EFFECT OF 50% ETHANOLIC EXTRACT OF PEGAGAN HERB (Centella asiatica (L.) Urban) ON CELL PROLIFERATION OF LYMPHOCYTES IN Balb/c MALE MICE INDUCED BY HEPATITIS B VACCINE

https://doi.org/10.22146/tradmedj.8861

Nikmah Nur Khusnawati(1), Suwijiyo Pramono(2), Ediati Sasmito(3*)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to evaluate the effect of 50%ethanolic extract of pegagan herb against increased lymphocyte proliferation cells in Balb/c mice that induced with hepatitis B vaccine, and also to evaluate triterpen glicosida compound (asiaticosida) of 50% ethanolic extract of pegagan herb. The test was done by 60 Balb/c male mices that divided into 6 groups : control group, testing group that was treated with pegagan extract dose of 25 mg/KgBB, 50 mg/KgBB, 100 mg/KgBB, 200 mg/KgBB and 400 mg/KgBB. All of mices were vaccinated on day 0 and days 15. Furthermore, identification of triterpen glicosida compound was done by KLT method using BAW 4:1:5 as upper eluen. Lymphocyte cells were isolated and the cells proliferation measured by MTT reduction method. Data was analyzed statistically using 95% confidence level. The result shown that 50% of ethanolic extract of pegagan herb did not give  the immuomodulatory effect while the identification test shown that 50% of extract of pegagan herb has triterpen glicosida compound (asiaticoside).


Keywords


Centella asiatica (L.) Urban, asiaticoside, immunomodulator, lymphocyte cells proliferation



References

Berridge M.V., Tan A.S., McCoy K.D., Wang A.R. 1996, The Biochemical and Cellular Basic of Cell Proliferation Assays That Use Tetrazolium Salts, Biochemica, 4 : 14-19

Jayathirtha MG., Mishra SH. 2004, Preliminary Immunomodulatory Activities of Methanol Extracts of Eclipta alba and Centella asiatica¸Phytomedicine, 11, 361-365.

Kimura Y., Sumiyoshi M., Samukawa K., Satake N., Sakanaka M. 2008, Facilitating Action of Asiaticoside at Low Dose on Wound Burn Repair and Its Mechanism, European Journal of Pharmacology, 584, 415-423.

Mosmann T., 1983, Rapid Colorimetrcic Assays for Celluler Growth and Survival : Application to Proliferation and Citotoxicty Assays, Journal of Immunological Methods, 16 (65) : 55-63.

Pitojo S., 2002, Ceplukan Herba Berkhasiat Obat, Kanisius, Yogyakarta.

Punturee K., Wild C.P., Kasinrerk W., Vinitketkumnuen U. 2005, Immunomodulatory Activities of Centella asiatica and Rhinacanthus nasutus extracts, Asian Pacific Journal of Cancer Prevention, 6, 396-400.

Robinson T., 1995, Kandungan Organik Tumbuhan Tinggi, diterjemahkan oleh Kosasih Padmawinata, Edisis VI, 156-157, Penerbit ITB, Bandung.

Widowati L., Pudjiastuti, Indrari, Sundari D. 1992, Beberapa Informasi Khasiat Keamanan dan Fitokimia Tanaman Pegagan, Warta Tumbuhan Obat Indonesia, Jakarta.



DOI: https://doi.org/10.22146/tradmedj.8861

Article Metrics

Abstract views : 412 | views : 404

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada