Comparative Study Production of Exopolysaccharide (EPS) by Lactic Acid Bacteria (L. casei and L. plantarum) in Different Media (Dates and Mulberry juice)

Elok Zubaidah(1*), Yusry Muqit Suryawira(2), Ella Saparianti(3)

(1) Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Indonesia
(2) Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Indonesia
(3) Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Indonesia
(*) Corresponding Author


Exopolysaccharides (EPS) are polysaccharides that are secreted by some strains of bacteria. E PS contribute in health improvement where it has prebiotic properties, immunostimulatory, anti- tumoral, and hypocholesterolemic effects. A number of Lactic Acid Bacteria (LAB) has ability t o synthesise Exopolysaccharides (EPS). This study aimed to determine the ability LAB (L. casei and L. plantarum B2) to produce EPS in different media (dates and mulberry juice). L. casei sh owed higher EPS production (3413.33 mg/L) than L. plantarum (3316.67 mg / L) were grown in medium dates juices. Based on the type of media, EPS production in dates juice medium higher t han mulberry juice as medium, and there are differences production of EPS by both types of isol ates (L. casei and L. plantarum) in both media. The growth rate of LAB does not always show a positive correlation with EPS formation.


Dates juice; Exopolysaccharide; LAB; Mulberry juice

Full Text:



Cerning J, Bouillanne C, Landon M, Desmazeaud M. 1992. Isolation and characterization of exopolysaccharides from slime - forming mesophilic lactic acid bacteria. J Dairy Sci, 75:692–9.

De Vuyst L, De Vin F, Kamerling JP. 2007. Exopolysaccharides from lactic acid bacteria. In:Comprehensive Glycoscience,Vol. 2.(pp. 477–518).(Editor: KamerlingJP). Oxford: Elsevier

Fox, P.F., T.P. Guinee, T. M. Cogan, P. L. H. McSweeny. 2000. Fundamental of Cheese Science. An aspen Publicat .Maryland

Hallemeersch I, De Baet S, Vandamme EJ. In:Steinbüchel A, editor. Exopolysaccharides of lactic acid bacteria. Biopolymers.Wiley VCH:Weinheim; 2002. p. 407–29.

Harrah, T., B. Panilaitis and D. Kaplan.2006. Microbial Exopolysaccharides. (

Lam J.H. and Lee Y.K. 2006. The Influence of Glucose Concentration on the Production of Exopolysaccharides (EPS) by Lactobacillus casei Shirota and the Effect of EPS on the Adherence of Escherichia coli O157:H7 to Human Intestinal Cells. Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore

Pham P. L, Dupont I., Roy D., Lapointe G, and Cerning J. 2000. Production of Exopolisaccaride by Lactobacillus rhamnosus R and Analysis of its Enzymatic De gradation during Prolonged fermentation. Appl Environ Microbiol. American Society for Microbiology. Kanada

Tallon, R., P. Bressollier and M.C. Urdaci. 2006. Isolation and Characterization of Two Exopolysaccharides Produced by Lactobacillus plantarum EP56. (

Velasco et al., 2006. Environmental factors influencing growth of and exopolysaccharide formation by Pediococcus parvulus 2.6. International Journal of Food Microbiology 111 (2006) 252–258


Article Metrics

Abstract views : 1190 | views : 2518


  • There are currently no refbacks.

Copyright (c) 2017 Elok Zubaidah, Yusry Muqit Suryawira, Ella Saparianti

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.