.png)
.png)
* Corresponding Author
Analysis of Curcumin Contents in Curcuma xanthorrhiza using FTIR Spectroscopy and HPLC-UV in Combination with Multivariate Calibration
https://doi.org/10.22146/mot.82220 Awal P. Kusumadewi(1), Rohmat Mujahid(2), Ronny Martien(3), Purwanto Purwanto(4), Anita A. Styawan(5), Irnawati Irnawati(6*), Abdul Rohman(7)
(1) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Medicinal Plant and Traditional Medicine Research and Development Center, Tawangmangu, Central Java
(2) Research Center for Pharmaceutical Ingredients and Traditional Medicine of National Research and Innovation Agency (BRIN), Indonesia
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(5) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Faculty of Health and Technology, Universitas Muhammadiyah Klaten, Central Java
(6) Study Program of Pharmacy, Faculty of Pharmacy, Halu Oleo University, Kendari
(7) *) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta *) Centre of Excellence Institute for Halal Industry and Systems (PUI-PT IHIS), Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
(*) Corresponding Author
Abstract
Curcuma xanthorrhiza Roxb. or Java Turmeric with the local name of Temulawak is one of herbal medicines used in Indonesia. This plant is believed to prevent some degenerative diseases due to its active compounds, especially curcumin (CUR) contained in Java Turmeric. Therefore, analysis of active components including CUR is very urgent. This study highlighted the development of FTIR spectroscopy coupled with PLSR for the determination of CUR in Java Turmeric powders. The levels of CUR in Java Turmeric powders were determined using HPLC with UV detectors, and the obtained results were used as actual values to be predicted using FTIR spectroscopy-multivariate calibrations. The results revealed that the levels of CUR ranged from 0.6741 ± 0.0705% (g/100 samples) to 2.1062 ± 0.0095%. PLSR modeling for the relationship between the actual value of CUR as determined using HPLC and calculated values as predicted using FTIR spectroscopy provide the value of R2 of 0.9990 with RMSEC of 0.0028. The developed method offers reliable results providing a green analytical method due to the use of minimum solvent and reagent and does not involve extensive sample preparation.
Keywords
Full Text:
PDFReferences
Akarchariya, N., Sirilun, S., Julsrigival, J., & Chansakaowa, S., 2017, ‘Chemical profiling and antimicrobial activity of essential oil from Curcuma aeruginosa Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xanthorrhiza Roxb. collected in Thailand’. Asian Pac. J. Trop. Biomed. 7(10), 881–885.
Batista de Andrade Neto, J., Pessoa de Farias Cabral, V., Brito Nogueira, L. F., Rocha da Silva, C., Gurgel do Amaral Valente Sá, L., Ramos da Silva, A., Barbosa da Silva, W. M., Silva, J., Marinho, E. S., Cavalcanti, B. C., Odorico de Moraes, M., & Nobre Júnior, H. V., 2021, ‘Anti-MRSA activity of curcumin in planktonic cells and biofilms and determination of possible action mechanisms’, Microb. Pathog. 155, 104892.
Cramer, R. D., 1993, Partial Least Squares (PLS): Its strengths and limitations. Perspectives Drug Discover. Design, 1(2), 269–278.
Gören, A. C., Çikrikçi, S., Çergel, M., & Bilsel, G., 2009, ‘Rapid quantitation of curcumin in turmeric via NMR and LC-tandem mass spectrometry’, Food Chem. 113(4), 1239–1242.
Khajehsharifi, H., Pourbasheer, E., Tavallali, H., Sarvi, S., & Sadeghi, M., 2017. The comparison of partial least squares and principal component regression in simultaneous spectrophotometric determination of ascorbic acid, dopamine and uric acid in real samples. Arabian J. Chem., 10, S3451–S3458.
Kusumadewi, A. P., Martien, R., Pramono, S., Setyawan, A. A., & Rohman, A., 2022, ‘Review on the application of chemometrics for the standardization and authentication of Curcuma xanthorrhiza’, Food Res. 6(2), 1–8.
Li, Y., Shen, Y., Yao, C., & Guo, D., 2020, ‘Quality assessment of herbal medicines based on chemical fingerprints combined with chemometrics approach: A review’, J.Pharm. Biomed. Anal. 185, 113215.
Long, Y., Zhang, W., Wang, F., & Chen, Z., 2014, ‘Simultaneous determination of three curcuminoids in Curcuma longa L. by high performance liquid chromatography coupled with electrochemical detection’, J. Pharm Anal. 4(5), 325–330.
Noori, S., Kiasat, A. R., Kolahi, M., Mirzajani, R., & Seyyed Nejad, S. M., 2022, ‘Determination of secondary metabolites including curcumin in Rheum ribes L. and surveying of its antioxidant and anticancer activity’, J. Saudi Chem. Soc. 26(3), 101479.
Prabaningdyah, N. K., Riyanto, S., & Rohman, A., 2018, ‘Application of FTIR spectroscopy and multivariate calibration for analysis of curcuminoid in syrup formulation’, J. Appl. Pharm. Sci. 8(3), 172-179.
Rafi, M., Wulansari, L., Heryanto, R., Darusman, L. K., Lim, L. W., & Takeuchi, T., 2015, ‘Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector’, Food Anal. Methods, 8(9), 2185–2193.
Rohaeti, E., Rafi, M., Syafitri, U. D., & Heryanto, R., 2015, ‘Fourier transform infrared spectroscopy combined with chemometrics for discrimination of Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar’, Spectrochim Acta A Mol. Biomol Spectrosc. 137, 1244–1249.
Rohman, A., Wijayanti, T., Windarsih, A., & Riyanto, S., 2020a. The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis. Molecules, 25(17), 1-13.
Rohman, A., Widodo, H., Lukitaningsih, E., Windarsih, A., Rafi, M., & Nurrulhidayah, A. F., 2020b, ‘Review on in vitro antioxidant activities of curcuma species commonly used as herbal components in Indonesia’, Food Res. 4(2), 286–293.
Septama, A. W., Tasfiyati, A. N., Kristiana, R., & Jaisi, A. (2022). Chemical profiles of essential oil from Javanese turmeric (Curcuma xanthorrhiza Roxb.), evaluation of its antibacterial and antibiofilm activities against selected clinical isolates. South African J. Botany, 146, 728–734.
Sharma, K., Agrawal, S. S., & Gupta, M., 2012, ‘Development and validation of UV spectrophotometric method for the estimation of curcumin in bulk drug and pharmaceutical dosage forms’, Int. J. Drug Dev. Res. 4(2), 375–380.
Singh, S. K., Jha, S. K., Chaudhary, A., Yadava, R. D. S., & Rai, S. B., 2010, ‘Quality control of herbal medicines by using spectroscopic techniques and multivariate statistical analysis’, Pharm Biol, 48(2), 134–141.
Siregar, C., Martono, S., & Rohman, A., 2018, Application of Fourier transform infrared (FTIR) spectroscopy coupled with multivariate calibration for quantitative analysis of curcuminoid in tablet dosage form. J. Appl. Pharm. Sci, 8(8), 151-156.
Sorng, S., Balayssac, S., Danoun, S., Assemat, G., Mirre, A., Cristofoli, V., Le Lamer, A. C., Jullian, V., Gilard, V., Fabre, N., Martino, R., & Malet-Martino, M., 2022, ‘Quality assessment of Curcuma dietary supplements: Complementary data from LC-MS and 1H NMR’, J. Pharm. Biomed. Anal. 212, 114631.
Syed, H. K., Liew, K. Bin, Loh, G. O. K., & Peh, K. K., 2015, ‘Stability indicating HPLC-UV method for detection of curcumin in Curcuma longa extract and emulsion formulation’, Food Chem. 170, 321–326.
an Wyngaard, E., Blancquaert, E., Nieuwoudt, H., & Aleixandre-Tudo, J. L., 2021. Infrared Spectroscopy and Chemometric Applications for the Qualitative and Quantitative Investigation of Grapevine Organs. Front. Plant Sci., 12, 1-16
Wulandari, R., Sudjadi, Martono, S., & Rohman, A., 2018, ‘Liquid chromatography and fourier transform infrared spectroscopy for quantitative analysis of individual and total curcuminoid in Curcuma longa extract’, J. Appl. Pharm. Sci. 8(9), 107–113.
Xiang, H., Zhang, L., Xi, L., Yang, Y., Wang, X., Lei, D., Zheng, X., & Liu, X. (2018). Phytochemical profiles and bioactivities of essential oils extracted from seven Curcuma herbs. Industrial Crops Products, 111, 298–305. DOI: https://doi.org/10.22146/mot.82220
Article Metrics
Abstract views : 7927
| 
views : 7092
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Majalah Obat Tradisional
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Faculty of Pharmacy
Universitas Gadjah Mada