ANTIOXIDANT AND ANTITUMOR ACTIVITY OF INDONESIAN HERBAL INGREDIENTS

https://doi.org/10.22146/tradmedj.8339

Mahfudz L. D.(1*), R. Murwani(2), Kusumowardani T. H.(3), D. X. Hou(4)

(1) Faculty of Animal Sciences, Diponegoro University
(2) Faculty of Animal Sciences, Diponegoro University
(3) Faculty of Animal Sciences, Diponegoro University
(4) 
(*) Corresponding Author

Abstract


The present study was to aimed to evaluated and compare in vitro antioxidant activities of 2 Indonesian herbal ingredients (A and B), determined total phenol content., cytotoxic and apoptosis induction activities on HL-60 cells. These data were providing some useful information for people healthy dietary and the new potential application of natural antioxidant containing food materials in functional foods and also as new cancer therapeutics promising candidates. The parameters were total antioxidant activity, amount of total cytotoxic effect on the growth of human promyelocytic leukemia cells (HL-60). Statistical comparison was perform with Student’s t-test at p<0.05. The correlation coefficient (r2) between the parameters tested was established by regression analysis. The scavenging effect of extracts herbal on DPPH radicals increased from 0.3-1.5 mg/ml, where is sample A 14.33% to 64.29% and sample B 9.09% to 57.53% was obtained. High content of total phenol compounds were in sample A (21.72 mg GAE/g), lower amounts were in sample B (17.53 mg GAE/g). Apoptosis of HL-60 cells from the morphological changes side (chromatin condensation). Chromatin condensation, a specific and distinct feature of apoptotic cells, was found in the majority of treated cells. The results indicated that the cell death receptor pathway was involved in the apoptosis induced by Indonesian herbal extracts.

Keywords


Indonesian herbal, antioxidant, apoptosis of HL-60 cells

Full Text:

PDF


References

Arul, V., Miyazaki, S. and Dhananjayan, R.. 2005. Studies on the anti inflammatory, antipyretic and analgesic properties of the leaves of Aegle marmelos Corr. J. of Ethmopharmacology 96 : 159-163

Banerji, A., Sarkar, M. and Datta, R. 2002. Amides from Piper brachytachyum and Piper retrofractum. Phytochemistry 59 : 897-901

Claeson, P. and Panthong A. 1993. Three nonphenolic diarylheptanoids with antiinflammatory activity from Curcuma xanthorrhiza. Planta Medica 59(5): 451-454

Claeson, P. and Pongprayoon, U. 1996. Nonphenolic linear diarylhepatonoids from Curcuma xanthorrhiza: A novel type of topical anti-inflammatory agents: Structureactivity relationship. Planta Medica 62(3): 236-240

Elfahmi. 2006. Phytochemical and Biosynthetic Studies of Lignans: with a Focus on Indonesian Medicinal Plants, Facilitair Bedrijf, Univ of Groningen, the Netherlands

Ficker, C.E., Smith, M.L., Susiarti, S., Leaman, D.J., Irawati, C. and Arnason, J.T. 2003. Inhibition of human pathogenic fungi by members of Zingiberaceae used by the Kenyah (Indonesian Borneo). J. of Ethnopharmacol, 85: 289-293

Handayani, V.R.. 1994. Uji aktivitas antiinflamasi ekstrak Zingiber aromaticun rhizoma, Zingiberis zerummbeti rhizoma, dan Zingiberis americanis rhizoma pada tikus putih. Laporan Penelitian Puslitbang

Farmasi, Balitbangkes, Depkes Rl

Hou, D. –X., Ose, T., Lin, S., Harazoro, K., Imamura, I., Kubo, M., Uto, T., Terahara, N., Yoshimoto, M. And Fujii, M. 2003. Anthocyanidins induce apoptosis in human promyelocytic leukemia cells: Structure-activity relationship and mechanism involved. Int. J. Oncol. 23: 705-712

Hwang, J. K. and Shim, J. S. 2000. Xanthorrhizol: A potential antibacterial agent from Curcuma xanthorrhiza against Streptococcus mutans. Planta Medica, 66(2): 196-197

ISM. 2007. Standardization of Indonesian Extracts Herbal. PT. ISM Company Profile, Indonesia. (Unpublished)

Jagetia, G.C., Venkatesh, P. and Baliga, M.S. 2005. Aegle marmelos (L) Correa Inhibits The Proliferation of Transplanted Ehrlich Ascites Carcinoma in Mice. Biol. Pharm. Bull. 28 (1) : 58-64

Kirana, C., McIntosh, G.H., Record, I.R. and Jones, G.P. 2003. Antitumor activity of extract of Zingiber aromaticum and its bioactive sesquiterpenoid zerumbone. Nutr. Cancer 45 (2) : 218-225

Lin, S.C. and Lin, C.C. 1995. Protective and therapeutic effects of Curcuma xanthorrhiza on hepatotoxin-induced liver damage. Am J.of Chinese Med 23(3-4): 243-254

Liu, J., Li, Y., Ren, W. and Hu, W.X. 2006. Apoptosis of HL-60 cells induced by extracts from Narcissus tazetta var. chinensis. Cancer Letter 242: 133-140

Liu, H., Qiu, N., Ding, H and Yao, R. 2008. Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Research International 41 : 363-370

Masuda, T. and Isobe, J. 1992, Antioxidative curcuminoids from rhizomes of Curcuma xanthorrhiza. Phytochemistry 31(10): 3645-3647

Otake, T., Mori, H., Morimoto, M. and Ueba, N. 1995. Screening of Indonesia plant extracts for antihuman immunodeficiency virus-type 1

(HIV-1) activity. Phytother. Res. 9: 6-10

Pan, M.H., Huang, T.M. and Lin, J.K. 1999. Biotransformation of curcumin through reduction and glucuronidation in mice. The American Society for Pharmacology and Experimental Therapeutics 0090-9556/99/2701-0486–494$02.00-/0, Available online at http://www.dmd.org

Rabah, I.O., Hou, D.X., Komine, S., and Fujii, M. 2004. Potential chemopreventive properties of extract from baked sweet potato (Ipomoea Batatas Lam. Cv. Koganesengan). J. Agric. Food Chem. 52: 7152-7157

Rabah, I.O., Hou, D.X., Komine, S.I., Shono, M. and Fujii, M. 2005. Increase in antioxidant and cytotoxicity through apoptosis-induction on HL-60 of sweet potato (Ipomoea Batatas Lam. Cv. Koganesengan) by sub-critical water treatmnent. Food Sci. Technol. Res. 11 (1): 122-126

Rajadurai M, Padmanabhan, M. and Prince, P.S.M. 2005. Effect of Aegle marmelos leaf extract and α-tocopherol on lipid peroxidation and antioxidants in isoproterenol induced myocardial infarction in rats. Cardiol 1:40-5

Riberio, S.M.R., Barbosa, L.C.A., Queroz, J.H., Knodler, M. and Schieber, A. 2008. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chemistry, 110 : 620-626

Silva, B.A., Ferreres, F., Malva, J.O. and Dias, A.C.P. 2005. Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry, 90: 157-167

Singh R.P., Banerjee, S. and Rao, A.R. 2000. Effect of Aegle marmelos on bio-transformation enzyme systems and protection against free-radical-mediated damage in mice. J Pharm Pharmacol. 52: 991-1000

Yasni, S. and Imaizumi, K. 1991. Effects of an Indonesian medicinal plant, Curcuma xanthorrhiza Roxb., on the levels of serum glucose and triglyceride, fatty acid desaturation, and bile acid excretion in streptozotocin-induced diabetic rats. Agr. and Biol Chem 55(12): 3005-3010

Zhang, Q., Wu, J., Z. Hu and D. Li. 2004. Induction of HL-60 Apoptosis by Ethyl Acetate Extract of Cordyceps sinensis Fungal Mycelium. Life

Science 75 : 2911-2919



DOI: https://doi.org/10.22146/tradmedj.8339

Article Metrics

Abstract views : 2154 | views : 1844

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada