Untranslated region-5' and viral protein 1-based genetic stability analysis of bulk polio in Indonesia 2010-2019

https://doi.org/10.19106/JMedSci005304202106

Andi Yasmon(1), Normasari Normasari(2*), Fithriyah Fithriyah(3), Fadilah Fadilah(4)

(1) Microbiology Department, Faculty of Medicine, Universitas Indonesia, Jakarta
(2) Master Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta/National Quality Control Laboratory of Drug and Food, National Agency of Drug and Food Control
(3) Microbiology Department, Faculty of Medicine, Universitas Indonesia, Jakarta
(4) Chemistry Department, Faculty of Medicine, Universitas Indonesia, Jakarta
(*) Corresponding Author

Abstract


Cases of vaccine-associated paralytic poliomyelitis (VAPP) continued increasingly from 2010-2019 in the world. Oral polio vaccine (OPV) is the live attenuated virus-based vaccine that could genetically revert to neurovirulent during the vaccine production process or when the virus replicates in the human body. The poliovirus neurovirulence is determined by the UTR-5' region and VP1 coding region. UTR-5' played a role in protein translation and VP1 was responsible for the immunogenicity of the virus. Some reported mutations in UTR-5' and VP1 could affect the neurovirulence of poliovirus. In this study, we analyzed the genetic stability of the UTR-5' and VP1 in the bulk of OPV types -1 and -3 produced in 2010 - 2019. The results of the analysis of UTR-5' sequences in Sabin strain types-1 and -3 and VP1 sequences on Sabin virus type 1 did not show any mutations. Meanwhile, the VP1 sequences in Sabin strain type 3 showed nucleotide mutation C2493U that caused the substitution amino acid Thr6Ile amino acid in all samples of the type 3 bulk polio test. Based on the results of in silico analysis, this mutation in VP1 did not contribute significantly to the neurovirulence of the virus.


Keywords


poliovirus; UTR-5’; VP1; Sabin; genetic characterization

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DOI: https://doi.org/10.19106/JMedSci005304202106

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