DETERMINATION OF ANDROGRAPHOLIDE ISOLATE ACTIVITY TO α-AMYLASE AND α-GLUCOSIDASE USING APOSTOLIDIS AND MAYUR METHOD

https://doi.org/10.22146/tradmedj.8219

Ichwan Ridwan Rais(1*), Agung Giri Samudra(2), Sitarina Widyarini(3), Agung Endro Nugroho(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada Faculty of Pharmacy, Universitas Ahmad Dahlan
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Faculty of Veterinary Medicine, Universitas Gadjah Mada
(4) Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Disorders of carbohydrate metabolism can lead to diabetes mellitus. Carbohydrates  are metabolized in the gastrointestinal tract  into simple glucose and absorbed into the bloodstream and affected blood glucose levels. The absorption process is catalyzed by α- 1 ,4 - glycoside breaking bond  enzyme , namely α - amylase and α -1 ,6 - glycoside breaking bond  enzyme, namely α – glucosidase. They are found in the intestinal cells. Research had been conducted in an effort to develop an alternative treatment of diabetes mellitus by testing the ability of isolates of andrographolide in inhibiting α-amylase activity and α-glucosidase in vitro. Andrographolide isolates showed fairly good activity in inhibiting α-amylase ( IC50 = 1,.49 mg/mL) and weak in inhibiting α-glucosidase (IC50 = 38,86 mg/mL). Inhibition of α-amylase activity is evidence of one mechanism of andrographolide in reducing carbohydrate metabolism that can affect blood glucose levels and indicates that andrographolide is  a potential alternative medicine in addressing diabetes mellitus .


Keywords


andrographolide , α-amylase , α-glucosidase



References

Ali, H., Houghton, P.J., and Soumyanath, A. 2006, α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 107: 449–455 cit. Subramanian, R., Asmawi, M.Z., and Sadikun, A. 2008, In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide, Act. J. Biochim. Pol., 55(2): 391-398

Apostolidis, E., Kwon, Y.I.I., and Shetty, K.. 2007, Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension, Inn. Food Sci. Emer. Technol., 8: 46-54

Aulia, N., 2008, Penetapan Kadar Andrografolid dan Kurkumin dalam Ekstrak Campuran Herba Sambiloto dan Rimpang Kunyit dengan Metode KLT-densitometri, Universitas Airlangga, Skripsi, Surabaya

Chao, W.W. and Lin, B.F., 2010, Isolation and Identification of Bioactive Compounds in Andrographis paniculata (Chuanxinlian), Chinese Medicine Journal, 5: 1-15

Dep Kes RI, 2005, Pharmaceutical Care Untuk Penyakit Diabetes Mellitus, Dep Kes RI, Jakarta. pp:16, 24, 36-46

Depkes RI, 2009, Farmakope Herbal Indonesia, Departemen Kesehatan RI, Jakarta

Katzung, B.G., 2002, Farmakologi Dasar dan Klinik, diterjemahan dari Bahasa Inggris oleh Bagian Farmakologi Fakultas Kedokteran Universitas Airlangga, Salemba Medika, pp: 349, 694-709

Mayur, B., Sandesh, S., Shruti, S., and Sung-Yum, S., 2010, Antioxidant and α-glucosidase inhibitory properties of Carpesium abrotanoides L., Journal of Medicinal Plants Research, 4 (15): 1547-1553

Mycek, M.J., Harvey, R.A., Champe, P.C., and Fisher, B.D., 2001, Farmakologi Ulasan Bergambar Edisi 2, 1995, Cetakan I. Alih bahasa : Prof.dr.H. Azwar Agoes, Widya Medika, 259-263

Niranjan, A., Tewari, S.K., and Lehry, A., 2010, Biological Activities of Kalmegh (A. Paniculata Ness) and Its Active Principles, Indian J. of Nat. Prod. and Res., 1(2): 125-135

Nugroho, A.E., Syamsul, E.S., Andrie, M., Warditiani, K., Lukitaningsih, E., and Pramono, S., 2011, The Antidiabetics of Purified Extract of Andrographis paniculata (Burn.f.) Ness and its Active Compound Andrographolide in High Fructose-Fat Fed Rats. Laporan penelitian hibah IMHERE, Fakultas Farmasi UGM, Yogyakarta

Soediro, S., 1973, Pemeriksaan zat pahit dalam daun sambiloto (Andrographis paniculata Nees dari Bandung, Jawa). Acta Pharmaceutica

Sudarsono, Puidjoarinto, A., Gunawan, D., Wahyuono, S., Donatus, I.A., Drajad, M., Wibowo, S., and Ngatidjan, 2006, Tumbuhan Obat 1. Pusat Penelitian Obat Tradisional, Universitas Gadjah Mada, Yogyakarta. pp: 25-28

Tadera, K., Minami, Y., Takamatsu, K., and Matsuoka, T., 2006, Inhibition of α-Glukosidase and α-Amylase of Flavonoids, J Nutr. Sci. Vitaminol., 52: 149-153

Warditiani, N.K., 2012, ‘Uji Aktivitas Antihiperlipidemia dan Antiaterosklerosis Isolat Andrografolid dan Ekstrak Terpurifikasi Herba Sambiloto (Andrographis paniculata (Burm.f.) Ness) Pada Tikus Diabetes Mellitus Tipe 2 Resistensi Insulin, Tesis, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta

Wibudi, A., Kiranadi, B., Manalu, W., Winarto, A. and Suyono, S. 2008, The Traditional Plant, Andrographis paniculata (Sambiloto) Exhibits Insulin-Releasing Actions In vitro, Acta Med. Indonesian, 40(2)

Zang, X.F. and Tan, B.K., 2000, Antihyperglycemic and antioxidant Properties of Andrographis paniculata in Normal and Diabetic Rats. J. Clin. Exp. Pharmacol. Physiol., 27 (3): 58-63



DOI: https://doi.org/10.22146/tradmedj.8219

Article Metrics

Abstract views : 2848 | views : 5912

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2015 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada