Analytical Method Validation of Selected Organochlorine Pesticides in Fortified Rice Using Gas Chromatography with Electron Capture Detector

https://doi.org/10.14499/jfps

Mutia Kusuma Wardani(1), Bambang Sutriyanto(2), Endang Lukitaningsih(3), Abdul Rohman(4*)

(1) Faculty of Pharmacy, Gadjah Mada University
(2) 
(3) 
(4) 
(*) Corresponding Author

Abstract


Gas chromatography with electron capture detector (GC-ECD) has been validated for simultaneous determination of selected organochlorine pesticides namely heptachlor, endosulfan, dieldrin, dan p,p’-dichlorodiphenyltrichloroethane (p,p’-DDT) using aldrin as standard internal. Some parameters for analytical method validation were evaluated, i.e. linearity, precision, sensitivity expressed with limit of detection and limit of quantification, and recovery. GC-ECD was linear for determination of these pesticides with r values ≥ 0.99. The values of LOD obtained were 2.50, 2.97, 1.61, and 2.54 ng/mL for heptachlor, endosulphan, dieldrin, and p,p’-DDT, respectively. Furthermore, the relative standard deviation (RSD) values obtained for the assessment of analytical precision were lower than those required by Horwitz. Finally, the used GC-ECD method was accurate for determination of these organochlorine psticides in fortified rice samples which were indicated by the acceptable recovery percentages.


Full Text:

PDF



DOI: https://doi.org/10.14499/jfps

Article Metrics

Abstract views : 274 | views : 459

Refbacks

  • There are currently no refbacks.


Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.