SCREENING AND TLC-BIOAUTOGRAPHY ANALYSIS OF ANTIMICROBIAL COMPOUNDS FROM SOME SPONGE EXTRACTS ORIGINATED FROM BARRANG LOMPO SEA ISLAND, SOUTH SULAWESI

https://doi.org/10.22146/tradmedj.8345

Risfah Yulianty(1*), Herlina Rante(2), Gemini Alam(3), Akbar Tahir(4)

(1) Faculty of Pharmacy, Universitas Hasanuddin
(2) Faculty of Pharmacy, Universitas Hasanuddin
(3) Faculty of Pharmacy, Universitas Hasanuddin
(4) Faculty of Marine Science, Universitas Hasanuddin
(*) Corresponding Author

Abstract


Sponges is one of the invertebrate Porifera phyla that produce active compounds with various structures and one of the biological activity as antimicrobial. The purpose of this research was to find out the sponge extracts that can inhibit microbial growth and potential of antimicrobial active compounds from sponge at Barrang Lompo Island. The microbes used were Escherichia coli, Salmonella typhii, Staphylococcus aureus, and Candida albicans. The sponge extracts were obtained by maceration 14 sponge samples with methanol, followed by partition using chloroform and methanol, then TLC-bioautography toward the active extract. The results showed that methanol extracts of sponge with code BRLP-009 and 010 have the most potential effect as antimicrobial agent.

Keywords


TLC-Bioautography, antimicrobial, sponges



References

Alam, G., Adnan, A., Makhmud, A.I., Djide M.N., 2003, Analisis KLT Bioautografi Senyawa Antibakteri Ekstrak Metanol Spons Callyspongia sp. Majalah Obat Tradisional

Ang, K.K.H., Holmes, M.J., Higa. M.T., Kara, U.A.K. 2000. In Vivo Antimalarial Activity of The Beta-Carboline Alkaloid Manzamine A. Antimicrobial Agents and Chemotheraphy. 1645-1649

Astuti, P., Alam, G., Pratiwi,S.U.T., Hertiani, T., Wahyuono S. 2003. Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado. Majalah Obat Tradisional. 8(2): 36 – 38

Endrada, R.U., Proksch, P., Wray, V., Witte, L., Muller, W.E.G. Soest, R., van. R.W.M., 1996. Four New Bioactive Manzamine-Type Alkaloids from The Phillippine Marine Sponge Xestospongia ashmorica. J. of Natural Product. 59. 1056-1060

Hamburger, M.O., dan Cordell, G.A. 1987. Direct Bioautographic TLC Assay for Compounds Possesing Antibacterial Activity. Natural

Product. 50(1):19-22.

Harborne, J.B. 1984. Metode Fitokimia. Terjemahan oleh Kosasih Padmawinata dan Iwang Soediro. 1996. ITB. Bandung. 6-10, 19-20

Jawetz, Melnick, dan Adelbergs’s. 2001. Mikrobiologi Kedokteran. Bagian

Mikrobiologi Fakultas Kedokteran. Universitas Airlangga. Salemba Medika. Jakarta. 224-228

Kelly, M. 2001. A Course Guide to The Sponge Taxonomy Workshop. Departement of Pharmacognosy. Biological Field Station. University of Mississippi and NIWA, New Zealand

Kozloff, E.N. 1990. Invertebrates, 74. Sounders College Publishing. Philadelphia. 86-89

Linington, R.G., Robertson, M., Gauthier, A., Finlay, B.B., Soest, R. van.,Andersen, R.J. 2002. Caminoside A, An Antimicrobial Glycolipid Isolated from The Marine Sponge Caminus sphaeroconia. Org Lett. Nov 14; 4(23). 4089-4092

Nakao, Y., Uehara, T., Matsunaga, S., Fusetani, N., van Soest, R.W., Matunaga, S. 2003. Callysponginic Acid, A Polyacetylenic Acid which Inhibits Alpha Glucosidase, from The Marine Sponge Callyspongia truncata. Journal of Natural Product. 66(1). 156

Rachmaniar, R. 2003. Antikanker Swinholide A dari Spons Theonella swinhoei. Jurnal Bahan Alam Indonesia. 2(4):122



DOI: https://doi.org/10.22146/tradmedj.8345

Article Metrics

Abstract views : 2188 | views : 6132

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada