Determination of Anticancer from EtOAc Fraction of Spatholobus Littoralis Hassk and of Molecular Structure Prediction

https://doi.org/10.22146/mot.88894

Dodi Iskandar(1*), Nashi Widodo(2), Warsito Warsito(3), Masruri Masruri(4), Rollando Rollando(5), Muhammad Hilmi Afthoni(6), Ichsan Ichsan(7), Adi Hermansyah(8)

(1) Polytechnic State Pontianak, Pontianak, West Kalimantan 78124
(2) Brawijaya University, Malang, East Java 65145
(3) Brawijaya University, Malang, East Java 65145
(4) Brawijaya University, Malang, East Java 65145
(5) Ma Chung University, Malang, East Java 65151
(6) University of Jember, East Java 68121
(7) Polytechnic State Pontianak, Pontianak, West Kalimantan 78124
(8) Faculty of Pharmacy, Universitas Muhammadiyah Yogyakarta, Bantul, Yogyakarta 55183
(*) Corresponding Author

Abstract


In recent years, the search for new anticancer drugs from local plants in Indonesia has become an interesting topic for research. One of the typical Indonesian local plants that have been used by the community as an anticancer traditional medicine (jamu) is Spatholobus littoralis Hassk (SLH) or better known as Bajakah. The native Dayak people of Kalimantan have used it for years for the treatment of breast cancer. In this study, the separation of single compounds from the ethyl acetate fraction of SLH stems was carried out, their molecular structure was analyzed, and anticancer bioactivity was tested. The isolation used the preparative HPLC method. The elucidation of the isolates from the ethyl acetat fraction used LCMS-MS, FTIR, and NMR. Anticancer bioactivity was determined using the MTT bioassay. The result is the acquisition of two isolates; it has been predicted that both are pseudobaptinin. The anticancer IC50 of the pseudobaptinin was 62.85 ppm, and Pseudobaptinin with impurities was 145.60 ppm.


Keywords


Anticancer; Cytotoxic Pseudobaptinin; Spatholobus Littoralis Hassk

Full Text:

PDF


References

Anand, P., Kunnumakara, A. B., & Sundaram, C. (2008). Cancer is a Preventable Disease that Requires Major Lifestyle Changes. Pharmaceutical Research, 25(9), 2097–2116. https://doi.org/10.1007/s11095-008-9661-9

Dhyani, P., Quispe, C., Sharma, E., Bahukhandi, A., Sati, P., Attri, D. C., Szopa, A., Sharifi-Rad, J., Docea, A. O., Mardare, I., Calina, D., & Cho, W. C. (2022). Anticancer potential of alkaloids: A key emphasis to colchicine, vinblastine, vincristine, vindesine, vinorelbine and vincamine. Cancer Cell International, 22(1), 206. https://doi.org/10.1186/s12935-022-02624-9

Effendi, S. A. (2021). Potensi Menjanjikan Pendapatan Perpajakan Wajib Pajak. Buletin APBN, VI(7), 3.

Elufioye, T., Abdul, A., & Moody, J. (2017). Cytotoxicity studies of the extracts, fractions, and isolated compound of Pseudocedrela kotschyi on cervical cancer (HeLa), breast cancer (MCF-7) and skeletal muscle cancer (RD) Cells. Pharmacognosy Research, 9(1), 46. https://doi.org/10.4103/0974-8490.199776

Faller, B. A., & Pandit, T. N. (2011). Safety and Efficacy of Vinorelbine in the Treatment of Non-Small Cell Lung Cancer. Clinical Medicine Insights: Oncology, 5, CMO.S5074. https://doi.org/10.4137/CMO.S5074

Fitriani, A. (2019). Fenomena Kayu Bajakah Dalam Kajian Hukum Perlindungan Konsumen Dan Hukum. Proceeding Antasari International Conference, 1(1), 20. https://jurnal.uin-antasari.ac.id/index.php/proceeding/article/view/3726

Gayatri, D., Efremov, L., Mikolajczyk, R., & Kantelhardt, E. J. (2021). Quality of Life Assessment and Pain Severity in Breast Cancer Patients Prior to Palliative Oncology Treatment in Indonesia: A Cross-Sectional Study. Patient Preference and Adherence, Volume 15, 2017–2026. https://doi.org/10.2147/PPA.S320972

Iskandar, D., Widodo, N., Warsito, Masruri, Rollando, & Antang, Y. P. P. (2022). Phenolic content, antioxidant, cytotoxic of fractions of Spatholobus littoralis Hassk from Kalimantan, Indonesia. Journal of Hunan University Natural Sciences, 49(3), 14–23. https://doi.org/10.55463/issn.1674-2974.49.3.2

Kaya Cakır, H., & Eroglu, O. (2021). In vitro anti-proliferative effect of capecitabine (Xeloda) combined with mocetinostat (MGCD0103) in 4T1 breast cancer cell line by immunoblotting. Iranian Journal of Basic Medical Sciences, 24(11). https://doi.org/10.22038/ijbms.2021.58393.12971

Khazir, J., Mir, B. A., Pilcher, L., & Riley, D. L. (2014). Role of plants in anticancer drug discovery. Phytochemistry Letters, 7, 173–181. https://doi.org/10.1016/j.phytol.2013.11.010

Kumar, S., Singh, B., & Singh, R. (2022). Catharanthus roseus (L.) G. Don: A review of its ethnobotany, phytochemistry, ethnopharmacology and toxicities. Journal of Ethnopharmacology, 284, 114647. https://doi.org/10.1016/j.jep.2021.114647

Kurek, J. (2019). Introductory Chapter: Alkaloids - Their Importance in Nature and for Human Life. In J. Kurek (Ed.), Alkaloids—Their Importance in Nature and Human Life. IntechOpen. https://doi.org/10.5772/intechopen.85400

Lee, S. G., Brownmiller, C. R., Lee, S.-O., & Kang, H. W. (2020). Anti-Inflammatory and Antioxidant Effects of Anthocyanins of Trifolium pratense (Red Clover) in Lipopolysaccharide-Stimulated RAW-267.4 Macrophages. Nutrients, 12(4), 1089. https://doi.org/10.3390/nu12041089

Smaili, F., & Boudjella, A. (2020). Epidemiology of breast cancer in women based on diagnosis data from oncologists and senologists in Algeria. Cancer Treatment and Research Communications, 25, 100220. https://doi.org/10.1016/j.ctarc.2020.100220

Sung, H., Ferlay, J., & Siegel, R. L. (2021). Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 71(3), 209–249. https://doi.org/10.3322/caac.21660

Weerapreeyakul, N., Nonpunya, A., Barusrux, S., Thitimetharoch, T., & Sripanidkulchai, B. (2012). Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chinese Medicine, 7(1), 15. https://doi.org/10.1186/1749-8546-7-15

Yanagihara, K., Ito, A., Toge, T., & Numoto, M. (1993). Antiproliferative Effects of Isoflavones on Human Cancer Cell Lines Established from the Gastrointestinal Tract. Cancer Research, 53, 8. http://cancerres.aacrjournals.org/

Yoon, J. S., Sung, S. H., Park, J. H., & Kim, Y. C. (2004). Flavonoids fromSpatholobus suberectus. Archives of Pharmacal Research, 27(6), 589–592. https://doi.org/10.1007/BF02980154



DOI: https://doi.org/10.22146/mot.88894

Article Metrics

Abstract views : 1422 | views : 827

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2024 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada