The Ameliorative Effects of Phyllanthus gomphocarpus Hook. F. on Sperm Parameters of Rats Induced with Bisphenol A

https://doi.org/10.22146/mot.84823

Evana Kamarudin(1), Razif Dasiman(2*), Ebby Anuar Bahari(3), Kamalru Azman Daud(4), Nur Rohmah Suwandi(5)

(1) Faculty of Health Sciences, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor
(2) *) Faculty of Health Sciences, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor *) Maternofetal and Embryo Research Group (MatE), Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor
(3) Hospital Universiti Teknologi MARA (HUiTM), 42300 Bandar Puncak Alam, Selangor
(4) Department of Pathology, Hospital Tunku Azizah Kuala Lumpur (HKL), Jalan Pahang, 50586 Kuala Lumpur
(5) Faculty of Health Sciences, Universitas Aisyiyah Bandung, Jl. Banteng Dalam No.6, kel Turangga, Kec. Lengkong, Kota Bandung,40264 West Java
(*) Corresponding Author

Abstract


Infertility due to poor sperm quality is a common problem nowadays, particularly in developed countries. Herbs are one of the natural treatment options that have been proposed. Phyllanthus gomphocarpus Hook. F is a tropical plant that has traditionally been used in Malaysia to treat male infertility. Antioxidant characterization of its root aqueous extraction was performed using ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), and total flavonoid content (TFC). Male rats were administered two different concentrations of the extract orally along with BPA for 21 days, and their sperm was evaluated using Computer-Assisted-Sperm-Analysis (CASA). The results showed that this plant has a high antioxidant capacity, with 78.6 ± 0.65 %, 0.14 ± 0.01 mg/ml, 28.52 ± 0.13 mg GAE/g and 164.73 ± 23.13 mg QE/g, for DPPH, FRAP, TPC and TFC respectively. The ability of this plant to enhance sperm concentration, motility, velocity, progression ratio, elongation, and percentages of normal sperm morphology significantly with p<0.05, appears to be due to its high antioxidant capacity. Furthermore, this plant was found to have sperm-protective effects against the endocrine disruptor BPA. A thorough investigation is required to determine the full mechanism of this plant's beneficial effects on male fertility and human health.


Keywords


Infertility; sperm parameters; Phyllanthus gomphocarpus; antioxidant; Computer-Assisted-Sperm-Analysis (CASA)

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DOI: https://doi.org/10.22146/mot.84823

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