012. Validation of mercury analyzer for determination of mercury in snake fruit. Int. Food Res. J., 19 (3): 933-936.

Plum, L.M., L. Rink, and H. Haase, 2010. The Essential Toxin: Impact of Zinc in Human Health. J. Environ. Res., 7: 1342-1365.

Taverniers, I., M. De Loose, and E. Van Bockstaele, 2004. Trends in quality in the analytical laboratory: Analytical method validation and quality assurance. Trends Anal. Chem., 23: 535 – 552.

Vogel A.I., 2001, A text book of quantitative inorganic chemical analysis including elementary instrumental analysis, 5th edition pp 80, Logman Inc., London.


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Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.