The effect of cyperus rotundus root ethanol extract on the epithelization in the healing process on skin excision wound

Abstract

Ch. Tri Nuryana, E. Suryadi & Harijadi - The Influence of Ethanol Extract of Purple Nutsedge (Cyperus
rotundus) on Epithelization of Skin Wound Healing Process
Background: If skin is wounded, epithelial cells will migrate to the wound area in order to keep homeostatic.
Epithelization consists of mitotic and proliferation of epithelial cells. Rhizome of purple nutsedge (Cyperus
rotundus) contains cyperene, flavonoid, ²-sitosterol dan ascorbic acid that can accelerate epithelization in
wound healing process and has been used in traditional medicine.
Objective: To investigate the influence of ethanol extract of Purple Nutsedge (Cyperus rotundus) in
epithelization of wound healing process.
Methods: Fourty five Balb/c male mice 8-10 weeks old, 25-35 g weight, were excised punch biopsy on
back 0.5 cm right and left of columna vertebralis, 2.5 cm in cutis from the ear area. The mice were
divided into 5 groups. Negative control group was treated with vehicle of extract, positive control group
was treated by gel containing placenta extract 1% & neomycin sulfate 0.5% and the others were treated
with C. rotundus 1%, 2%, and 4% in concentration. Each of the groups was divided into 3 subgroups.
Each of the subgroups composed of 3 mice based on the period of termination, i.e.: 3rd, 7th and 12th day
after wounded. Histological evaluation was done to investigate the thickness of epithelial layer.
Results: The data were analyzed by Two-way Anova and the results showed that there was a significant
difference (p<0.05) in every decapitation period, groups, and interaction between decapitation period and
groups. The results of LSD test showed that there was a significant difference (p<0.05) in the thickness
of epithelial layer between negative control and C. rotundus groups.
Conclusion: Cyperus rotundus extract accelerates epithelization process of wound healing in mice.
Key words: Wound healing - Cyperus rotundus - epithelization - mice