Piptadenia Africana: Enhances Weight Gain, Oxidative Stress, Hyperlipidemia in Normal and Hypercholesterolemic Male Wistar Rat


Nwozo Sarah Onyenibe(1*), Oyinloye Babatunji Emmanuel(2)

(1) Nutrition and Industrial Biochemistry Unit, Biochemistry Department, FBMS, College of Medicine, University of Ibadan, Ibadan, Nigeria
(2) Nutrition and Industrial Biochemistry Unit, Biochemistry Department, FBMS, College of Medicine, University of Ibadan, Ibadan, Nigeria Department of Biochemsitry, College of Science, Afe Babalola University, Ado Ekiti, Nigeria
(*) Corresponding Author


Poyphenol rich stem bark of Piptadenia africana (PA) is used to treat gastric pain, fever, cough and we evaluated the effect of daily, continual intake of PA for eight weeks in hypercholesterolemic rats. Thirty six rats were divided into six equal groups, A (positive-control); B (cholesterol) (negative-control); C (100 mg/kg bwt PA); D (200 mg/kg bwt PA); E (cholesterol+100 mg/kg bwt PA) and F (cholesterol+200 mg/kg bwt PA). Corn oil served as vehicle for both cholesterol (40 mg/kg/0.3ml) and PA. PA caused increased weight-gain, no enlarged organs but decreased their protein concentrations relative to control. Serum triglyceride, total cholesterol and LDL-cholesterol were elevated in PA only and PA co-treated cholesterol rats relative to both control groups, culminating in high atherogenic index. Lipidperoxidation increased dose dependently while glutathione-peroxidase, glutathione-S-transferase, reduced glutathione, superoxide dismutase and catalase decreased in PA treated groups relative to controls. Histological examination revealed necrosis of hepatocytes in groups D and moderate coagulation of necrosis of tubules of renal medulla in C. Continuous usage of PA may not be totally safe as it enhances weight gain, unhealthy lipid profile, increase chances of cardiovascular disease, elicits oxidative stress and induces organ toxicity.


Key words: atherogenic-index, lipidperoxidation, lipid profile, organ toxicity, Piptadenia africana.

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Brautbar N, Williams II J. Industrial solvents and solvent and liver toxicity: risk assessment, risk factor and mechanism: review. International Journal of Hygiene and Environmental Health (IJHEH). 2002; 205: 479-491.

Clarke M. “Saponin for health.” Nutritkion J. 2000; 2: 16

Iwu MM. Handbook of African Medicinal plants. CRC press, Boca Raton, Florida. 1993.

Leonardo DCL, Franco A, Gustavo ATL, Luciano MA, Luis FMES, Gabriel PDS, et al.Toxicological evaluation by in-vitro and in-vivo assays of an aqueous extract prepared from Echnindorus macrophyllus leaves Toxicol Lett. 2000; 116:189-196.

Letouzey R. Manuel d Botanique Forestière de l’ Afrique Tropicale, II A Imprimerie Jouve, Paris 6e, France; 1969.

Leudeu BCT, Tchiengang C, Barbe F, Nicolas B, Gueani JL. Ricindendron heutelotti (Bail) or Tetracarpidium conophorum (Mull) oils fed to rats lower blood lipids. Nutri. Res. 2009; 29(7): 503-509.

Lüscher TF, Landmesser U, Eckardstein AV, Fogelman AM. High-density lipoprotein vascular protective effects, dysfunction, and potential as therapeutic target. Circ Res. 2014; 114: 171-182.

Mallika V, Goswami B, Rajappa M. Atherosclerosis pathophysiology and the role of novel risk factors, a clinical biochemistry perspective. Angiology. 2007; 58: 513 - 522.

Malomo SO. Toxicological Implication of Ceftriaxone Administration in Rats. Nig. J. Biochem. & Mol. Biol. 2000; 15(1): 33-38.

Mbouuangouere RN, Tane P, Choudhary MI, Djemgou P, Ngadjui BT, Ngamga D. Piptadenol A-C and α-glucosidase inhibitor from Piptadenia africana Res J. Phytochemistry 2008; 2(1): 27-34.

Mbouuangouere RN, Tane P, Ngamga D, Djemgou P, Choudhary MI, Ngadjui BT. Piptaderol from Piptadenia africana. Afr J Trad CAM. 2007; 4(3): 294-298.

Nwozo SO, Orojobi F, Adaramoye OA. Hypolipidemic and antioxidant potentials of Xylopia aethiopica seed extract in hypercholesterolaemic rats. J. Med. Food. 2011; 14 (1/2): 114-119.

Oyedemi SO, Bradley G, Afolayan AJ. Toxicological effect of aqueous stem bark extract of Strychnos henigsii Gilg in Wistar rats. J. Nat Pharm. 2009; 1:33-39

Sharma R. “Nutraceuticals and nutraceutical supplementation criteria in cancer: literature survey.” Open Nutraceuticals J. 2009; 2(2): 92-106.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. free radicals, metals and antioxidants in oxidative stress induced cancer. Chemico-Biological Interactions. 2006; 160: 1-40.

Yakubu MT, Bilbis LS, Lawal M, Akanji MA. Effect of Repeated administration of sildenafl citrate on selected enzyme activities of liver and kidney of male albino rats. Nig. J. Pure & Appl. Sci. 2003; 18: 1395-1400.

DOI: https://doi.org/10.14499/jfps

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