ANTIOXIDANT ACTIVITY OF KESEMEK LEAVES (Diospyros kaki L.F) USING DPPH (2,2-DIPHENYL-1 PIKRYLHYDRAZINE) METHOD

https://doi.org/10.22146/tradmedj.8338

Isnindar Isnindar(1*), Erna Prawita Setyowati(2), Subagus Wahyuono(3)

(1) Department of Pharmacy, Faculty of Pharmaceutical and Nursing Science, Universitas Tanjungpura
(2) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
(3) Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


There are two groups of antioxidant resources, these are synthetic and natural antioxidants. The most important natural antioxidant is phenolic compounds that are produced naturally from plants. Phenolic Antioxidants are potent antiradical, biologically active as they are able to donate hydrogens to free radicals and break the chain of lipid oxidation reactions in the early stages. This study is aimed to search natural antioxidant compounds from the leaves persimmon (Diospyros kaki L.F) that have been utilized traditionally to prevent from sickness. In order to detect the presence of antioxidant compounds in the leaves extract, a reagent of DPPH (2,2-diphenyl-1-pykrilhidrazine) in methanol was used. Initially, powder of persimmon leaves (115 g) is macerated 3 times with methanol, 24 hours each time at room temperature. The liquid extract obtained is evaporated by rotavapor till residue (methanol extract) (phase I) is obtained. The residue was then extracted with CHCl3 to give CHCl3 soluble (phase II) and insoluble fractions. The later is then extracted with water to give water soluble (phase III) and insoluble fractions. These fractions (phase I-III) are chromatophed by TLC and sprayed by DPPH. Phase II contains antiradical compounds as shown by yellow color on the violet background. By preparative TLC [SiO2, CHCl3-EtOAc (1-4 v/v)], antiradical substance is isolated and identified tentatively as substituted phenolic compounds. Antiradical potential is
measured spectrophotometrically, the isolat shows IC50 at 107.7 μg/mL that is lower than that of vitamin C (3.04 μg/mL).


Keywords


Persimmon leaves (Diospyros kaki L.F), antioxidant, DPPH (2,2-diphenyl-1-pikrylhydrazine)



References

Ahn HS, Jeon TI, Lee JY, Hwang SG, Lim YH, Park DK (2002). Antioxidative activity of persimmon and grape seed extract: in vitro and in vivo.Nutr. Res. 22:1265-1273

Demirezer, L.O., Kruuzum-Uz, A., Bergere, I., Schiewe, H.J., & Zeeck, A., 2001, The Structures of Antioxidant and Cytotoxic Agents from Natural Source : Antraquinones and Tannin from Roots of Rumex patientia, Phytochemistry, 58: 1213-1217

Fukai S, Tanimoto S, Maeda A, Fukuda H, Okada Y, Nomura M (2009). Pharmacological activity of compounds extracted from persimmon peel (diospyros kaki THUNB.). J. Oleo Sci. 58:213-219

Gali, H. U., Perchellet, E. M., Klish, D. S., Johnson, J. M., and Perchellet, J. P. (1992). Hidrilyzable tannins: potent inhibitors of hydroperoxide production and tumor promotion in mouse skin treated with 12-0 tetradecanoylphobol-13-acetate in vivo. International Journal of Cancer, 51, 425-432

Gorinstein, S., Zemser, M., Weist, M., Halevy, S., Deutsch, J., Tilus, K., Feintuch, D., Guerra, N., Fishman, M., and Bartnikowska, E (1994), Bioscience, Biotechnology and Biochemistry, 58, 1087-1092

Javanmardi, J., Stushnoff, C., Locke, E., Vivanco, JM. Antioxidant Activity and Total Phenolic Content of Iranian Ocimum Accessions. J Food Chem. 2003. (83) : 547-50

Kwon, Y.S., and Kim, C.M., 2003, Antioksidant Constituents from the Steam of Sorghum bicolor, Arch. Pharm. Res., 26 (7) : 535-539

Mowat, A. 1990. Charting the future, proceedings of the first national non-asrtingent persimmon industry workshop. Gatton College: The university of Queensland

Steinmetz KA, and Potter JD (1996). Vegetables, fruit, and cancer prevention: a review. J. Am. Diet. Assoc. 53: 536-543

Supriadi, 2001, Tumbuhan Obat Indonesia ; Penggunaan dan khasiatnya, hal.56-58, Yayasan Obor Indonesia, Jakarta



DOI: https://doi.org/10.22146/tradmedj.8338

Article Metrics

Abstract views : 254 | views : 456

Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

©Majalah Obat Tradisional (Trad.Med.J) 
Faculty of Pharmacy
Universitas Gadjah Mada