ANTIANGIOGENIC EFFECT OF ETHANOLIC EXTRACT OF GREEN ALGAE (Spirogyra sp.) AGAINST EXPRESSION COX-2 IN T47D CELLS

https://doi.org/10.22146/tradmedj.8229

Wahyu Widyaningsih(1*), Nina Salamah(2), Hari Susanti(3), Dwi Fitriani(4)

(1) Faculty of Pharmacy, Universitas Ahmad Dahlan
(2) Faculty of Pharmacy, Universitas Ahmad Dahlan
(3) Faculty of Pharmacy, Universitas Ahmad Dahlan
(4) Faculty of Pharmacy, Universitas Ahmad Dahlan
(*) Corresponding Author

Abstract


Cancer is a group of diseases that arise when a cell or group of cells that regulate out of control growth. Green algae (Spirogyra sp.) is one of the medicinal plants used in traditional medicine for the treatment of cancer. Green algae (Spirogyra sp.) has active substances such as melatonin. Melatonin which is a compound that has been examined by researchers world as anticancer drugs and antioxidants. This study aims to determine the effect of ethanol extract of green algae (Spirogyra sp.) on the expression of COX - 2 in T47D cells. Green algae was extracted using a Soxhlet apparatus with 96 % ethanol. This study used three groups: control cells , ethanol extract of green algae concentrations 247.668 ug / mL and 123.834 mg / mL. To ensure the expression of caspase-3 and Bcl-2, test was conducted using indirect immunocytochemistry. Observations were carried out using a light microscope to see and count the percent expression of COX - 2. The results obtained showed significant difference, decreased expression of COX - 2 in each group. It can be concluded that the ethanol extract of green algae (Spirogyra sp.) decreased COX - 2 expression in T47D cells.


Keywords


Green algae, Spyrogyra sp., COX-2, T47D cells, immunocytochemistry

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References

King, R. J. B., 2000, Cancer Biology 2nd ed, Pearson Education Limited, London

Sujudi,Achmad. 2003. Investasi kesehatan untuk pembangunan ekonomi. departeman kesehatan RI.Jakarta

Ganiswara, S., Setiabudy, R., Sjamsudin, U., Bustami, Z., 1995, Farmakologi dan Terapi, Ed Ke4, Bagian Farmakologi Universitas Indonesia, Jakarta

Taskin, E., dan Kurt, O., 2007.Antibacterial activities of some marine algae from the Aegean Sea (Turkey).African Journal of Biotechnology 6(24) 274-275

Kolar J, Machanckova I. 2001.Occurrence and possible function of melantonin in plants. Endocytobiosis and Cell Res 14 (1) : 75-84

Folkman , J., 1996, Tumor angiogenesis therapeutic implications, N. Engl. J. Med., 285: 1182-1186

Prabowo A, 2009, Pemanfaatan phytomelatonin ganggang hijau (Spirogyra Sp.) sebagai cancer activity inhibitor dari induksi logam berat, PKM , Universitas Ahmad Dahlan, Yogyakarta

Breyer B., Jiang, W., Cheng, H., Zhou, L., Paul, R., Feng, T., He, T-C., 2001, Adenoviral vector-mediated gene transfer for human gene therapy, Curr Gene Ther, 1:149-162

Lema, M.J., 2002, Coxib Therapy and Colorectal Cancer: Emerging options with coxib therapy, Cleve Clin J Med, 69: 176-184

Toi, M., Kondo, S., Suzuki, H., Yamamoto, Y., Inada, K., Imazawa, T., Taniguchi, T. and Tominaga, T., 1996, Quantitative analysis of vascular endothelial Growth factor in primary breast cancer. Cancer, 77: 1101-1106

Sobolewski, C., Claudia, C., Mario, D., Lina, G., Marc, D., 2010, The role of cyclooxygenase-2 in cell proliferation and cell death in human malignancies, International journal of cell biology

Kerbel, R., and Folkman J., 2001, Clinical Translation of Angiogenesis inhibitor, Natrure Rev., 2: 727-739



DOI: https://doi.org/10.22146/tradmedj.8229

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