Antioxidant Activity of Hydroalcoholic Extract of Puguntano Herbs and Andaliman Fruits by Cuprac Methods

https://doi.org/10.22146/mot.90337

Denny Satria(1*), Aminah Dalimunthe(2), Panal Sitorus(3), Syukur Berkat Waruwu(4), Mumtaz Vadhila(5), Muhammad Wahyu(6)

(1) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara\
(2) Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara
(3) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara
(4) Faculty of Pharmacy and Health Sciences, Universitas Sari Mutiara Indonesia, Medan, Sumatera Utara
(5) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara
(6) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Sumatera Utara
(*) Corresponding Author

Abstract


Free radical damage to the body can be prevented and repaired using antioxidants. Various natural ingredients native to Indonesia contain antioxidants, which are needed to improve people's health at an affordable cost. Puguntano (Picria fel-terrae) and Andaliman (Zanthoxylum acanthopodium DC.) are natural ingredients that can be used as antioxidants. This research determined the antioxidant activity and differences in IC50 values of hydroalcoholic extracts from Puguntano (Picria fel-terrae Lour) herbs and Andaliman (Zanthoxylum acanthopodium Fruits). Hydroalcoholic extracts were prepared by maceration using 90%, 80%, 70%, 60% and 50% ethanol. Antioxidant capacity was measured using the CUPRAC method, and quercetin was used as a positive control. The results were showed that the IC50 value for hydroalcoholic extracts of Puguntano herbs (27.02 ± 0.15 µg/mL, 25.94 ± 0.22 µg/mL, 25.12 ±0.15 µg/mL, 22.07 ± 0.23 µg/mL, and 23.86 ±0.18 µg/mL) and for Andaliman fruits (41.65 ± 0.30 µg/mL, 36.59 ± 0.40 µg/mL, 34.13 ± 0.44 µg/mL, 25.46 ± 0.30 µg/mL, 25.39 ± 0.30 µg/mL) respectively and quercetin 2.21 ± 0.02 µg/mL. Hydroalcoholic extracts of Puguntano herbs and Andaliman fruits have antioxidant activity, with each concentration having an IC50 of less than 50 µg/mL. The test sample's antioxidant activity is indicated by its lower IC50 value.


Keywords


Antioxidant; Hydroalcoholic extract; IC50; Picria fel-terrae Lour; Zanthoxylum acanthopodium DC Fruits

Full Text:

PDF


References

Angela, I. F. D., Dalimunthe, A., Harahap, U., & Satria, D. (2023). Effect of andaliman (Zanthoxylum acanthopodium DC.) ethanol extract on doxorubicin-induced toxicity on hematology in male rats. Journal of Drug Delivery and Therapeutics, 13(3), 27–29. https://doi.org/10.22270/jddt.v13i3.5975

Anjelisa, Z.H.P., Haro, G., & Satria, D. (2019). Cytotoxicity Activity of Ethanol Extract of Andaliman Fruits (Zanthoxylum acanthopodium DC.) towards 4T1 Breast Cancer Cells. Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR), 02(2), 31–35.

Auliafendri, N., Rosidah, R., Yuandani, Y., Suryani, S., & Satria, D. (2019a). The inhibitory activity of picria fel-terrae lour herbs extract on nitric oxide production toward RAW 264.7 cells induced by lipopolysaccharide. Open Access Macedonian Journal of Medical Sciences, 7(22), 3737–3740. https://doi.org/10.3889/oamjms.2019.493

Auliafendri, N., Rosidah, Yuandani, Suryani, S., & Satria, D. (2019b). The immunomodulatory activities of Picria Fel-Terrae lour herbs towards RAW 264.7 cells. Open Access Macedonian Journal of Medical Sciences, 7(1), 24–28. https://doi.org/10.3889/oamjms.2019.017

Batiha, E.G., Beshbishy, A. M., Ikram, M., Mulla, Z. S., Abd El-Hack, M. E., Taha, A. E., Algammal, A. M., & Ali Elewa, Y. H. (2020). The pharmacological activity, biochemical properties, and pharmacokinetics of the major natural polyphenolic flavonoid:

Quercetin. In Foods (Vol. 9, Issue 3). MDPI Multidisciplinary Digital Publishing Institute. https://doi.org/10.3390/foods9030374

Bratovcic, A. (2020). Antioxidant Enzymes and their Role in Preventing Cell Damage.

Capanoglu, E., Kamiloglu, S., Demirci Cekic, S., Sozgen Baskan, K., Avan, A. N., Uzunboy, S., &

Apak, R. (2021). Antioxidant Activity and Capacity Measurement (pp. 1–66). https://doi.org/10.1007/978-3-030-45299-5_22-1

Csicsor, A., Tombácz, E., & Kulcsár, P. (2023). Antioxidant potential of humic substances measured by Folin-Ciocalteu, CUPRAC, QUENCHER-CUPRAC and ESR methods. Journal of Molecular Liquids, 391, 123294. https://doi.org/10.1016/j.molliq.2023.123294

Dalimunthe, A., Pertiwi, D., Muhammad, M., Asfianti, V., & Satria, D. (2023). The effect of ethanol percentage as solvent towards antioxidant activity of Picria fel-terrae Lour. herbs. AIP Conference Proceedings, 2626(1), 030005. https://doi.org/10.1063/5.0136102

Dalimunthe, A., Satria, D., Sitorus, P., Harahap, U., Angela, I. F. D., & Waruwu, S. B. (2024). Cardioprotective effect of hydroalcohol extract of Andaliman (Zanthoxylum Acanthopodium DC.) Fruits on Doxorubicin-Induced Rats. Pharmaceuticals, 17(3), 359. https://10.3390/ph17030359.

Di Meo, S., & Venditti, P. (2020). Evolution of the Knowledge of Free Radicals and Other Oxidants. In Oxidative Medicine and Cellular Longevity (Vol. 2020). Hindawi Limited. https://doi.org/10.1155/2020/9829176

Ezez, D., & Tefera, M. (2021). Effects of Solvents on Total Phenolic Content and Antioxidant Activity of Ginger Extracts. Journal of Chemistry, 2021, 1–5. https://doi.org/10.1155/2021/6635199

Flieger, J., Flieger, W., Baj, J., & Maciejewski, R. (2021). Antioxidants: Classification, natural sources, activity/capacity measurements, and usefulness for the synthesis of nanoparticles. In Materials (Vol. 14, Issue 15). MDPI AG. https://doi.org/10.3390/ma14154135

Gulcin, İ. (2020). Antioxidants and antioxidant methods: an updated overview. Archives of Toxicology, 94(3), 651–715. https://doi.org/10.1007/s00204-020-02689-3

Harfina, F., Bahri, S., & Saragih, A. (2012). Pengaruh Serbuk Daun Puguntano (Curanga fel-terrae Merr.) Pada Pasien Diabetes Mellitus the Effect of Puguntano Leaf Powder (Curanga fel-terrae Merr.) on Patients with Diabetes Mellitus. In Journal of Pharmaceutics and Pharmacology (Vol. 1, Issue 2).

Ionita, P. (2021). The chemistry of dpph∙ free radical and congeners. In International Journal of Molecular Sciences (Vol. 22, Issue 4, pp. 1–15). MDPI AG. https://doi.org/10.3390/ijms22041545

Ivanova, A., Gerasimova, E., & Gazizullina, E. (2020). Study of Antioxidant Properties of Agents from the Perspective of Their Action Mechanisms. Molecules, 25(18), 4251. https://doi.org/10.3390/molecules25184251

Khaerunnisa, N., Saraswati, I., Sasikirana, W., & Kunci, K. (2022). Kandungan Total Fenolik Ekstrak Metanol Buah Leunca (Solanum nigrum L.) dan Fraksi-Fraksinya. Generics: Journal of Research in Pharmacy, 2(2), 86 - 92. https://doi.org/10.14710/genres.v2i2.15716

Khiya, Z., Oualcadi, Y., Gamar, A., Berrekhis, F., Zair, T., & Hilali, F. EL. (2021). Correlation of Total Polyphenolic Content with Antioxidant Activity of Hydromethanolic Extract and Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco. Journal of Chemistry, 2021, 1–11. https://doi.org/10.1155/2021/8585313

Kumalasari, E., Septia, A., Rizki Febrianti, D., & Aisyah N. (2023). Penetapan Kadar Flavonoid Total Ekstrak Etanol dan Fraksi Etanol, Fraksi Kloroform, Fraksi N-Heksana, Fraksi Air, Fraksi Etil Asetat dari Daun Bawang Dayak (Eleutherine palmifolia (L.) Merr.). In Merr.). 2023 (Vol. 9, Issue 2). https://doi.org/10.51352/jim.v9i2.678

Lubis, M. R., Haryani, R., Safriana, S., & Satria, D. (2019). Ethanolic extract of herb pugun tanoh (Picria fel-terrae lour.) modulates TCD4+ and TCD8+ cell profile of doxorubicin-induced immuno-suppressed rats. Open Access Macedonian Journal of Medical Sciences, 7(22), 3774–3776. https://doi.org/10.3889/oamjms.2019.501

Muhammad M., Putra E.D., Cintya H., & Satria, D. (2023). The Effect of Solvent Towards Antioxidant Activity of Vernonia amygdalina Delile Leaves. RASAYAN Journal of Chemistry, 16(02), 760–765. https://doi.org/10.31788/rjc.2023.1628059

Martemucci, G., Costagliola, C., Mariano, M., D’andrea, L., Napolitano, P., & D’Alessandro, A. G. (2022). Free Radical Properties, Source and Targets, Antioxidant Consumption and Health. Oxygen, 2(2), 48–78. https://doi.org/10.3390/oxygen2020006

Munteanu, I. G., & Apetrei, C. (2021). Analytical

methods used in determining antioxidant activity: A review. In International Journal of Molecular Sciences (Vol. 22, Issue 7). MDPI AG. https://doi.org/10.3390/ijms22073380

Nazliniwaty, Hanafiah, O. A., Pertiwi, D., Satria, D., & Muhammad, M. (2021). Antioxidant activity, total phenolic and total flavonoid content of hydroalcoholic extract of Artocarpus lacucha Buch-Ham. Leaves. AIP Conference Proceedings, 2342(1), 080010. https://doi.org/10.1063/5.0045440

Ompusunggu, N. P., & Irawati, W. (2021). Andaliman (Zanthoxylum Acanthopodium DC.), a Rare Endemic Plant from North Sumatra that Rich in Essential Oils and Potentially as Antioxidant and Antibacterial. Jurnal Biologi Tropis, 21(3), 1063–1072. https://doi.org/10.29303/jbt.v21i3.2961

Özyürek, M., Güçlü, K., & Apak, R. (2011). The main and modified CUPRAC methods of antioxidant measurement. In TrAC - Trends in Analytical Chemistry (Vol. 30, Issue 4, pp. 652–664). https://doi.org/10.1016/j.trac.2010.11.016

Pande, J., & Chanda, S. (2020). Mini Review: Screening of Antioxidant Properties of Some Medicinal Plants. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3576589

Parcheta, M., Świsłocka, R., Orzechowska, S., Akimowicz, M., Choińska, R., & Lewandowski, W. (2021). Recent Developments in Effective Antioxidants: The Structure and Antioxidant Properties. Materials, 14(8), 1984. https://doi.org/10.3390/ma14081984

Pisoschi, A. M., Pop, A., Iordache, F., Stanca, L., Predoi, G., & Serban, A. I. (2021). Oxidative stress mitigation by antioxidants - An overview on their chemistry and influences on health status. In European Journal of Medicinal Chemistry (Vol. 209). Elsevier Masson s.r.l. https://doi.org/10.1016/j.ejmech.2020.112891

Saha, A., Basak, B. B., Manivel, P., & Kumar, J. (2021). Valorization of Java citronella (Cymbopogon winterianus Jowitt) distillation waste as a potential source of phenolics/antioxidant: influence of extraction solvents. Journal of Food Science and Technology, 58(1), 255–266. https://doi.org/10.1007/s13197-020-04538-8

Satria, D., Silalahi, J., Haro, G., Ilyas, S., & Hasibuan, P. A. Z. (2019a). Cytoprotective activity of ethylacetate fraction of picria fel-terrae lour. Herbs. Open Access Macedonian Journal of Medical Sciences, 7(22), 3865–3867. https://doi.org/10.3889/oamjms.2019.521

Satria, D., Silalahi, J., Haro, G., Ilyas, S., & Hasibuan, P. A. Z. (2019b). Cell cycle inhibition of ethylacetate fraction of zanthoxylum acanthopodium DC. Fruit against T47D cells. Open Access Macedonian Journal of Medical Sciences, 7(5), 726–729. https://doi.org/10.3889/oamjms.2019.178

Satria, D., Silalahi, J., Haro, G., Ilyas, S., & Hasibuan, P. A. Z. (2019c). Chemical analysis and cytotoxic activity of N-hexane fraction of Zanthoxylum acanthopodium DC. fruits. Rasayan Journal of Chemistry, 12(2), 803–808. https://doi.org/10.31788/RJC.2019.1225180

Satria, D., Silalahi, J., Haro, G., Ilyas, S., & Hasibuan, P. A. Z. (2019c). Cytoprotective activity of ethylacetate fraction of picria fel-terrae lour. Herbs. Open Access Macedonian Journal of Medical Sciences, 7(22), 3865–3867. https://doi.org/10.3889/oamjms.2019.521

Silalahi, M., Supriatna, J., Walujo, E. B., & Nisyawati. (2015). Local knowledge of medicinal plants in sub-ethnic Batak Simalungun of North Sumatra, Indonesia. Biodiversitas, 16(1), 44–54. https://doi.org/10.13057/biodiv/d160106

Spiegel, M. (2022). Current Trends in Computational Quantum Chemistry Studies on Antioxidant Radical Scavenging Activity. Journal of Chemical Information and Modeling, 62(11), 2639–2658. https://doi.org/10.1021/acs.jcim.2c00104

Swallah, M. S., Sun, H., Affoh, R., Fu, H., & Yu, H. (2020). Antioxidant Potential Overviews of Secondary Metabolites (Polyphenols) in Fruits. International Journal of Food Science, 2020, 1–8. https://doi.org/10.1155/2020/9081686

Syari, D. M., Rosidah, R., Hasibuan, P. A. Z., Haro, G., & Satria, D. (2019). Evaluation of cytotoxic activity alkaloid fractions of zanthoxylum acanthopodium DC. Fruits. Open Access Macedonian Journal of Medical Sciences, 7(22), 3745–3747. https://doi.org/10.3889/oamjms.2019.495

Vuolo, M. M., Lima, V. S., & Maróstica Junior, M. R. (2019). Phenolic Compounds. In Bioactive Compounds (pp. 33–50). Elsevier. https://doi.org/10.1016/B978-0-12-814774-0.00002-5

Xiao, F., Xu, T., Lu, B., & Liu, R. (2020). Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60–69. https://doi.org/10.1002/fft2.10

Yu, M., Gouvinhas, I., Rocha, J., & Barros, A. I. R. N. A. (2021). Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Scientific Reports, 11(1), 10041. https://doi.org/10.1038/s4159



DOI: https://doi.org/10.22146/mot.90337

Article Metrics

Abstract views : 1650 | views : 742

Refbacks



Bookmark and Share


Copyright (c) 2024 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada