RADICALS ARREST OF 2,2-diphenyl-1-pycryl hydrazyl (DPPH) IN RIPE AND RAW PAPAYA FRUIT (Carica papaya L. (orentang)
Emir Ramadhan(1*), Sudarsono Sudarsono(2)
(1) Faculty of Pharmacy, Universitas Gadjah Mada
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Some compounds were potentially as a radical catcher, it was also found in the papaya fruit (Carica papaya L. (orentang)). This study aimed to determine 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical capture of ripe and raw papaya fruits with vitamin C synthetic as a benchmark. Mashed papaya pulp was dried by the freeze-drying method. The dryed mass was dissolved in ethanol and reacted with DPPH. Vitamin C was also treated by the same method, then made a standard and curve of percent DPPH radical arrest by vitamin C. Percent DPPH radical capture by the ripe and raw papaya fruit powder extracts was plotted on a standard curve. Ripe papaya fruit had the ability to capture the highest DPPH radical (1.340,931 mg of fresh ripe papaya proportional to 1 mg of vitamin C). The raw papaya had the capability of 3.557,814 mg of fresh raw papaya proportional to 1 mg of vitamin C.
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Anonim, 2010, Pepaya, http:// www.arsgrin.gov: 8080/ npgspub/ xsql/ duke/ plantdisp. xsql/ taxon=209, September 2010
Atun, S., Arianingrum, R., & Aznam, N., 2008, Uji Aktivitas Beberapa Senyawa Oligoresveratrol Hasil Isolasi Dari Kulit Batang Tumbuhan Hopea Odorata Sebagai Pencegah Degradasi 2-Deoksiribosa, Laporan Penelitian, FMIPA UNY, Yogyakarta
Cholisoh, Z., & Utami, W., 2008, Aktivitas Penangkap Radikal Ekstrak Etanol 70% Biji Jengkol, Laporan Penelitian ,Fakultas Farmasi Universitas Muhammadiyah, Surakarta
Hertiani, 2000, Isolasi dan Identifikasi Senyawa Flavonoid Antioksidan dari Daun Plantago major L., Tesis, Program pasca sarjana UGM
Langseth, L., 1995, Oxydant, Antioxydant and disease Prevention, International Life Science , Institute Press Belgium
Molyneux, P., 2004, The Use of The Stable Free Radikal Diphenyl picrilhidrazyl (DPPH) for Estimating Antioksidant Acti vity, Laporan Penelitian, Songklanakarin J. Sci. Technol
Pokorni, J., Yanishlieva, N., and Gor don, M., 2001, Antioxidant in Food; Practical Application, CRC Press, New York
Winarsi, Heri, 2007, Antioksidan Alami dan Radikal Bebas Potensi dan Aplikasinya dalam Kesehatan, Cetakan I, Kanisius, Yogyakarta
DOI: https://doi.org/10.22146/tradmedj.8221
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Faculty of Pharmacy
Universitas Gadjah Mada