Activity of -terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines



Puguh Indrasetiawan Indwiani Astuti Mustofa(1*)

(1) 
(*) Corresponding Author

Abstract


Previous study has shown that -terpineol has a cytotoxic effect on NCI-H69 cancer cell line.
This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of -
terpineol in T47D cell lines. The cytotoxic activity of -terpineol was determined using MTT cell
viability assay, while apoptosis was tested by the acridine orange-ethidium bromide staining
method. The cell cycle was evaluated by flowcytometry method. The results showed that -
terpineol had cytotoxic effect on the T47D cells with the IC50 value of 135.00 ± 8.74 M.
Furthermore, -terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometry
analysis showed that -terpineol induced the T47D cell distribution in Go/G1 phase, however
inhibited cell distribution in S phase. This study suggests that -terpineol has a potential anticancer
activity.





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Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Based on a work at http://jurnal.ugm.ac.id/bik/.