The Effect of Surfactant Concentration to Particle Size and Loading Dose of Immunity Jamu’s Ethanolic Extract SNEDDS (Self-Nano Emulsifying Drugs Delivery System)

https://doi.org/10.22146/mot.83321

Dian Eka Ermawati(1*), Samrotul Jannah(2)

(1) Department of Pharmacy, Mathematics and Natural Science Faculty, Universitas Sebelas Maret, Surakarta
(2) Department of Pharmacy, Vocasional School, Universitas Sebelas Maret, Surakarta, Central Java
(*) Corresponding Author

Abstract


Immunity jamu consists of ginger, turmeric, Centella, and cinnamon, that act as immunostimulant agents. However, the infusion is impractical and used a limited dose of the extract, so it is necessary to develop drug delivery to resolve that problem. The SNEDDS technique is expected to increase the solubility, drug release, and absorption of active substances in the body, especially for low solubility of an active substance. SNEDDS consists of oil, surfactant, and co-surfactant. A surfactant is a substance that can reduce surface tension so that emulsion globules form in nanoparticle size. Tween 80 can produce a more transparent solution for oil-in-water emulsions than surfactants with low HLB values. This study aimed to determine the effect of surfactant concentration on the physical properties of SNEDDS to obtain the most loading dose but the smallest particle size. The formula consists of tamanu oil tween 80-propylene glycol of 1:7:1; 1:8:1; and 1:9:1 that incorporated extracts were 75, 150, and 375 mg. The physical tests included transmittance percentage, emulsification time on AGF media, phase separation, and stability test using the cycling test method. SNEDDS, then followed by the Particle Size Analyzer test. The results showed that the greater surfactant concentration produced a better transmittance value, a faster emulsification time, and stability. Formula with oil: surfactant: co-surfactant of 1:8:1 is a system that meets the requirement for immunity jamu with an optimal loading dose and small particle size compared to another formula. The extract’s loading dose of 375 mg has a particle size of 27.17 nm and a polydispersity index of 0.25.


Keywords


Immunity jamu; immunostimulant; surfactant; SNEDDS; loading dose

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References

Akbar, N. D., Nugroho, A. K., & Martono, S, 2022, by simplex lattice design and box behnken design, Jurnal Ilmiah Farmako Bahari. 13(1).

Anggoro, D., Rezki, R. S., & Siswarni, M. Z, 2015, Ekstraksi Multi Tahap Kurkumin dari Temulawak (Curcuma xanthorriza Roxb) Menggunakan Pelarut Etanol, Jurnal Teknik Kimia USU. 4(2), 39-45.

Artanti, A. N., Prihapsara, F., Ermawati, D. E., & Shofa, A. S, 2020, Optimization of Self-Micro Emulsifying Drug Delivery System) for Soursop Leaf (Annona muricata Linn.) Chloroform Extract, Majalah Obat Tradisional. 25(2), 81-89.

Artanti, A. N., Prihapsara, F., Mas' ud, N., & Ermawati, D. E, 2021, Optimization of the Proportion of Surfactant, Co-Surfactant, and Candlenut Oil for Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Secang Heartwood (Caesalpinia sappan L.) Methanolic Extract, ALCHEMY Jurnal Penelitian Kimia. 17(1), 124-131.

Azeem, A., Rizwan, M., Ahmad, F. J., Iqbal, Z., Khar, R. K., Aqil, M., & Talegaonkar, S, 2009, Nanoemulsion components screening and selection: a technical note, Aaps Pharmscitech. 10, 69-76.

Date, A. A., Desai, N., Dixit, R., & Nagarsenker, M, 2010, Self-nanoemulsifying drug delivery systems: formulation insights, applications and advances, Nanomedicine. 5(10), 1595-1616.

Desai, S. S., Shruti, K., Sana, S., Sapna, P., Zainab, M., Swati, H., & Hungund, B. S, 2013, Phytochemical analysis, free radical scavenging and antioxidant profiling using chromatographic techniques for Centella asiatica. International Journal of Biotechnology and Bioengineering Research, 4(7), 687-696.

Di, L., & Kerns, E, 2015, Drug-like properties: concepts, structure design and methods from ADME to toxicity optimization. Academic press.

Ermawati, D. E., Sasmito, E., Mufrod, M., ND, P. E., UA, N. P., Anggi, K. D., ... & Aini, S, 2016, Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine, Journal of Food and Pharmaceutical Sciences. 4(3).

Ermawati, D. E., Pambudi, R. K., Aviwiandari, V., Farida, Y., Rohmani, S., Kundarto, W., & Nugraheni, E. R, 2020, Developing formula of SNEDDS (self-nano emulsifying drug delivery system) antihypertensive herbals “Hortus Medicus”, In AIP Conference Proceedings (Vol. 2237, No. 1, p. 020062). AIP Publishing LLC.

Herman, H., & Murniati, M, 2019, Inventarisasi Tanaman Obat Tradisional Untuk Penderita Diabetes Melitus Dan Hipertensi Di Desa Minanga Kecamatan Bambang Kabupaten Mamasa, Jurnal Farmasi Sandi Karsa. 5(1), 26-32.

Hidayah, I., & Indradadi, R. B, 2020, Review artikel aktivitas imunomodulator beberapa tanaman dari suku Zingiberaceae, Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan. Analis Kesehatan dan Farmasi, 20(2), 181-193.

Jafarzadeh, A., Mohammadi-Kordkhayli, M., Ahangar-Parvin, R., Azizi, V., Khoramdel-Azad, H., Shamsizadeh, A., ... & Khaksari, M, 2014, Ginger extracts influence the expression of IL-27 and IL-33 in the central nervous system in experimental autoimmune encephalomyelitis and ameliorates the clinical symptoms of disease, Journal of neuroimmunology. 276(1-2), 80-88.

Jung, Y. S., Park, S. J., Park, J. H., Jhee, K. H., Lee, I. S., & Yang, S. A, 2012, Effects of ethanol extracts from Zingiber officinale Rosc., Curcuma longa L., and Curcuma aromatica Salisb. on acetylcholinesterase and antioxidant activities as well as GABA contents. Journal of the Korean Society of Food Science and Nutrition, 41(10), 1395-1401

Kautsari, S. N., Purwakusumah, E. D., & Nurcholis, W, 2021, Profil kromatografi lapis tipis ekstrak kunyit (curcuma longa linn) segar dan simplisia dengan variasi metode ekstraksi, Media Farmasi. 16(1), 65-70.

Khairunnisa, S., Hakim, A. R., & Audina, M, 2022, Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol Dari Ekstrak Daun Pegagan (Centella asiatica [L] Urban): Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol Dari Ekstrak Daun Pegagan (Centella asiatica [L] Urban), Journal Pharmaceutical Care and Sciences. 3(1), 121-131.

Lesmana, H., Alfianur, A., Utami, P. A., Retnowati, Y., & Darni, D, 2018, Pengobatan tradisional pada masyarakat tidung kota Tarakan: study kualitatif kearifan lokal bidang Kesehatan, Medisains. 16(1), 31-41.

Li, M., Yue, G. G. L., Tsui, S. K. W., Fung, K. P., & Bik-San Lau, C, 2018, Turmeric extract, with absorbable curcumin, has potent anti-metastatic effect in vitro and in vivo, Phytomedicine. 46, 131-141.

Mohd Zainol, M. K., Abdul-Hamid, A., Abu Bakar, F., & Pak Dek, S, 2009, Effect of different drying methods on the degradation of selected flavonoids in Centella asiatica. International Food Research Journal, 16(4), 531-537.

Patel, M. J., Patel, N. M., Patel, R. B., & Patel, R. P, 2010, Formulation and evaluation of self-microemulsifying drug delivery system of lovastatin, Asian J Pharm Sci. 5(6), 266-275.

Pratiwi, L., Fudholi, A., Martien, R., & Pramono, S, 2017, Self-nanoemulsifying Drug Delivery System (Snedds) for topical delivery of mangosteen peels (Garcinia mangostana L.,): Formulation design and in vitro studies, Journal of Young Pharmacists. 9(3), 341.

Prihapsara, F., Harini, M., Widiyani, T., Artanti, A. N., & Ani, I. L, 2017, Antidiabetic activity of self-nanoemulsifying drug delivery system from bay leaves (Eugenia polyantha Wight) ethyl acetate fraction. In IOP Conference Series: Materials Science and Engineering (Vol. 176, No. 1, p. 012004). IOP Publishing.

Quyen, N. T. C., Quyen, N. T. N., Quy, N. N., & Quan, P. M, 2020, Evaluation of total polyphenol content, total flavonoid content, and antioxidant activity of Centella asiatica. In IOP Conference Series: Materials Science and Engineering (Vol. 991, No. 1, p. 012020). IOP Publishing.

Rahayu, D. U. C., Hakim, R. A., Mawarni, S. A., & Satriani, A. R, 2022, Indonesian Cinnamon (Cinnamomum burmannii): Extraction, Flavonoid Content, Antioxidant Activity, and Stability in the Presence of Ascorbic Acid. Cosmetics, 9(3), 57.

Revathy, S., Elumalai, S., & Antony, M. B, 2011, Isolation, purification and identification of curcuminoids from turmeric (Curcuma longa L.) by column chromatography. Journal of Experimental sciences, 2(7).

Rohmah, S. A. A., Muadifah, A., & Martha, R. D, 2021, Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat pada Sari Kedelai di Beberapa Kecamatan di Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis, Jurnal Sains dan Kesehatan (J. Sains Kes.). 3(2), 120-127.

Rowe, R. C., Sheskey, P., & Quinn, M, 2009, Handbook of pharmaceutical excipients. Libros Digitales-Pharmaceutical Press.

Saidah, S., Kholifah, K., Wahyuni, L., Rahmatika, A. M., & Ulfah, M, 2017, atraksi louis: aktivitas kombinasi ekstrak sambiloto (andrographispaniculata) dan kayu manis (cinnamomumverum, sin. c. zeylanicum) terhadap sistem imun humoral (igg dan igm) pada mencit balb/c yang diinduksi vaksin hepatitis b, Jurnal Inovasi Teknik Kimia.2(2).

Shofi, M., Suwitasari, F., & Istiqomah, N, 2020, Aktivitas antioksidan ekstrak etanol kamboja jepang (Adenium obesum) dan kamboja putih (Plumeria acuminata), Al-Kauniyah J Biol. 13(2), 167-178.

Shoviantari, F., Liziarmezilia, Z., Bahing, A., & Agustina, L, 2019, Uji aktivitas tonik rambut nanoemulsi minyak kemiri (Aleurites moluccana L.), Jurnal Farmasi dan Ilmu Kefarmasian Indonesia. 6(2), 69-73.

Sogi, D. S., Sharma, S., Oberoi, D. P. S., & Wani, I. A, 2010, Effect of extraction parameters on curcumin yield from turmeric. Journal of food science and technology, 47, 300-304.

Sonam, M., Singh, R. P., & Pooja, S, 2017, Phytochemical screening and TLC profiling of various extracts of Reinwardtia indica. International Journal of Pharmacognosy and Phytochemical Research, 9(4), 523-527.

Syafitri, N. E., Bintang, M., & Falah, S, 2014, Kandungan fitokimia, total fenol, dan total flavonoid ekstrak buah harendong (Melastoma affine D. Don), Current Biochemistry. 1(3).

Trinidad, T. P., Sagum, R. S., de Leon, M. P., Mallillin, A. C., & Borlagdan, M. P, 2012, Zingiber officinale and Curcuma longa as potential functional foods/ingredients. Food and Public Health, 2(2), 1-4.

Widodo, F., Lyrawati, D., & Suryana, B. P. P, 2016, Potential of Topical Curcumin in Reduction of TNF-α expression and Synovium Hyperplasia on Wistar Rats of Rheumatoid Arthritis Model, Research Journal of Life Science. 3(1), 40-48.

Yupitasari, Y., Suryani, D., Wahyudi, A., Krisnasary, A., & Yunita, Y, 2021, Gambaran Konsumsi Karbohidrat, Lemak, Natrium dan Kejadian Hipertensi pada Lansia di Panti Sosial Tresna Werdha Pagar Dewa Kota Bengkulu Tahun 2021 (Doctoral dissertation, Poltekkes Kemenkes Bengkulu).

Zulfa, E., Novianto, D., & Setiawan, D, 2019, Formulasi Nanoemulsi Natrium Diklofenak Dengan Variasi Kombinasi Tween 80 Dan Span 80: Kajian Karakteristik Fisik Sediaan, Media Farmasi Indonesia. 14(1), 1471-1477.



DOI: https://doi.org/10.22146/mot.83321

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