Characterization and Identification of Novel Steroids from Nauclea pobeguinii Leaves

https://doi.org/10.22146/mot.88673

Adewusi John Adepoju(1), Sunday Olusegun Oladoye(2*), Ezekiel Temidayo Ayodele(3), Adeola Victoria Falade(4), Gin Keat Lim(5), Chuan Wei OO(6)

(1) Organic Organic Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(2) Organic Organic Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(3) Organic Organic Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(4) Organic Organic Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State
(5) School of Chemical Sciences, UniversitiSains Malaysia, 11800 USM, Pulau Pinang
(6) School of Chemical Sciences, UniversitiSains Malaysia, 11800 USM, Pulau Pinang
(*) Corresponding Author

Abstract


Nauclea pobeguinii is a plant in the Rubiaceae family endemic to the swamp forest region of the world. Its extract is widely used in traditional medicine for the treatment of a wide variety of ailments such as malarial, Jaundice, gonorrhea, fever, and stomach discomfort. While other parts of the plant have been examined for the bioactive principles responsible for the medicinal properties, limited information is available in the literature as regards the leaves, hence this study. N. pobeguinii leaves were collected, air-dried, and pulverized. The pulverized sample was extracted with solvents (n-hexane, ethyl acetate, and ethanol) of varying polarity to obtain the crude extracts. Repeated column and thin layer chromatographic separation of the crude extracts afforded three compounds, which were characterized by their IR, 1H, 13C-NMR, and 2D-NMR spectral data. A comparison of the data with literature confirmed the compounds to be 2-hydroxylstigmastane acetate (1), Ergosta-5,6-epoxy-22-en-3-yl-acetate (2), and β-sitosterol (3). Compounds 1 and 2 are novel to N. pobeguinii.


Keywords


2-hydroxylstigmastane acetate; Nauclea pobeguinii; purification; Rubiaceae

Full Text:

PDF


References

Adepoju, A. J., Falade, A. V., Ayodele, E. T., Oladoye, S. O., Lim, G. K., OO, C. W., et al. (2020). Phytochemical Screening, Antibacterial and antioxidant potential of the stem bark extracts of Nauclea pobeguinii. Sci. Focus, 25, 38–45.

Adepoju, A. J., Oladoye, S, O., Ayodele, E. T., Falade, V. A., Lim, G. K and OO, C.

W.(2022). Isolation and Identification of β-sitosterol, 7-hydroxystigmast-22-en-3,6-dione and 3β, 24(S)-dihydroxycholesta-5, 25-diene-7-one fromstem bark of Nauclea pobeguinii.Traditional Medicine Journal.Vol. 27(3), p 210-217.https://DOI: 10.22146/mot.77324

Ambe, A.S., Ouattara, D., Tiebre, M.-S., Ta, B., Vroh, G.N.Z., N’Guessan, K.E. (2015). Diversité des plantes

médicinales utilisées dans le traitement traditionnel de ladiarrhée sur les marchés d’Abidjan (Côte d’Ivoire). Journal of Animal and Plant Sciences. 26, 4081 – 4096.

Bett, J. L. (2002). Medicinal plants sold in Yaoundé markets, Cameroon. African Study Monographs, 23, 47–64.

Das, K., Tiwari, R. K. S., Shrivastava, D. K. (2010). Techniques for evaluation of medicinal plant products as antimicrobial agents. Current methods and future trends. Journal of Medicinal Plant Research. 4, 104 - 111.

Hang, J., Dussault, P. (2010). A concise synthesis of β-sitosterol and other phytosterols. Steroids, 75, 879 - 88.

Haudecoeu, R., Marin, P., Basile, P., Maxime, R., Germain, S. T., Ahcene, B., et al. (2018). Traditional uses, phytochemistry and pharmacological properties of African Nauclea species. A review. J. of Ethnopharmacol, 212, 106 - 136.

Hesham, H. A., Nour, A. H., Yunus, R. M. (2016). Techniques for extraction of essential oils from plant: A review. Australian Journal of Basic and Applied Sciences. 10, 117-127.

Islam, S., & Lucky, R. (2019). A Study on different plants of the Apocynaceae family and their medicinal uses. Univers J Pharm Res, 4 (1), 42-6.

Luzakibanza, M. M. ( 2012). Etude phytochimique et pharmacologique de plantes antipaludiques utilisées en médecine traditionnelle Congolaise. Congo D. R., editor. Belgium: Université de Liège & Université de Kinshasa. Congo D. R., editor. Congo D. R., editor.

MacDonald, I., Olorunfemi, D.I. ( 2005). Plants used for medicinal purposes by the Koma people of Adamawa State, Nigeria. Indigenous Knowledge and Development Monitor, 8, 18 - 25.

Mbiantcha, M., Tsafack, E. G., Ateufack, G., & al., e. (2018). Analgesic, antiinflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiacee) in rats. Journal of Complementary and Integrative Medicine, 170, 20.

Mesia, G. K., Tona, G. L., Penge, O., & al, e. (2005). Antimalarial activities and toxicities of three plants used as traditional remedies for malaria in the Democratic Republic of Congo:Croton mubango, Nauclea pobeguiniiandPyrenacantha staudtii. Annals of Tropical Medicine & Parasitology , 99.

Njoya, E. M., Aristide, M. M., Pierra, M., Augustin, E. N., & yndy, J. M. (2017). Phytochemical analysis with free radical scavenging, nitric oxide inhibition, and antiproliferative activity of Sarcocephalus pobeguiniiextracts. BMC Complementary and Alternative Med, 17, 199 – 215.

Nur, S. A., Wan, Y.W.A, Noor, A. S. (2017). The chemical constituents from young tubers of Hydrophytum formicarum. Malaysian Journal of Analytical Sciences, 21, 291 - 297.

Ododo, M. M. Manash, K. C., Ahmed, H. D. (2016). Structure Elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora. Springer plus; 5; 1210.

Ribeiro, D. A., Damasceno, S. S., Boligon, A. A., de Menezes IRA, d. A., & da Costa, J. G. (2017). Chemical profile and antimicrobial activity of Secondatia floribunda A. DC (Apocynaceae). Asian Pac J Trop Biomed, 7 (8), 739.

Samoisy, A. K., & Mahomoodally, M. F. (2015). Ethnopharmacological analysis of medicinal plants used against noncommunicable diseases in Rodrigues Island, Indian Ocean. J Ethnopharmacol , 20-38.

Xu, B., Descalzi, G., Ye, H. R., Zhuo, M., & Wang, Y. W. (2012). Translational investigation and treatment of neuropathic pain. Journal of Molecular Pain, 8, 15.



DOI: https://doi.org/10.22146/mot.88673

Article Metrics

Abstract views : 1452 | views : 868

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2024 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada